Utilizing UV Mutagenesis to Breeding Strains with High Ability of Oil Shale Degradation

Abstract

For the screening of degrading oil shale strains, oil shale bacteria collected from Fushun Western Open were diluted moderately, then mutated with UV mutagenesis techniques, and tested the degradation. For bacteria when the mutagenic time was 1920 seconds, the death rate was 97.92%, while the degradation rate is always at 0.66% or so. For fungi, when the mutagenic time was 1920 seconds, the death rate was 93.75%, while degradability and mutagenesis time showed a certain degree of correlation. The highest degradation rate was 1.24%, when the mutagenesis time was 240s. There were three culturable fungi strains isolated from the microbial, called CPC ZYK2, ZYK4 and ZYK7. By PCR amplification of rDNA ITS area and compareing the database, the homology of the dominant strain ZYK7 and Fusarium oxysporum (JF807394) of Genbank was 100%.