Utilizing Borassus flabellifer sprout peel sugars by Pseudomonas fluorescence for degradation of textile effluent

Abstract

The present investigation deals with the extraction of sugars from tuber peels of Borassus flabellifer and their subsequent utilization for the growth of a bacterial isolate. The study also aims to degrade the textile effluent using B. flabellifer sprout peel sugar supplemented bacterial isolate. The isolate was screened from a textile effluent and was identified as Pseudomonas fluorescence. The sugar peels were pretreated by dilute acid hydrolysis and extracted sugars were used as supplement for the growth of Pseudomonas fluorescence. The textile effluent was treated with the bacterial isolate for degradation. The decolorization and degradation was monitored using UV-Visible spectrophotometry, Fourier Transform Infrared (FT-IR) Spectroscopy and Gas Chromatography With Mass Spectrometry (GC-MS). B. flabellifer sprout peel sugar was supplemented as a macronutrient to support the growth of Pseudomonas fluorescence for the degradation of textile effluent. Higher decolorization efficiency (95%) within 7 days under aerobic condition at pH-7.0 and temperature 35˚C was achieved. The present study showed that the growth of Pseudomonas fluorescence was possible in tuber peel extracted sugars which was used as a carbon source. The bacteria grown in tuber peel extracted sugars was able to decolorize and degrade the textile effluent.