Utilization of peanut shells as substrate for cellulase production in submerged fermentation through Box-Behnken Design

Irfan, Muhammad; Sadia, Safoora; Bakhtawar, Javeria; Shakir, Hafiz Abdullah; Khan, Muhammad Imran; Ali, Shaukat

doi:10.26577/ijbch-2019-i2-4

Cited by 5 publications

(1 citation statement)

References 10 publications

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“…In a recent investigation conducted by Srivastava et al [26], high cellulase production has been noticed in the presence of pomegranate waste in the growth medium for the coculture of two bacterial strains, Cladosporium cladosporioides NS2 and Rhizopus oryzae NS5 under the solid‐state fermentation. Irfan et al [54], in their study, achieved high cellulase production while using peanut shell as a substrate in the growth medium of Bacillus paralicheniformis during the submerged fermentation. Arshad et al [55] have also reported an optimized condition for enhanced cellulase production using dilute sulfuric acid pretreatments of peanut shells by BBD.…”

Section: Discussionmentioning

confidence: 99%

Purified cellulase‐mediated simultaneous sugar utilization by Bacillus albus isolated from Similipal, Odisha, India

2023

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Among 24 isolated cellulolytic bacteria from Similipal Biosphere Reserve, the most efficient isolate was recognized as a strain of Bacillus albus. This strain of B. albus was evaluated for cellulase production and the cellulase activity was measured in submerged fermentation using substrate carboxymethyl cellulose (CMC). Different nutritional (carbon, nitrogen, and metal‐ion sources) and physical variables (pH, temperature, substrate concentration, and incubation time) during the growth of B. albus were optimized to obtain maximum cellulase activity. The highest cellulase activity of 5.79 U/mL for B. albus was observed at pH 6.75, temperature 37.5°C, CMC concentration 8.5 g/L, and 42 h incubation time. Further, supplementation of glucose as a subsidiary carbon source, yeast extract, peptone as nitrogen sources, and MgSO4 and MnSO4 as metal‐ion sources enhance the cellulase activity of B. albus. The purified enzyme was reported to have a molecular weight of ∼54 kDa as determined by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. A zymogram analysis evidenced the cellulase activity of the purified enzyme fractions obtained from diethylaminoethyl cellulose chromatography. The purified cellulase was reported to have an optimum pH and temperature of 7.0°C and 50°C, respectively with a capacity of retaining its 60% residual activity within pH 6.0–8.0 and temperature 30–40°C, respectively. The metal ions, K+ and Na+ were the activators, while Pb2+ and Hg2+ were the inhibitors for the purified cellulase. The purified cellulase showed Km and Vmax values of 0.38 M and 8.19 U/mL, respectively, in presence of the substrate CMC and also simultaneous consumption of both hexose and pentose sugars.

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Section: Discussionmentioning

confidence: 99%

Purified cellulase‐mediated simultaneous sugar utilization by Bacillus albus isolated from Similipal, Odisha, India

2023

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Recent trends on the food wastes valorization to value-added commodities

Mehmood

Nadeem

Bilal

et al. 2021

Advanced Technology for the Conversion of Waste Into Fuels and Chemicals

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Production Optimization and Biochemical Characterization of Cellulase from Geobacillus sp. KP43 Isolated from Hot Spring Water of Nepal

Khadka

Poudel

et al. 2022

BioMed Research International

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This study is aimed at isolating and identifying a thermophilic cellulolytic bacterium from hot spring water and characterizing thermostable cellulase produced by the isolate. Enrichment and culture of water sample was used for isolation of bacterial strains and an isolate with highest cellulase activity was chosen for the production, partial purification, and biochemical characterization of the enzyme. Different staining techniques, enzymatic tests, and 16s ribosomal DNA (16s rDNA) gene sequencing were used for the identification of the isolate. The cellulase producing isolate was Gram positive, motile, and sporulated rod-shaped bacterium growing optimally between 55°C and 65°C. Based on partial 16s rDNA sequence analysis, the isolate was identified as Geobacillus sp. and was designated as Geobacillus sp. KP43. The cellulase enzyme production condition was optimized, and the product was partially purified and biochemically characterized. Optimum cellulase production was observed in 1% carboxymethyl cellulose (CMC) at 55°C. The molecular weight of the enzyme was found to be approximately 66 kDa on 12% SDS-PAGE analysis. Biochemical characterization of partially purified enzyme revealed the temperature optimum of 70°C and activity over a wide pH range. Further, cellulase activity was markedly stimulated by metal ion Fe2+. Apart from cellulases, the isolate also depicted good xylanase, cellobiase, and amylase activities. Thermophilic growth with a variety of extracellular enzyme activities at elevated temperature as well as in a wide pH range showed that the isolated bacteria, Geobacillus sp. KP43, can withstand the harsh environmental condition, which makes this organism suitable for enzyme production for various biotechnological and industrial applications.

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Utilization of peanut shells as substrate for cellulase production in submerged fermentation through Box-Behnken Design

Cited by 5 publications

References 10 publications

Purified cellulase‐mediated simultaneous sugar utilization by Bacillus albus isolated from Similipal, Odisha, India

Purified cellulase‐mediated simultaneous sugar utilization by Bacillus albus isolated from Similipal, Odisha, India

Recent trends on the food wastes valorization to value-added commodities

Production Optimization and Biochemical Characterization of Cellulase from Geobacillus sp. KP43 Isolated from Hot Spring Water of Nepal

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