Utilization of Rosmarinic and Ascorbic Acids for Maturation Culture Media in Order to Increase Sow Oocyte Quality Prior to IVF

Abstract

The beneficial effect of antioxidant supplementation in maturation culture media of sow oocytes was evaluated by the expression quantification of apoptotic genes and the genes that ensure stability of germ cells during fertilization. The oocytes were cultivated for 44 h in conventional medium (C) or in medium supplemented with 105 µM rosmarinic acid (R) and 0.5 mM ascorbic acid (A) and classified into three quality classes by morphological observation from which the total RNA was isolated. The gene expression of Ptx3 and the apoptotic regulator p53, Bax and BCL-2 were evaluated by quantitative PCR technique. The decreased expression of the Bax gene in the A and R groups, compared to the control, indicates a protective role of antioxidants in the cells. Cell homeostasis was maintained, as reflected in the ratio of Bax/Bcl-2 in class I COCs (cumulus-oocyte complex) regardless of the experimental group, indicating minimum cellular stress. The expression of p53 genes was higher in all class III COC, but in A1 and R1 the expression was lower than in C1, and a similar Ptx-3 gene decreased significantly in groups A1, A2, A3 and R1 compared with control groups. Antioxidant supplementation showed beneficial effects on all morphological classes of pig COCs.

Multi-omics reveal the metabolic patterns in mouse cumulus cells during oocyte maturation

“…More than 40 million years ago, the inactivation of the GULO gene by a mutation left primates exclusively dependent on an exogenous dietary supply of ascorbic acid [ 49 ]. Recently, ascorbic acid has been shown to promote meiotic maturation and developmental competence in porcine oocytes [ 50 , 51 ]. The construction of carbohydrate metabolism network in cumulus cells helps us to understand the metabolic pathways derived from glycolysis and provides a mechanistic framework to perceive how ascorbic acid affects the development of COCs.…”

İnferti̇l Hastalarin Granüloza Hücreleri̇nde Li̇qui̇ri̇ti̇geni̇n’i̇n Lösemi̇ İnhi̇bi̇tör Faktör Reseptörü Üzeri̇ne Etki̇leri̇

“…domuz oositlerinin kültür olgunlaşma ortamında antioksidan takviyesinin apoptotik ve döllenme sırasında germ hücrelerinin stabilitesini sağlayan genlerin ekspresyonu üzerine etkisini değerlendirmişlerdir. Rosmarinik asit ve askorbik asit ile desteklenmiş ortamda yetiştirilen domuz oosit ve kümülüs hücre kompleksi üzerinde antioksidan takviyesinin faydalı etkiler gösterdiğini bulmuşlardır (35). Saini ve ark.…”