UW is Superior Compared with HTK After Prolonged Preservation of Renal Grafts

Hoeger, Simone; Lueg, Georgia; Tsagogiorgas, Charalambos; Schneider, Michael; Theisinger, Sonja; Theisinger, Bastian; Fontana, Johann; Waldherr, R; Krämer, Bernhard K.; Schnuelle, Peter; Yard, Benito A.

doi:10.1016/j.jss.2011.05.020

Cited by 19 publications

(8 citation statements)

References 48 publications

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“…Several other studies have documented renal dysfunction following various times of CS, although many are much longer than 4 hr [4,15,16]. Our study revealed, for the first time, that CS (4 hr) leads to a selective respiratory complex inhibition (I and III, but not II and IV) and ATP depletion following transplantation.…”

Section: Resultssupporting

confidence: 60%

“…While advances in tissue-type matching and immunosuppressive protocols have greatly reduced short-term graft dysfunction after renal transplantation, long-term graft function continues to be problematic, especially in patients receiving deceased donor kidneys [1][2][3]. The kidneys of deceased donors are routinely flushed and stored in cold storage (CS) solutions [4]. Short-term CS lowers the metabolic demand, yet, prolonged CS causes extensive tissue damage and is associated with a higher incidence of delayed graft function and poor long-term outcome after transplant [1,5,6].…”

Section: Introductionmentioning

confidence: 99%

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Cold Storage Exacerbates Renal and Mitochondrial Dysfunction Following Transplantation

Shrum

Parajuli

2016

J Kidney

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Long-term renal function is compromised in patients receiving deceased donor kidneys which require cold storage exposure prior to transplantation. It is well established that extended cold storage induces renal damage and several labs, including our own, have demonstrated renal mitochondrial damage after cold storage alone. However, to our knowledge, few studies have assessed renal and mitochondrial function after transplantation of rat kidneys exposed to short-term (4 hr) cold storage compared to transplant without cold storage (autotransplantation). Our data reveal that cold storage plus transplantation exacerbated renal and mitochondrial dysfunction when compared to autotransplantation alone.Citation: Shrum S, MacMillan-Crow LA, Parajuli N (2016) Cold Storage Exacerbates Renal and Mitochondrial Dysfunction Following Transplantation.

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Section: Resultssupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Cold Storage Exacerbates Renal and Mitochondrial Dysfunction Following Transplantation

Shrum

Parajuli

2016

J Kidney

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“…Optimisation of conditions during cold ischemia time is therefore essential. Cold storage solutions were designed to increase organ tolerance and preserve cellular integrity during ischemia [ 40 , 41 ]. Reducing the temperature of the kidney to 4 °C reduces enzyme activity, decreases oxygen requirement and lowers metabolism by 58 % [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

Improving the outcome of kidney transplantation by ameliorating renal ischemia reperfusion injury: lost in translation?

et al. 2016

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Kidney transplantation is the treatment of choice in patients with end stage renal disease. During kidney transplantation ischemia reperfusion injury (IRI) occurs, which is a risk factor for acute kidney injury, delayed graft function and acute and chronic rejection. Kidneys from living donors show a superior short- and long-term graft survival compared with deceased donors. However, the shortage of donor kidneys has resulted in expansion of the donor pool by using not only living- and brain death donors but also kidneys from donation after circulatory death and from extended criteria donors. These grafts are associated with an increased sensitivity to IRI and decreased graft outcome due to prolonged ischemia and donor comorbidity. Therefore, preventing or ameliorating IRI may improve graft survival. Animal experiments focus on understanding the mechanism behind IRI and try to find methods to minimize IRI either before, during or after ischemia. This review evaluates the different experimental strategies that have been investigated to prevent or ameliorate renal IRI. In addition, we review the current state of translation to the clinical setting. Experimental research has contributed to the development of strategies to prevent or ameliorate IRI, but promising results in animal studies have not yet been successfully translated to clinical use.

