Utilization of soybean derivatives on clavulanic acid production by Streptomyces clavuligerus

Ortiz, S.C.A.; Hokka, Carlos O.; Badino, Alberto Colli

doi:10.1016/j.enzmictec.2006.08.009

Cited by 43 publications

(54 citation statements)

References 28 publications

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“…dd-carboxypeptidase 64-575 inhibitory activities were comparable to the levels observed for medium M cultures after fermentation in medium 11, which contained a reduced amount of Bacto™ tryptone (from 17 g to 5 g), 20 g of soybean flour and which was deprived of CSL. It was shown that soybean flour is more nutritional for bacteria in submerged cultures than other soya extracts (Ortiz et al, 2007). Overall, the conducted studies show clearly the great importance of complex nitrogen sources on production of bioactive metabolites by Streptomyces sp.…”

Section: Resultsmentioning

confidence: 73%

Characterization and Optimization of Biosynthesis of Bioactive Secondary Metabolites Produced by Streptomyces sp. 8812

Rajnisz

Guśpiel

Postek

et al. 2016

Polish Journal of Microbiology

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A b s t r a c tThe nutritional requirements and environmental conditions for a submerged culture of Streptomyces sp. 8812 were determined. Batch and fed-batch Streptomyces sp. 8812 fermentations were conducted to obtain high activity of secondary metabolites. In the study several factors were examined for their influence on the biosynthesis of the active metabolites-7-hydroxy-6-oxo-2,3,4,6-tetrahydroisoquinoline-3-carboxyl acid (C 10 H 9 NO 4 ) and N-acetyl-3,4-dihydroxy-l-phenylalanine (C 11 H 13 NO 5 ): changes in medium composition, pH of production medium, various growth phases of seed culture, amino acid supplementation and addition of anion exchange resin to the submerged culture. Biological activities of secondary metabolites were examined with the use of dd-carboxypeptidase 64-575 and horseradish peroxidase. Streptomyces sp. 8812 mycelium was evaluated under fluorescent microscopy and respiratory activity of the strain was analyzed. Moreover, the enzymatic profiles of the strain with the use of Api®ZYM test were analyzed and genetic analysis made. Phylogenetic analysis of Strepto myces sp. 8812 revealed that its closest relative is Streptomyces capoamus JCM 4734 (98%), whereas sequence analysis for 16S rRNA gene using NCBI BLAST algorithm showed 100% homology between these two strains. Biosynthetic processes, mycelium growth and enzyme inhibitory activities of these two strains were also compared.K e y w o r d s: Streptomyces sp. 8812, biologically active compounds, media optimization, submerged cultures

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Section: Resultsmentioning

confidence: 73%

Characterization and Optimization of Biosynthesis of Bioactive Secondary Metabolites Produced by Streptomyces sp. 8812

Rajnisz

Guśpiel

Postek

et al. 2016

Polish Journal of Microbiology

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“…29) Large et al 20) suggested that a reduction in residual oil levels increase the antibiotic titer. This was then experimentally demonstrated by Ortiz et al, 4) where increased CA titers were observed when the initial concentration of oils was decreased. Maranesi et al 13) also studied the effect of using vegetable oils on the production of CA in S. clavuligerus ATCC 27064.…”

Section: Discussionmentioning

confidence: 73%

“…2) Raw materials originating in soybean have been used as the nitrogen source, and starch, dextrin, glycerol, and/or oils have been used as the carbon source. [3][4][5] However, although carbohydrates, including glucose, are the simplest energy source for cell growth and secondary metabolite production, rapid catabolism of them has been shown to decrease the rate of antibiotic biosynthesis. 2) At present, lipids and oils are considered not only to be essential medium components in the antibiotic industry, due to their natural antifoam properties, but also to be cheap alternative carbon sources.…”

mentioning

confidence: 99%

An Approach to Strain Improvement and Enhanced Production of Clavulanic Acid inStreptomyces clavuligerus

Kim¹,

Kim²,

Shin³

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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“…Potassium (Ortiz et al, 2007). The fermentation was carried out in a Bioflo III model bioreactor (New Brunswick Sci.…”

Section: Source Of Camentioning

confidence: 99%

“…The cultivation was conducted batchwise at 28 °C, 800 rpm and 0.5 vvm, and the pH was automatically controlled at 6.8±0.1 by adding 2M HCl or 2M NaOH solution. Dissolved oxygen concentration was monitored by a sterilized galvanic electrode (Mettler-Toledo InPro6000 Series), (Ortiz et al, 2007;Teodoro et al, 2010).…”

Section: Source Of Camentioning

confidence: 99%

Optimization of the precipitation of clavulanic acid from fermented broth using t-octylamine as intermediate

Hirata

Oliveira

Leão

et al. 2013

Braz. J. Chem. Eng.

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-This work describes the use of clavulanic acid (CA) precipitation as the final step in the process of purification of CA from fermentation broth as an alternative to conventional methods employed traditionally. The purpose of this study was to use a stable intermediate (t-octylamine) between the conversion of CA to its salt form (potassium clavulanate), thereby enabling the resulting intermediate (amine salt of clavulanic acid) to improve the purification process and maintain the stability of the resulting potassium clavulanate. To this end, response surface methodology was employed to optimize the precipitation step. For the first reaction, five temperatures (6.6 to 23.4 °C), concentrations of clavulanic acid in organic solvent (6.6 to 23.4 mg/mL) and t-octylamine inflow rates (0.33 to 1.17 drop/min) were selected based on a central composite rotatable design (CCRD). For the second reaction, five temperatures (11.6 to 28.4 °C), concentrations of clavulanic acid amine salt in organic solvent (8.2 to 41.8 mg/mL) and concentrations of potassium 2-ethylhexanoate (0.2 to 1.2 molar) were also selected using CCRD. From these results, precipitation conditions were selected and applied to the purification of CA from the fermentation broth, obtaining a yield of 72.37%.

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Utilization of soybean derivatives on clavulanic acid production by Streptomyces clavuligerus

Cited by 43 publications

References 28 publications

Characterization and Optimization of Biosynthesis of Bioactive Secondary Metabolites Produced by Streptomyces sp. 8812

Characterization and Optimization of Biosynthesis of Bioactive Secondary Metabolites Produced by Streptomyces sp. 8812

An Approach to Strain Improvement and Enhanced Production of Clavulanic Acid inStreptomyces clavuligerus

Optimization of the precipitation of clavulanic acid from fermented broth using t-octylamine as intermediate

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