Uv Spectrophotometry Method Development and Validation of Sulfadiazine and Trimethoprim in Combined Dosage Form

Pokala, Ramakrishna Veni; Kumari, K. Shanta; Bollikola, Hari Babu

doi:10.22159/ijpps.2018v10i1.21767

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…Interday precision was calculated in three different days at different time intervals. The % relative standard deviation (% RSD) serves as a measure of precision [6,7].…”

Section: Precisionmentioning

confidence: 99%

Ultraviolet Spectrophotometric Method Development for Estimation of New Antiviral Repurposing Drug Favipiravir

2021

Asian J Pharm Clin Res

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Objective: A new, economical, precise, linear, sensitive, accurate, ultraviolet (UV) spectrophotometric method for the estimation of new antiviral repurposing drug favipiravir as there is no reported simple UV spectrophotometric method for estimation. The efforts were made for development and validation of favipiravir as per ICH guidelines, because drug has a wide scope for formulations to be developed for treating different viruses. Methods: This method was developed using ethanol and water as a solvent. Favipiravir showed the absorption maxima at 234 nm. A Shimadzu UV–visible spectrophotometer (UV JAPAN 1801) was used to carry out spectral analysis. Results: The developed method was linear for a range of 0–10 μg/ml and displayed a good correlation coefficient of 0.9995. Accuracy of the method was estimated using a recovery study. The amount of drug recovered was found to be in the range of 99.30–99.91%. The % relative standard deviation value of intraday precision was found to be 0.408% and interday precision was found to be in the range of 0.348–0.693%. The % relative standard deviation found to be <2 which are indicative of the precision and reproducibility of the method. Detection limit and quantitation limit were noticed as 0.095 and 0.290, respectively. Conclusion: The developed UV spectrophotometric method was validated statistically for linearity, accuracy, precision, and sensitivity and results proved that the method can be employed for routine analysis of favipiravir.

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“…Interday precision was calculated in three different days at different time intervals. The % relative standard deviation (% RSD) serves as a measure of precision [6,7].…”

Section: Precisionmentioning

confidence: 99%

Ultraviolet Spectrophotometric Method Development for Estimation of New Antiviral Repurposing Drug Favipiravir

2021

Asian J Pharm Clin Res

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“…Historically, UV spectroscopic methods were proved very beneficial for quantification purposes in pharmaceutical analytical research [8][9][10][11]. The literature search of available UV spectroscopy methods [12][13][14] showed that three UV methods were reported for estimation of PP in pharmaceutical drug products.…”

Section: Fig 1: Chemical Structure Of Paliperidone Palmitatementioning

confidence: 99%

Development and Validation of Uv Spectroscopic Method of Paliperidone Palmitate in Bulk and Its Application for Drug Loading and Entrapment Efficiency Analysis of Long-Acting Injectable Microspheres

Mali

Oza

2021

Int J App Pharm

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Objective: The objective of the current study was to develop simple, precise and cost-effective ultraviolet (UV) spectroscopic method for estimation of paliperidone palmitate (PP) as a bulk and in the polymeric depot dosage form. Methods: UV spectrophotometer with quartz sample cells and spectral manager software was used for analysis samples. 20 % Methanol in Mili-Q-Water was used as a solvent for the sample and standard preparations. Method validation was carried out as per ICH Q2 (R1) guidelines. Stress degradation studies were carried out to check the stability-indicating performance of the developed method. The validated method was applied for the estimation of the PP content of long-acting injectable microspheres of PP. Results: The wavelength of maximum absorption was found to be 283 nm. The proposed method was validated in the range of 5-30 µg/ml of PP. The mean recovery for 80%, 100% and 120% standard solution was found to be 99.03%, 99.21% and 99.35% respectively with less than 2% relative standard deviation (RSD). Intra-day and inter-day method precision ranged from 0.54 to 1.62% and 0.67 and 1.24% respectively. Limit of detection (LOD) and limit of quantitation (LOQ) were estimated to be 0.03 and 0.10 µg/ml respectively. The developed method was found to be robust and rugged. Stress degradation studies were carried out under acidic, alkaline, hydrolytic and oxidative and photo stress. The proposed method was capable to detect changes in assay due to stress conditions. Conclusion: The developed method was successfully employed for routine analysis of drug loading in long-acting injectable microspheres of PP.

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“…[2] It becomes crucial to establish a viable and dependable analytical approach for quantifying trace levels of this antibiotic in diverse matrices. [9] Several analytical procedures, including magnetic solid phase extraction (MSPE) followed by high-performance liquid chromatography, [10][11][12][13][14] HPLC, [15][16][17][18][19][20] chemiluminescence, [21] spectrometry [22] and Capillary electrophoresis-mass spectrometry, [23,24] gas chromatography [25] are used to detect SDZ. However, the electrochemical techniques [26][27][28][29][30][31][32][33] are better for detecting sulphadiazine or other drug molecules in various samples as these methods are easy and accurate.…”

Section: Introductionmentioning

confidence: 99%

Development of a Diethylcarbamazine Citrate‐Based Electrochemical Sensor for Quick and Affordable Detection of Sulfadiazine and Uric Acid in Environmental Monitoring

Teradale,

Unki,

Ganesh

et al. 2024

ChemistrySelect

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The widespread use of antibiotics like sulfadiazine (SDZ) in various industries has raised environmental and health concerns due to their potential for bioaccumulation and the subsequent effects on human health and the environment. Diethylcarbamazine citrate (DCZ), a well‐established antifilarial drug, has yet to be explored as a sensing agent despite its extensive use. This study proposes a cost‐effective and efficient method for detecting SDZ and Uric acid (UA) using a DCZ‐modified carbon paste electrode (poly‐DCZ/MCPE). The poly‐DCZ film is synthesized via cyclic voltammetry (CV) on the carbon paste electrode surface, demonstrating excellent electrocatalytic activity for SDZ and UA detection at pH 7.4. The diffusion‐controlled electrode process is observed with a lower limit of detection (LOD) and limit of quantification (LOQ) for SDZ as 3.8×10−9 M and 12.94×10−9 M respectively. For UA, LOD and LOQ were found to be 6.291×10−9 M and 20.97×10−9 M respectively at the poly‐DCZ/MCPE. Notably, the sensor exhibits simultaneous detection capabilities for SDZ and UA by CV and differential pulse voltammetry (DPV) methods, addressing the need to monitor antibiotic residues in aquatic ecosystems and animal‐derived products.

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Uv Spectrophotometry Method Development and Validation of Sulfadiazine and Trimethoprim in Combined Dosage Form

Cited by 6 publications

References 21 publications

Ultraviolet Spectrophotometric Method Development for Estimation of New Antiviral Repurposing Drug Favipiravir

Ultraviolet Spectrophotometric Method Development for Estimation of New Antiviral Repurposing Drug Favipiravir

Development and Validation of Uv Spectroscopic Method of Paliperidone Palmitate in Bulk and Its Application for Drug Loading and Entrapment Efficiency Analysis of Long-Acting Injectable Microspheres

Development of a Diethylcarbamazine Citrate‐Based Electrochemical Sensor for Quick and Affordable Detection of Sulfadiazine and Uric Acid in Environmental Monitoring

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