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“…Moreover, UWS can minimize hypothermic-induced cell swelling, prevent interstitial edema and intracellular acidosis, scavenge oxygen-free radicals, and provide substrates for regenerating high-energy phosphate compounds (Belzer, Southard, 1988). The solution is the gold standard for graft preservation and has been successfully used to preserve explanted liver, heart, and kidney prior to transplantation (Lopez-Andujar et al, 2009;George et al, 2011;Hoeger et al, 2011). Thus, we chose UWS as nerve graft preservation solution in this experiment.…”

Section: Discussionmentioning

confidence: 99%

Tetramethylpyrazine protects Schwann cells from ischemia-like injury and increases cell survival in cold ischemic rat nerves

Yang

Huang

Jiang

2015

Braz. J. Pharm. Sci.

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Tetramethylpyrazine (TMP), a major active ingredient of Ligusticum wallichi Franchat extract (a Chinese herb), exhibits neuroprotective properties in ischemia. In this study, we assessed its protective effects on Schwann cells (SCs) by culturing them in the presence of oxygen glucose deprivation (OGD) conditions and measuring cell survival in cold ischemic rat nerves. In the OGD-induced ischemic injury model of SCs, we demonstrated that TMP treatment not only reduced OGD-induced cell viability losses, cell death, and apoptosis of SCs in a dose-dependent manner, and inhibited LDH release, but also suppressed OGD-induced downregulation of Bcl-2 and upregulation of Bax and caspase-3, as well as inhibited the consequent activation of caspase-3. In the cold ischemic nerve model, we found that prolonged cold ischemic exposure for four weeks was markedly associated with the absence of SCs, a decrease in cell viability, and apoptosis in preserved nerve segments incubated in University of Wisconsin solution (UWS) alone. However, TMP attenuated nerve segment damage by preserving SCs and antagonizing the decrease in nerve fiber viability and increase in TUNEL-positive cells in a dose-dependent manner. Collectively, our results indicate that TMP not only provides protective effects in an ischemia-like injury model of cultured rat SCs by regulating Bcl-2, Bax, and caspase-3, but also increases cell survival and suppresses apoptosis in the cold ischemic nerve model after prolonged ischemic exposure for four weeks. Therefore, TMP may be a novel and effective therapeutic strategy for preventing peripheral nervous system ischemic diseases and improving peripheral nerve storage. Uniterms:Tetramethylpyrazine. Schwann cell. Oxygen glucose/deprivation. Peripheral nerve. Ischemia/ experimental study. University of Wisconsin solution.Tetrametilpirazina (TMP), o principal componente do extrato de Ligusticum wallichi Franchat (erva chinesa), apresenta propriedades neuroprotetoras na isquemia. Nesse estudo, avaliamos seus efeitos protetores nas células de Schwann (SC), cultivando-as na presença de condições de depleção de oxigênio da glicose (OGD) e medindo a sobrevivência dos nervos de ratos isquêmicos pelo resfriamento. No modelo de lesão isquêmica em SC induzida por OGD, demonstramos que o tratamento com TMP não somente reduziu as perdas de viabilidade celular induzida por OGD, a morte celular, a apoptose de SC dose-dependente e inibiu a liberação de LDH, mas, também, suprimiu a infra-regulação do Vcl-2 e a supra-regulação de Bax e caspase-3, e inibiu a consequente ativação da caspase-3. No modelo de nervo isquêmico por resfriamento, observamos que a exposição prolongada ao resfriamento por quatro semanas estava, marcadamente, associada com a ausência de SC, com o decréscimo da viabilidade celular e a apoptose em segmentos de nervo incubados na solução da Universidade de Wisconsin apenas. Entretanto, a TMP atenuou o dano no segmento do nervo preservando SC e antagonizando a diminuição da viabilidade da fibra nervosa e o aumento das cé...

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UW is Superior Compared with HTK After Prolonged Preservation of Renal Grafts

Cited by 19 publications

References 48 publications

Cold Storage Exacerbates Renal and Mitochondrial Dysfunction Following Transplantation

Cold Storage Exacerbates Renal and Mitochondrial Dysfunction Following Transplantation

Improving the outcome of kidney transplantation by ameliorating renal ischemia reperfusion injury: lost in translation?

Tetramethylpyrazine protects Schwann cells from ischemia-like injury and increases cell survival in cold ischemic rat nerves

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