Vacuolation in the human amnion cell studied by time-lapse photography and electron microscopy

Armstrong, W. D.; Wilt, James; Pritchard, E.T.

doi:10.1016/0002-9378(68)90452-3

Cited by 29 publications

(10 citation statements)

References 19 publications

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“…Although no inflammatory infiltrate was noted in the amniotic membranes exposed to meconium in our series, we observed an increased vacuolization of the cytoplasm of the amniotic cells as was previously noted by Bartman and Blanc [9]. The significance of this phenomenon remains unclear [3,71], but is probably related to the rapid turnover of some processes such as absorption, transmission and secretion which are characterized of some diseases [3,9], Another hypothesis investigated in these series of experiments was the acidification theory of the amniotic fluid. According to G raff [30] and Ikeda [35], the acidification of the amniotic fluid is held responsible for the morphological picture of a granulocytic infiltration of the chorion and by extrapolation also of the pulmonary parenchyma.…”

Section: Discussionsupporting

confidence: 67%

Intrauterine Pneumonia

Lauweryns¹,

Bernat²,

Lerut³

et al. 1973

Neonatology

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Several agents usually supposed from experiments done on adult animals and during extrauterine life to induce a ‘congenital pneumonia’, have been directly introduced into the amniotic cavity of rabbit fetuses and their effects studied after 72 hours. Human meconium, rabbit amniotic cellular debris, gastric juice or acidified amniotic fluid did not produce any granulocytic inflammatory reaction within the lungs or the fetal membranes. The injection of various types of bacteria revealed on microscopic examination an acute chorioamnionitis and usually also the peculiarities (intrapulmonary polymorphs and amniotic fluid cells; no fibrin; no septicemia) of an ‘intrauterine pneumonia’, resembling the congenital pneumonia’ of the human infant. The umbilical cord and other fetal mucous membranes disclosed no inflammatory lesions. These experiments argue in favour of an exclusive bacterial origin in the induction of an intrauterine pneumonia.

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Section: Discussionsupporting

confidence: 67%

Intrauterine Pneumonia

Lauweryns¹,

Bernat²,

Lerut³

et al. 1973

Neonatology

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“…She suggested that these two cells represented distinct cell populations and that they performed different functions in the human amniotic epithelium. While some other researchers also supported this view (Armstrong et al 1968;Wynn and French 1968;McCoshen et al 1981), others rejected this possibility (Lister 1968;Sinha 1971;Hoyes 1972) or made no mention of the cell types (Sonek et al 1991;McLaren et al 1999). All these previous studies, however, employed only conventional chemical (glutaraldehyde) fixation followed by simple TEM observation, and did not use other morphological methodologies.…”

Section: Discussionmentioning

confidence: 99%

“…She suggested that these epithelial cells consisted of heterogeneous cell populations, solely from the ultrastructural viewpoint. Since then, the heterogeneity of the amniotic epithelial cells has long been argued by several researchers: some agreed (Armstrong et al 1968;Wynn and French 1968;McCoshen et al 1981) while others denied this possibility (Lister 1968;Sinha 1971;Hoyes 1972;Sonek et al 1991). To date, the heterogeneity/homogeneity of the amniotic epithelial cells has not yet been determined.…”

mentioning

confidence: 99%

Human amniotic epithelial cells are morphologically homogeneous: enzymehistochemical, tracer, and freeze-substitution fixation study

Iwasaki

Matsubara²,

Tajima³

et al. 2009

Eur J Histochem

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We examined the fine subcellular morphology of human amniotic epithelial cells and attempted to answer the question as to whether amniotic epithelial cells consist of heterogeneous or homogeneous cells, which has long been controversial. Study subjects were fetal membranes from pregnant women (n=18) who abdominally gave birth to healthy infants at term (37.9±0.7 weeks of gestation, mean±sd). The methods employed were transmission electron microscopy, enzymehistochemistry, tracer permeability analysis, and freeze-substitution fixation. The labelings for acid phosphatase, cytochrome c oxidase, and CA ++ ATPase were seen in the lysosomes, mitochondria, and lateral plasma membranes, respectively. The staining distribution pattern of these three enzymes and the morphology of the organelle highlighted by these enzymehistochemistry did not differ among cells. Freeze-substitution fixation revealed that intercellular spaces in the amniotic epithelial cells were narrower than previously thought, but the tracers (horse radish peroxidase and lanthanum nitrate) fully entered these spaces. There were no variations in the tracer permeability among cells. All cells from freeze-substitution fixation exhibited the same morphological features. From these morphological viewpoints, we conclude that human term amniotic epithelial cells consist of a homogeneous cell population.

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“…In the sheep model of chronic severe placental insufficiency, the amount of amniotic fluid decreases without changes in the urine volume, suggesting that the amniotic fluid volume decreases due to the increased absorption of amniotic fluid from membranes . Amniotic epithelial cells have been reported to express aquaporin and may play an important role in regulating the amniotic fluid volume . Thus, it is possible that disorders of amniotic epithelial cells caused by decomposition products of blood cells induce the abnormal distribution of amniotic fluid or occult rupture of membranes, causing oligohydramnios.…”

Section: Discussionmentioning

confidence: 99%

“…12 Amniotic epithelial cells have been reported to express aquaporin and may play an important role in regulating the amniotic fluid volume. 13,14 Thus, it is possible that disorders of amniotic epithelial cells caused by decomposition products of blood cells induce the abnormal distribution of amniotic fluid or occult rupture of membranes, causing oligohydramnios. There are reports of amniotic necrosis by meconium, and the relationship between meconium staining of membranes and reduction of the amniotic fluid volume, 14 suggesting a correlation between amniotic necrosis and amniotic fluid volume abnormalities.…”

Section: Discussionmentioning

confidence: 99%

Amniotic epithelial cells damage by oxidative stress in cases of diffuse chorioamniotic hemosiderosis

Iizuka

Ono

Masumoto

et al. 2019

J of Obstet and Gynaecol

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The amniotic membrane plays an important role in the physiological maintenance and protection of the embryo. Indeed, dysfunction of the amniotic membrane is thought to have an adverse effect on the continuation of pregnancy. In this report, we examined the pathological changes in the amniotic epithelium in three cases of diffuse chorioamniotic hemosiderosis (DCH) and investigated the cause of necrosis of the amniotic epithelial cells and its relationship with oligohydramnios. Diffuse chorioamniotic hemosiderosis was confirmed in all three cases. More extensive amniotic epithelial necrosis led to more severe hemosiderosis. Immunostaining for 8‐hydroxy‐2′‐deoxyguanosine, a marker of oxidative stress, was positive in the amniotic epithelial cells. We speculate that oxidative DNA damage of the amniotic epithelium occurs by decomposition products of blood cells in cases accompanying subchorionic hematomas and pathological DCH. Furthermore, disorder of the amniotic epithelium may disrupt the balance of the amniotic fluid volume and cause oligohydramnios.

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Vacuolation in the human amnion cell studied by time-lapse photography and electron microscopy

Cited by 29 publications

References 19 publications

Intrauterine Pneumonia

Intrauterine Pneumonia

Human amniotic epithelial cells are morphologically homogeneous: enzymehistochemical, tracer, and freeze-substitution fixation study

Amniotic epithelial cells damage by oxidative stress in cases of diffuse chorioamniotic hemosiderosis

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