Uvaol Improves the Functioning of Fibroblasts and Endothelial Cells and Accelerates the Healing of Cutaneous Wounds in Mice

Abstract

Uvaol is a natural pentacyclic triterpene that is widely found in olives and virgin olive oil, exerting various pharmacological properties. However, information remains limited about how it affects fibroblasts and endothelial cells in events associated with wound healing. Here, we report the effect of uvaol in the in vitro and in vivo healing process. We show the positive effects of uvaol on migration of fibroblasts and endothelial cells in the scratch assay. Protein synthesis of fibronectin and laminin (but not collagen type I) was improved in uvaol-treated fibroblasts. In comparison, tube formation by endothelial cells was enhanced after uvaol treatment. Mechanistically, the effects of uvaol on cell migration involved the PKA and p38-MAPK signaling pathway in endothelial cells but not in fibroblasts. Thus, the uvaol-induced migratory response was dependent on the PKA pathway. Finally, topical treatment with uvaol caused wounds to close faster than in the control treatment using experimental cutaneous wounds model in mice. In conclusion, uvaol positively affects the behavior of fibroblasts and endothelial cells, potentially promoting cutaneous healing.

Sterols and Triterpene Diols in Virgin Olive Oil: A Comprehensive Review on Their Properties and Significance, with a Special Emphasis on the Influence of Variety and Ripening Degree

“…Erythrodiol exhibited antiproliferative and apoptotic effects against adenocarcinoma [44] and promoted the lowering of LDL-cholesterol levels in human blood [45]. Uvaol has been effective in promoting accelerated and improved wound healing [46] and alleviating inflammatory bowel disease in mice [47], while in humans, it has shown antitumor activity [27,48].…”

Advances in Research on Chemical Constituents and Their Biological Activities of the Genus Actinidia

“…Callus tissue from the stems of A. arguta (Actinidiaceae) produced three ursane-type triterpenes including ursolaldehyde 41, α-amyrin 42, and uvaol 43 [26]. Of them, compound 43 showed anti-inflammatory, anticancer, and wound healing activities [27][28][29]. Antiinflammatory properties of 43 on DSS-induced colitis and LPS-stimulated macrophages have been explored detailly and completely.…”

“…arguta [ 34 ] Leaves 39 (2 α ,3 β )-2,3,23-Trihydroxyurs-13(18)-en-28-oic acid A. chinensis Planch [ 52 ] Roots 40 Fupenzic acid A . chinensis [ 25 ] Root bark Antiviral (in vitro) [ 25 ] 41 Nrsolaldehyde A. arguta [ 26 ] Stems 42 α -Amyrin A. arguta [ 26 ] Stems 43 Uvaol A. arguta [ 26 ] Stems Anti-inflammatory (in vivo) [ 27 ] Anticancer (in vitro) [ 28 ] Wound healing (in vivo) [ 29 ] 44 Pseudotaraxasterol A. chinensis Planch [ 19 ] Roots 45 2 α ,3 α ,24-Trihydroxyurs-11-en-13,28-olide A. polygama [ 23 ] Fruit galls 46 2 α ,3 β -Dihydroxyurs-12-en-28,30-olide A. chinensis [ 30 ] Roots …”

Collagen Peptides Derived from Sipunculus nudus Accelerate Wound Healing

“…A number of drugs and health care products that promote wound healing are mainly designed according to the four stages on which intervene, such as hemostatic drugs, antimicrobial drugs, anti-inflammatory drugs or agents that promote collagen synthesis. Recent research demonstrated that the decrease of inflammation and highly orchestrated remodeling could improve the performance of new regenerative skin, and even achieve scarless healing [ 5 , 6 ], and many Chinese herbal medicine and marine bioactivity substances, such as collagen (peptides), have been shown to accelerate skin wound healing and prevent abnormal scar formation [ 7 , 8 , 9 ].…”