v-jun encodes a nuclear protein with enhancer binding properties of AP-1

Bos, Timothy J.; Bohmann, Dirk; Tsuchie, Hideaki; Tijan, Robert; Vogt, Peter K.

doi:10.1016/0092-8674(88)90408-4

Cited by 188 publications

(126 citation statements)

References 14 publications

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“…The single Gag-related protein present at low levels in normal CEF is probably derived from an endogenous chicken virus. Discrepancies between calculated and apparent molecular weights due to abnormal electrophoretic properties of Myc, Jun and Fos proteins are in agreement with previous observations (Bos et al, 1988;Hartl et al, 1991Nishizawa et al, 1987;Patschinsky et al, 1986). Interestingly, chemically transformed QT6 cells infected with ASV17 and expressing the Gag ± Jun hybrid protein still contained a reduced level of endogenous c-Jun protein, whereas in QEF transformed by oncogenic jun or myc alleles expression of the corresponding endogenous proteins is completely suppressed (Gao et al, 1996;Penn et al, 1990).…”

Section: Bkj Activation In ®Broblasts Is Also Induced By V-fossupporting

confidence: 91%

“…QEF transformed by the RCAS ± CJ3 or RCAS ± VJ1 constructs contained high levels of retrovirally speci®ed c-Jun (328 amino acids, M r 35 880, apparent molecular weight 42 000) or v-Jun (295 amino acids, M r 32 314, apparent molecular weight 36 000) proteins ( Figure 2A). Both ASV17-transformed QEF and ASV17-infected QT6 cells contained high levels of the Gag ± Jun hybrid protein (516 amino acids, M r 54 943, apparent molecular weight 65 000) (Figure 2A) speci®ed by ASV17 (Bos et al, 1988). QEF transformed by RCAS ± MC29 contained high levels of the Gag ± Myc hybrid protein (875 amino acids, M r 94 259, apparent molecular weight 110 000) speci®ed by MC29 (Bister et al, 1977).…”

Section: Bkj Activation In ®Broblasts Is Also Induced By V-fosmentioning

confidence: 99%

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Structure and transcriptional regulation of BKJ, a novel AP-1 target gene activated during jun- or fos-induced fibroblast transformation

Hartl

Bister

1998

Oncogene

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The BKJ gene was originally identi®ed based on its speci®c transcriptional activation in jun-transformed avian ®broblasts. We now show that BKJ is a direct transcriptional target of the AP-1 transcription factor components Jun and Fos. The complete structural organization of the quail BKJ gene was determined by nucleotide sequence analysis and transcriptional mapping. The gene contains three exons with the coding region con®ned to the third exon. A major mRNA species of 0.8 kb and a minor one of 1.3 kb are produced by variable usage of two transcriptional initiation sites. The BKJ promoter region contains two authentic AP-1 binding sites. By transactivation of reporter gene constructs and direct binding of Jun recombinant protein, the proximal AP-1 element was shown to be essential for BKJ promoter activation. Using polyclonal antiserum directed against recombinant BKJ protein, the activation of BKJ in jun-transformed avian ®broblasts was also demonstrated at the protein level. BKJ is a novel gene related to the avian b-keratin gene family whose members display highly speci®c expression patterns during embryogenesis and epidermal development. Activation of BKJ in ®broblasts by retroviral or deregulated cellular jun or fos alleles may contribute to cell transformation.

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Section: Bkj Activation In ®Broblasts Is Also Induced By V-fossupporting

confidence: 91%

Section: Bkj Activation In ®Broblasts Is Also Induced By V-fosmentioning

confidence: 99%

Structure and transcriptional regulation of BKJ, a novel AP-1 target gene activated during jun- or fos-induced fibroblast transformation

Hartl

Bister

1998

Oncogene

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“…The Jun protein was originally identified in transformed cells carrying the genome of a replication defective avian sarcoma virus 17 (ASV 17) that directs the expression of a 65-kDa gag-jun fusion product, designated as v-Jun (Maki et al, 1987;Bos et al, 1988). Soon after, its homolog, the proto-oncogene c-Jun, was isolated from human and murine tissues (Bohmann et al, 1987;.…”

Section: C-jun a Member Of The Ap-1 Complexmentioning

confidence: 99%

c-Jun, at the crossroad of the signaling network

2011

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“…V-Fos was isolated from retroviruses that cause osteosarcomas in mice (Curran and Teich, 1982) and are able to transform murine fibroblasts and keratinocytes in vitro Lee et al, 1993). V-jun was originally isolated from a retrovirus that causes sarcomas in chickens, and transforms chick embryo fibroblasts (Bos et al, 1988). V-Fos transformed 208F rat fibroblasts express an elongated bipolar morphology and are capable of anchorage-independent growth, but are still dependent upon mitogens for proliferation, suggesting a separation of the morphological from the proliferative aspects of transformation in this system (Hawker et al, 1993;Miao and Curran, 1994).…”

Section: Introductionmentioning

confidence: 99%