Utilizing Fcε-Bak chimeric protein for studying IgE–FcεRI interactions

Belostotsky, Ruth; Lorberboum-Galski, Haya

doi:10.1016/j.clim.2003.08.014

Cited by 11 publications

(8 citation statements)

References 34 publications

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“…DCs can be activated or inhibited through FcR by antibodies or immune complex formed by antibodies depending on the variations of FcR engaged. Activating and inhibitory IgG Fc receptors on DCs mediate opposing functions [17,18] DNA vaccination may directly target DCs involved in allergen-specific T H 1-cell responses, showing preventive effects when delivered in mice [19-23]. The vaccination with OVA-pcDNA 3.1 or OVA-Fc-pcDNA 3.1 showed preventive effects on OVA-induced hyper-production of IL-4 and IL-5 and hypo-production of INF-γ, and eosinophil infiltration, while the effects of OVA-Fc-pcDNA 3.1 were more significant.…”

Section: Discussionmentioning

confidence: 99%

Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice

Wang

Bai

Wang

et al. 2010

Genet Vaccines Ther

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BackgroundAllergen-induced imbalance of specific T regulatory (Treg) cells and T helper 2 cells plays a decisive role in the development of immune response against allergens.ObjectiveTo evaluate effects and potential mechanisms of DNA vaccine containing ovalbumin (OVA) and Fc fusion on allergic airway inflammation.MethodsBronchoalveolar lavage (BAL) levels of inflammatory mediators and leukocyte infiltration, expression of CD11c+CD80+ and CD11c+CD86+ co-stimulatory molecules in spleen dendritic cells (DCs), circulating CD4+ and CD8+ T cells, Foxp3+ in spleen CD4+ T cells and spleen CD4+ T cells were measured in OVA-sensitized and challenged animals pretreated with pcDNA, OVA-pcDNA, Fc-pcDNA, and OVA-Fc-pcDNA.ResultsOVA-Sensitized and challenged mice developed airway inflammation and Th2 responses, and decreased the proliferation of peripheral CD4+and CD8+ T cells and the number of spleen Foxp3+ Treg. Those changes with increased INF-γ production and reduced OVA-specific IgE production were protected by the pretreatment with OVA-Fc-pcDNA.ConclusionDNA vaccine encoding both Fc and OVA showed more effective than DNA vaccine encoding Fc or OVA alone, through the balance of DCs and Treg.

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Section: Discussionmentioning

confidence: 99%

Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice

Wang

Bai

Wang

et al. 2010

Genet Vaccines Ther

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“…The advantage of this strategy is that any type of IgE molecule, regardless of its specificity, can be targeted. The disadvantage is that, although the efficacy of nonanaphylactic, humanized anti-IgE antibodies in patients with asthma or peanut allergy [40][41][42] has been proven in different clinical studies, this treatment regimen will most probably be used as a concomitant therapy and not as a single treatment or as a single therapeutic option for acute allergic reactions.…”

Section: Fcgriib Puts the Brakes On Allergic Reactionsmentioning

confidence: 96%

“…The proapoptotic effect of the Fc3RI-Bak chimeric protein is directly dependent on the surface level of Fc3RI in the target cells, enabling the specific killing of Fc3RI-expressing cells [42,43]. These proapoptotic chimeric anti-Fc3RI proteins could possibly be used as a concomitant treatment of acute allergic diseases in the future.…”

Section: Fcgriib Puts the Brakes On Allergic Reactionsmentioning

confidence: 98%

“…Other approaches target Fc3RI itself, using chimeric proteins of human origin called Fc3RI-Bak-Bax, which, after internalization by mast cells and basophils, induces apoptosis of these cells [42]. The proapoptotic effect of the Fc3RI-Bak chimeric protein is directly dependent on the surface level of Fc3RI in the target cells, enabling the specific killing of Fc3RI-expressing cells [42,43].…”

Section: Fcgriib Puts the Brakes On Allergic Reactionsmentioning

confidence: 99%

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Fc receptors as determinants of allergic reactions

Kraft

Novak

2006

Trends in Immunology

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“…It was shown to bind to FcεRI-expressing cells selectively, to be slowly internalized (within hours), and to kill cells by apoptosis [47].…”

Section: Anti-fcr and Fcr Ligandsmentioning

confidence: 99%

Regulation of allergy by Fc receptors

Bruhns

Frémont

Daëron

2005

Current Opinion in Immunology

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SummaryThe aggregation of high-affinity IgE receptors (FcεRI) on mast cells and basophils has long been known to be the critical event that initiates allergic reactions. Monomeric IgE was recently found to induce a variety of effects when binding to FcεRI. Up-regulation of FcεRI required binding only, whereas other responses resulted from FcεRI aggregation. Interestingly, FcεRI aggregation has been understood to generate a mixture of positive and negative intracellular signals. Mast cells/basophils express also low-affinity and, under specific conditions, high-affinity IgG receptors. When co-engaging these receptors with FcεRI, IgG antibodies can amplify or dampen IgE-induced mast cell activation. Based on these findings, FcRs have been proposed to be used as targets and/or tools for new therapeutic approaches of allergies.

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Utilizing Fcε-Bak chimeric protein for studying IgE–FcεRI interactions

Cited by 11 publications

References 34 publications

Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice

Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice

Fc receptors as determinants of allergic reactions

Regulation of allergy by Fc receptors

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