Utilization of retrieved oocytes as an index of the efficiency of superovulation strategies for in-vitro fertilization treatment

Meniru, G I; Craft, Ian

doi:10.1093/humrep/12.10.2129

Cited by 49 publications

(32 citation statements)

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“…The total number of high-quality embryos in the present study was much greater following superovulation of cattle with relatively high follicle numbers during waves, but the proportion of high-quality embryos relative to the total number of fertilized oocytes and embryos recovered was greater in animals with relatively low follicle numbers. This observation supports previous reports that the proportion of high-quality embryos is inversely associated with responsiveness of cattle to superovulation (Shaw et al, 1995), and the percentage of embryos transferred declines as number of oocytes harvested increases following IVF and embryo transfer in women (Meniru and Craft, 1997). Since the recovery of embryos and oocytes after artificial insemination was similar for animals with low or high follicle numbers during waves in the present study, the inverse association between response to superovulation and proportion of high-quality embryos could imply that the proportion of follicles with high-quality oocytes during waves may be greater for cattle with low follicle numbers and relatively high circulating FSH concentrations during waves.…”

Section: Discussionsupporting

confidence: 91%

Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle

et al. 2007

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BACKGROUND: The significance of the high variation in numbers of follicles produced during reproductive cycles in humans and cattle is unknown. METHODS: We selected beef heifers with high (25) or low (15) numbers of ovarian follicles and determined the association with alterations in FSH and estradiol concentrations, as well as responsiveness to superstimulation and embryo quality. The variation in follicle numbers was also compared with oocyte quality in natural cycles using IVF and abattoir sourced bovine ovaries. RESULTS: Results show that: (i) FSH was lower (P < 0.03) in animals with high compared with low follicle numbers per follicle wave; (ii) after superovulation, in the high versus low follicle number group, the number of oocytes/embryos recovered after insemination (10.6 + + + + + 2.7 versus 4.7 + + + + + 0.7) and the number of transferable embryos (5.4 + + + + + 1.3 versus 3.8 + + + + + 0.8) per animal were greater (P < 0.05), whereas the proportion of transferable embryos (50.7% versus 79.8%) was lower (P < 0.05); (iii) in unstimulated animals, the numbers of high-quality oocytes harvested and in-vitro fertilized oocytes developing into blastocysts were up to 4-fold greater (P < 0.05) for ovaries with high versus low numbers of follicles, but the proportions of oocytes developing into blastocysts were similar in the two groups. CONCLUSION: Phenotypic classification based on numbers of follicles may be useful to improve superovulation procedures. The lower proportion of transferable embryos following superovulation of ovaries with high numbers of follicles is probably not the result of differences in the quality of oocytes before superovulation.

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Section: Discussionsupporting

confidence: 91%

Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle

et al. 2007

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“…The association between the number of oocytes retrieved and pregnancy rate is controversial. Some researchers have shown a correlation between the number of oocytes retrieved and higher pregnancy rates [50][51][52] whereas others argue that excessive response to ovarian stimulation might result in detrimental effects on oocyte and embryo quality and/or that supra-physiological estradiol levels may alter implantation [53,54]. Of note, we observed a trend towards a lower number of embryos frozen among cohorts with non-cleaved embryos, although the numbers were not statistically significant.…”

Section: Discussioncontrasting

confidence: 67%

Is the presence of a non-cleaved embryo on day 3 associated with poorer quality of the remaining embryos in the cohort?

Machtinger

Bormann

Ginsburg

et al. 2015

J Assist Reprod Genet

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Purpose Morphological evaluation is currently considered the single most important predictive measure for assessing embryo quality. The aim of this study was to investigate whether cycles with at least one non-cleaved embryo (i.e., a 1-cell embryo on day 3) have different outcomes compared with cycles in which all embryos had cleaved by day 3. Methods All autologous IVF/ICSI cycles with a fresh day 3 transfer and without using a gestational carrier performed at our center between 1/1/2010 and 12/31/2011 were analyzed retrospectively. Those cycles with at least one non-cleaved embryo on day 3 were compared with all other autologous cycles that had l00 % cleaved embryos performed during the study period. Results Eight hundred and forty two cycles were included. Of them, 144 cycles comprised the non-cleaved group, and 698 cycles comprised the cleaved group. Cycles in the non-cleaved group had more oocytes retrieved (15.4±7.1 vs. 12.5±7.1, p<0.001), more zygotes obtained (10.0±5.3 vs. 7.9±5.2, p= <0.001), but the embryos exhibited lower cleavage rates and higher rates of fragmentation and asymmetry compared with controls (p<0.001). However, spontaneous abortion rates, ectopic pregnancies rates as well as delivery rates were similar between the two groups.Conclusions Our results show that the presence of a noncleaved embryo on day 3 is associated with a more exuberant response to controlled ovarian stimulation as reflected by more oocytes retrieved. Despite the significant decrease in quality of the whole cohort in the non-cleaved group, implantation, delivery rates and number of embryos frozen were not adversely affected by the presence of a non-cleaved embryo.

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“…In the present study, we report a high total utilization rate of 44.4 % compared to previously reported utilization rates (31.2 % [2]; 38.4 % [25]). Utilization rates are, however, heavily dependent on the criteria for cryopreservation in individual clinics, with liberal criteria resulting in higher utilization rates than with more restrictive criteria.…”

Section: Discussioncontrasting

confidence: 65%

The total pregnancy potential per oocyte aspiration after assisted reproduction—in how many cycles are biologically competent oocytes available?

Lemmen

Rodríguez

Andreasen

et al. 2016

J Assist Reprod Genet

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Purpose While stimulation of women prior to assisted reproduction is associated with increased success rates, the total biological pregnancy potential per stimulation cycle is rarely assessed. Methods Retrospective sequential cohort study of the cumulative live birth rate in 1148 first IVF/ICSI-cycles and 5-year follow up of frozen embryo replacement (FER) cycles were used. Oocyte number, number of embryos transferred, and cryopreserved/ thawed and transferred embryos in a FER cycle were registered for all patients. Children per oocyte and per transferred embryo and percentage of cycles with births were calculated. Results We obtained 9529 oocytes. Embryos (2507) were transferred in either fresh or FER cycles, resulting in 422 births and 474 live born children. Median age of the women was 32.5 years (range 20-41.5 years). In total, 34.3 % of all cycles ended with a live birth while in 65.7 % of the cycles, no oocytes were capable of developing into a child. The average number of oocytes needed per live born child after transfer of fresh and thawed embryos was 20 as only 5.0 % of oocytes aspirated in the first IVF/ICSI cycle had the competence to develop into a child. Conclusions In our setting, overall 5.0 % of the oocytes in a first cycle were biologically competent and in around 2/3 of all cycles, none of the oocytes had the potential to result in the birth of a child.

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Utilization of retrieved oocytes as an index of the efficiency of superovulation strategies for in-vitro fertilization treatment

Cited by 49 publications

References 0 publications

Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle

Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle

Is the presence of a non-cleaved embryo on day 3 associated with poorer quality of the remaining embryos in the cohort?

The total pregnancy potential per oocyte aspiration after assisted reproduction—in how many cycles are biologically competent oocytes available?

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