UV-induced differential gene expression in rice cultivars analyzed by SSH

Abstract

The differences in gene expression between two rice genotypes-the ultraviolet-B (UV-B)-resistant cultivar Lemont and the UV-B-sensitive cultivar Dular-in response to irradiation with UV-B were studied. Suppression subtractive hybridization and real-time quantitative reverse transcriptase polymerase chain reaction were used in the analysis. The results showed that enhanced UV-B radiation could induce differential expression of the genes related to defense response. The expression of genes encoding HECT domain-containing protein, thylakoidbound ascorbate peroxidase, receptor-like protein kinase, Rad6 (ubiquitin carrier protein), phenylalanine ammonialyase, 4-coumarate: CoA ligase and chalcone synthase was up-regulated to different extents in the two rice cultivars: The cultivar Lemont exhibited a higher increase in the gene expression compared with the cultivar Dular. A large difference was found in the expression pattern of the universal stress protein (USP): showing down-regulation in Dular but up-regulation in Lemont. Hence exposure to UV-B radiation has a significant effect on gene expression in rice. In response to UV exposure, a molecular response is initially induced in different UV-B-resistant rice cultivars. This response may activate secondary metabolic pathways, in turn increase the synthesis of UV-protective substances (flavonoids) to prevent UV-B damage and induce the transcription of the relevant mRNAs for repairing the damaged DNA.

Suppression and overexpression of Lsi1 induce differential gene expression in rice under ultraviolet radiation

“…as mentioned above, seems to be vital for the development of tolerance in the rice cultivar (Fang et al 2009a). The present study certified that enhanced UV-B radiation also resulted in induced the upregulation of the gene coding for PL, which functions in splitting the cyclobutane ring and repairs the damaged DNA caused by UV (Mees et al 2004).…”

“…We used the rice accessions, Lemont (UV-B tolerant, introduced from USA) and Dular (UV-B sensitive, from India) in this study (Fang et al 2009a). The grain of the rice accession, Lemont were surface-sterilized with NaClO for 30 min, placed in Petri dishes in a temperature-controlled growth chamber and then sown in separate seedling plates.…”

“…After 7 days, each transgenic rice line and WT were exposed to enhance UV-B radiation, the treatment was followed by the method as described by Fang et al (2009a), the procedures were described as follows, fluorescent lamps (40 W, Beijing Electric Light Sources Research Institute, China) were used as the source of UV-B radiation, these lamps were suspended above the rice plants and UV-radiation of wavelength less than 280 nm (UV-C) was eliminated by wrapping the lamps in a 0.1-mm cellulose diacetate film (West Design Product Co., Ltd, United Kingdom).The distance between the rice canopy and the lamps was 30 cm and 5 lamps were used. The rice seedlings were irradiated with UV-B for a period of 1 week.…”

A Proteomic Analysis of Leaf Responses to Enhanced Ultraviolet-B Radiation in Two Rice (Oryza sativa L.) Cultivars Differing in UV Sensitivity

“…Two varieties of rice (Oryza sativa L.) were used in the experiment: Lemont (UV-B tolerant, USA) and Dular (UV-B sensitive, India) (Markham et al 1998;Fang et al 2009). …”

Expression of UV-B-induced resistant response in eggplant and control of leaf mold on greenhouse-grown eggplant.

“…．また，UV-C 照射により病害抵抗性 は誘導されるが，植物体の生育に対して激しく悪影響が出る ため (Brederode et al, 1991;Stapleton, 1992;Yalpani et al, 1994) ，主に貯蔵病害の防除に用いられている (Droby et al, 1993;Stevens et al, 1997 et al, 2003;Fujibe et al, 2000;Green et al, 1995 (Jenkins, 2009;Stratmann, 2003 et al, 2003;Fang et al, 2009;Fujibe et al, 2000;Green et al, 1995 (Brown et al, 2008;Jenkins, 2009;Stratmann, 2003…”