UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells

Li, Wei; Wu, Di; Hua, Lijuan; Zhou, Bingrong; Guo, Zhendong; Luo, Dan

doi:10.1016/s1674-8301(11)60017-1

Cited by 18 publications

(12 citation statements)

References 31 publications

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“…Previous studies have revealed that UVB irradiation could induce the alteration of miRNAs expression in HaCaT cells, such as the up-regulation of miR-139-5p, 30 miR-1246, 31 miR-23a, 32 and miR-141. 33 Meanwhile, the aberrant expression levels of miRNAs are frequently closely linked with cellular processes including apoptosis and inflammatory responses in UVB-irradiated HaCaT cells through modulating downstream genes or signaling cascades. For example, Li et al 31 reported that miR-1246 overexpression down-regulated the expression of RTKN2 through directly binding to its 3′-UTR, thus enhancing cell apoptosis induced by UVB irradiation in HaCaT cells.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED: MicroRNA-145 attenuates IL-6-induced enhancements of sensitivity to UVB irradiation by suppressing MyD88 in HaCaT cells

Dong

Jiang

Chen

et al. 2018

Int J Immunopathol Pharmacol

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MicroRNAs (miRNAs/miRs) play vital roles in various immune diseases including systemic lupus erythematosus (SLE). The current study aimed to assess the role of miR-145 in interleukin-6 (IL-6)-treated HaCaT cells under ultraviolet B (UVB) irradiation and further explore the potential regulatory mechanism. HaCaT cells were pretreated with IL-6 and then exposed to UVB to assess the effect of IL-6 on sensitivity of HaCaT cells to UVB irradiation. The levels of miR-145 and MyD88 were altered by transfection and the transfected efficiency was verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR)/western blot analysis. Cell viability, percentage of apoptotic cells and expression levels of apoptosis-related factors were measured by trypan blue assay, flow cytometry assay, and western blot analysis, respectively. In addition, the levels of c-Jun N-terminal kinases (JNK) and nuclear factor-κB (NF-κB) signaling pathway-related factors were assessed by western blot analysis. IL-6 treatments significantly aggravated the reduction of cell viability and promotion of cell apoptosis caused by UVB irradiation in HaCaT cells. Interestingly, miR-145 level was augmented by UVB exposure and miR-145 mimic alleviated IL-6-induced increase of sensitivity to UVB irradiation in HaCaT cells, as dramatically increased cell viability and reduced cell apoptosis. Opposite effects were observed in miR-145 inhibitor-transfected cells. Meanwhile, MyD88 was negatively regulated by miR-145 and MyD88 mediated the regulatory effect of miR-145 on IL-6- and UVB-treated cells. In addition, miR-145 mimic inhibited the JNK and NF-κB pathways by down-regulating MyD88. In conclusion, the present study demonstrated that miR-145 alleviated IL-6-induced increase of sensitivity to UVB irradiation by down-regulating MyD88 in HaCaT cells.

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Section: Discussionmentioning

confidence: 99%

RETRACTED: MicroRNA-145 attenuates IL-6-induced enhancements of sensitivity to UVB irradiation by suppressing MyD88 in HaCaT cells

Dong

Jiang

Chen

et al. 2018

Int J Immunopathol Pharmacol

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“…The miRNA, miR-23a, regulates DNA damage repair and apoptosis by targeting topoisomerase-1, caspase-7 and serine/ threonine kinase 4 (STK4) (17). In addition, UVB radiation induces apoptosis by upregulating miR-141, which then leads to the suppression of phosphatase and tensin homolog (PTEN) in keratinocytes (18). Aberrantly expressed miRNAs have been linked to apoptosis and cell cycle arrest in UVB-exposed…”

Section: Introductionmentioning

confidence: 99%

Troxerutin induces protective effects against ultraviolet B radiation through the alteration of microRNA expression in human HaCaT keratinocyte cells

Lee¹,

Jun

Lee³

et al. 2014

International Journal of Molecular Medicine

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Ultraviolet light B (UVB), contained in sunlight, induces damaging effects on skin by impairing cells in the epidermis and dermis. In particular, keratinocytes in the epidermis are those cells which are mainly affected by UVB light. UVB radiation induces cell death, growth arrest, DNA damage and restricts cell migration. Various phytochemicals have been shown to alleviate UVB-induced cellular damage. Troxerutin is a natural flavonoid rutin mainly found in extracts of Sophora japonica, and is a well-known antioxidant and anti-inflammatory compound used in experimental mouse models. In this study, we examined the effects of troxerutin on UVB-induced damage in HaCaT cells. HaCaT cells were pre-treated with troxerutin (0-10 µM) and then exposed to UVB radiation (50 mJ/cm2). Cell viability, cell cycle and migration assays were performed to determine the protective effects of troxerutin on the cells. DNA repair activity was also measured. Troxerutin protected the cells against UVB-induced damage, such as cell death, growth arrest, restriction of cell migration and decreased DNA repair activity in HaCaT cells. Analyses of microRNA (miRNA) expression demonstrated that the protective effects of troxerutin correlated with alterations in miRNA expression, as indicated by Gene Ontology analyses of putative target genes. Overall, our data demonstrate that troxerutin exerts protective effects against UVB-induced damage by regulating miRNA expression.

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“…To elucidate the molecular mechanisms underlying photodamage, especially skin carcinogenesis by UVB, several research groups have investigated miRNA expression profiles in UVB irradiated cells using miRNA microarrays[4]-[7]. Guo et al [4].…”

Section: Introductionmentioning

confidence: 99%

Baicalin modulates microRNA expression in UVB irradiated mouse skin

Zhou

et al. 2012

Journal of Biomedical Research

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This study aimed to evaluate the effects of baicalin on ultraviolet radiation B (UVB)-mediated microRNA (miRNA) expression in mouse skin. We determined miRNA expression profiles in UVB irradiated mice, baicalin treated irradiated mice, and untreated mice, and conducted TargetScan and Gene Ontology analyses to predict miRNA targets. Three miRNAs (mmu-miR-125a-5p, mmu-miR-146a, and mmu-miR-141) were downregulated and another three (mmu-miR-188-5p, mmu-miR-223 and mmu-miR-22) were upregulated in UVB irradiated mice compared with untreated mice. Additionally, these miRNAs were predicted to be related to photocarcinogenesis, hypomethylation and apoptosis. Three miRNAs (mmu-miR-378, mmu-miR-199a-3p and mmu-miR-181b) were downregulated and one (mmu-miR-23a) was upregulated in baicalin treated mice compared with UVB irradiated mice, and they were predicted to be related to DNA repair signaling pathway. These deregulated miRNAs are potentially involved in the pathogenesis of photodamage, and may aid treatment and prevention of UVB-induced dermatoses.

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UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells

Cited by 18 publications

References 31 publications

RETRACTED: MicroRNA-145 attenuates IL-6-induced enhancements of sensitivity to UVB irradiation by suppressing MyD88 in HaCaT cells

RETRACTED: MicroRNA-145 attenuates IL-6-induced enhancements of sensitivity to UVB irradiation by suppressing MyD88 in HaCaT cells

Troxerutin induces protective effects against ultraviolet B radiation through the alteration of microRNA expression in human HaCaT keratinocyte cells

Baicalin modulates microRNA expression in UVB irradiated mouse skin

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