Vaccarin protects human microvascular endothelial cells from apoptosis via attenuation of HDAC1 and oxidative stress

Zhu, Xiaofang; Lei, Yueyue; Tan, Fanggen; Gong, Leilei; Gong, Haifeng; Yang, Wei; Chen, Ting; Zhang, Zhixuan; Cai, Weiwei; Hou, Bao; Wang, Xu; Sun, Hai-Jian; Zhou, Yuetao; Qiu, Ling

doi:10.1016/j.ejphar.2017.09.052

Cited by 23 publications

(15 citation statements)

References 43 publications

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“…Several studies have used this Ang II dose-range to induce inflammatory and oxidative responses [ 36 , 37 , 38 , 39 , 40 ]. On the other hand, HMEC-1 cells were chosen as a model because they are employed in various studies to induce endothelial dysfunction, increasing the production of ROS and inflammatory factors [ 41 , 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Cucumis sativus Aqueous Fraction Inhibits Angiotensin II-Induced Inflammation and Oxidative Stress In Vitro

et al. 2018

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Inflammation and oxidative stress play major roles in endothelial dysfunction, and are key factors in the progression of cardiovascular diseases. The aim of this study was to evaluate in vitro the effect of three subfractions (SFs) from the Cucumis sativus aqueous fraction to reduce inflammatory factors and oxidative stress induced by angiotensin II (Ang II) in human microvascular endothelial cells-1 (HMEC-1) cells. The cells were cultured with different concentrations of Ang II and 0.08 or 10 μg/mL of SF1, SF2, or SF3, or 10 μmol of losartan as a control. IL-6 (Interleukin 6) concentration was quantified. To identify the most effective SF combinations, HMEC-1 cells were cultured as described above in the presence of four combinations of SF1 and SF3. Then, the effects of the most effective combination on the expression of adhesion molecules, the production of reactive oxygen species (ROS), and the bioavailability of nitric oxide (NO) were evaluated. Finally, a mass spectrometry analysis was performed. Both SF1 and SF3 subfractions decreased the induction of IL-6 by Ang II, and C4 (SF1 and SF3, 10 μg/mL each) was the most effective combination to inhibit the production of IL-6. Additionally, C4 prevented the expression of adhesion molecules, reduced the production of ROS, and increased the bioavailability of NO. Glycine, arginine, asparagine, lysine, and aspartic acid were the main components of both subfractions. These results demonstrate that C4 has anti-inflammatory and antioxidant effects.

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Section: Discussionmentioning

confidence: 99%

Cucumis sativus Aqueous Fraction Inhibits Angiotensin II-Induced Inflammation and Oxidative Stress In Vitro

et al. 2018

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“…Cells were plated at a density of 3 × 10 3 per well in a 96-well microplate and incubated at 37 °C overnight. The cells were treated with various concentrations of test compounds (the final concentrations of the compounds were 0, 5, 10, 20, and 40 µM) for 48 h. Then, cell viability was determined by CCK-8 assay [19,20].…”

Section: Methodsmentioning

confidence: 99%

Chemical Constituents from Albiziae Cortex and Their Ability to Ameliorate Steatosis and Promote Proliferation and Anti-Oxidation In Vitro

Shi

Cai

et al. 2019

Molecules

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This study describes the chemical constituents of Albiziae Cortex and their ability to ameliorate steatosis and promote proliferation and anti-oxidation in vitro. Together, five known lignan glycosides, (7S,8R)-erythro-syringylglycerol-β–O-4′-sinapyl ether 9-O-β-D-glucopyranoside (1), (+)-lyoniresinol-9′-O-gluco-side (2), (−)-lyoniresinol-9′-O-glucoside (3), picraquassioside C (4), and icariside E5 (5), were isolated from the Albiziae Cortex. Their structures were elucidated by extensive NMR and high-resolution mass spectrometry analysis and compared with reported data. Oil Red O staining results revealed that compounds 1, 2, and 3 attenuated lipid accumulation and lipid metabolic disorders in FFAs (oleate/palmitate, 2:1 ratio, 0.3 mM)-exposed HepG2 cells. The Cell Counting Kit 8 (CCK-8) assay results revealed that compounds 1 and 5 can significantly promote human umbilical vein endothelial cell (HUVEC) proliferation; meanwhile, these compounds did not exhibit significant cytotoxicity against HUVECs. In addition, 2‘,7‘-dichlorofluorescein diacetate (DCFH-DA) staining results revealed that high glucose (HG)-induced reactive oxygen species (ROS) production was abolished by compounds 1, 2, and 3. This is the first report of the isolation of lignan skeletons from the genus Albizzia julibrissin with the ability to ameliorate steatosis and promote proliferation and anti-oxidation activities.

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“…The total protein was extracted by RIPA lysis buffer (Cwbio, Nanjing, China) with protease and phosphatase inhibitor (Cwbio, Nanjing, China) as peer-described [9] and quantified with BCA protein assay kit (Beyotime Biotechnology, Shanghai, China). Equal amounts of protein were electrophoresed, blotted, and incubated with the required antibodies at 4 • C overnight.…”

Section: Western Blottingmentioning

confidence: 99%

“…In vitro experiments revealed that VAC promoted proliferation of human microvascular endothelial cells (HMEC-1) by activating the fibroblast growth factor-2 (FGF-2) mediated fibroblast growth factor receptor (FGFR-1) signaling pathway under normal conditions [7]. Recently, it has been found that VAC could promote NO production via inhibition of the ROS/AMPK/miRNA-34a/eNOS signaling cascade [8], and protect HG-induced endothelial cell apoptosis by inhibiting the accumulation of reactive oxygen species and the expression of histone deacetylase 1 (HDAC1) [9]. In addition, bacterial cellulose-vaccarin (BC-VAC) membranes not only enhanced the mechanical and physical properties of the cellulose membrane but also promoted acute mechanical wound healing [10].…”

Section: Introductionmentioning

confidence: 99%

Vaccarin Regulates Diabetic Chronic Wound Healing through FOXP2/AGGF1 Pathways

Liu

Sun

et al. 2020

IJMS

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Background: Diabetes mellitus is a growing global health issue nearly across the world. Diabetic patients who are prone to develop diabetes-related complications often exhibit progressive neuropathy (painless and sensory loss). It is usual for small wounds to progress to ulceration, which especially worsens with peripheral arterial disease and in the presence of anaerobic bacteria, culminating into gangrene. In our study, vaccarin (VAC), the main active monomer extracted from Chinese herb vaccariae semen, is proven to have a role in promoting diabetic chronic wound healing through a cytoprotective role under high glucose conditions. Materials and methods: We constructed a pressure ulcer on both VAC-treated and control mice based on a type 1 diabetes (T1DM) model. The wound healing index was evaluated by an experimental wound assessment tool (EWAT). We also determined the effect of VAC on the proliferation and cell migration of human microvascular endothelial cells (HMEC-1) by a cell counting kit (CCK-8), a scratch and transwell assay. Results: The results demonstrated that VAC could promote the proliferation and migration of high glucose-stimulated HMEC-1 cells, which depend on the activation of FOXP2/AGGF1. Activation of the angiogenic factor with G patch and FHA domains 1 (AGGF1) caused enhanced phosphorylation of serine/threonine kinase (Akt) and extracellular regulated protein kinases (Erk1/2). By silencing the expression of forkhead box p2 (FOXP2) protein by siRNA, both mRNA and protein expression of AGGF1 were downregulated, leading to a decreased proliferation and migration of HMEC-1 cells. In addition, a diabetic chronic wound model in vivo unveiled that VAC had a positive effect on chronic wound healing, which involved the activation of the above-mentioned pathways. Conclusions: In summary, our study found that VAC promoted chronic wound healing in T1DM mice by activating the FOXP2/AGGF1 pathway, indicating that VAC may be a promising candidate for the treatment of the chronic wounds of diabetic patients.

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Vaccarin protects human microvascular endothelial cells from apoptosis via attenuation of HDAC1 and oxidative stress

Cited by 23 publications

References 43 publications

Cucumis sativus Aqueous Fraction Inhibits Angiotensin II-Induced Inflammation and Oxidative Stress In Vitro

Cucumis sativus Aqueous Fraction Inhibits Angiotensin II-Induced Inflammation and Oxidative Stress In Vitro

Chemical Constituents from Albiziae Cortex and Their Ability to Ameliorate Steatosis and Promote Proliferation and Anti-Oxidation In Vitro

Vaccarin Regulates Diabetic Chronic Wound Healing through FOXP2/AGGF1 Pathways

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