Vacuole and mitochondria patch (vCLAMP) and ER-mitochondria encounter structure (ERMES) maintain cell survival by protecting mitochondrial functions in Candida albicans

Mao, Xiaolong; Peng, Liping; Yang, Li; Zhu, Mengsen; Duan, Huan‐Feng; Ma, Congcong; Yu, Qilin; Li, Mingchun

doi:10.1016/j.bbrc.2021.12.116

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…While MCU is lacking, the mutual interplay between different organelles also occurs in yeast cells ( 79 ). For the interaction between ER and mitochondria, the ER-mitochondria encounter structure (ERMES) has been identified in Saccharomyces cerevisiae and C. albicans ( 80 ). The ERMES complex is involved in organelle tethering and transfer of calcium ions and lipids ( 81 ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial peptide LL-37 disrupts plasma membrane and calcium homeostasis in Candida albicans via the Rim101 pathway

Chen,

Chang,

Lan

2023

Microbiol Spectr

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Antimicrobial peptides play an important role in human innate defense against microbial pathogens. LL-37 is the only member of the human cathelicidin family of antimicrobial peptides. We previously showed that LL-37 targets the cell wall of Candida albicans , thereby reducing cell adhesion to plastic surfaces, oral epidermoid cells, and urinary bladders of mice. Moreover, LL-37 also induces cell wall and endoplasmic reticulum stress responses. Here, we found that LL-37 alters plasma membrane properties. In addition, LL-37 affected calcium homeostasis and induced the generation of reactive oxygen species associated with mitochondria and vacuoles. Finally, the Rim101 pathway was linked to the cellular response to LL-37. Together, these findings provide new insights into anti- C . albicans actions of LL-37 and highlight the complex cellular responses induced by LL-37. IMPORTANCE Candida albicans is a major human fungal pathogen, and antimicrobial peptides are key components of innate immunity. Studying the interplay between C. albicans and human antimicrobial peptides would enhance a better understanding of pathogen-host interactions. Moreover, potential applications of antimicrobial peptides in antifungal therapy have aroused great interest. This work explores new mechanisms of LL-37 against C. albicans and reveals the complex connection among calcium homeostasis, oxidative stress, signaling, and possibly organelle interaction. Notably, these findings support the possible use of antimicrobial peptides to prevent and treat fungal infections.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial peptide LL-37 disrupts plasma membrane and calcium homeostasis in Candida albicans via the Rim101 pathway

Chen,

Chang,

Lan

2023

Microbiol Spectr

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The vacuolar fusion regulated by HOPS complex promotes hyphal initiation and penetration in Candida albicans

Liu,

Wang,

Liu

et al. 2024

Nat Commun

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The transition between yeast and hyphae is crucial for regulating the commensalism and pathogenicity in Candida albicans. The mechanisms that affect the invasion of hyphae in solid media, whose deficiency is more related to the pathogenicity of C. albicans, have not been elucidated. Here, we found that the disruption of VAM6 or VPS41 which are components of the homotypic vacuolar fusion and protein sorting (HOPS) complex, or the Rab GTPase YPT72, all responsible for vacuole fusion, led to defects in hyphal growth in both liquid and solid media, but more pronounced on solid agar. The phenotypes of vac8Δ/Δ and GTR1OE-vam6Δ/Δ mutants indicated that these deficiencies are mainly caused by the reduced mechanical forces that drive agar and organs penetration, and confirmed that large vacuoles are required for hyphal mechanical penetration. In summary, our study revealed that large vacuoles generated by vacuolar fusion support hyphal penetration and provided a perspective to refocus attention on the role of solid agar in evaluating C. albicans invasion.

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Better Together: Interorganellar Communication in the Regulation of Proteostasis

Kohler,

Kohler

2024

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An extensive network of chaperones and folding factors is responsible for maintaining a functional proteome, which is the basis for cellular life. The underlying proteostatic mechanisms are not isolated within organelles, rather they are connected over organellar borders via signalling processes or direct association via contact sites. This review aims to provide a conceptual understanding of proteostatic mechanisms across organelle borders, not focussing on individual organelles. This discussion highlights the precision of these finely tuned systems, emphasising the complicated balance between cellular protection and adaptation to stress. In this review, we discuss widely accepted aspects while shedding light on newly discovered perspectives.

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Vacuole and mitochondria patch (vCLAMP) and ER-mitochondria encounter structure (ERMES) maintain cell survival by protecting mitochondrial functions in Candida albicans

Cited by 3 publications

References 25 publications

Antimicrobial peptide LL-37 disrupts plasma membrane and calcium homeostasis in Candida albicans via the Rim101 pathway

Antimicrobial peptide LL-37 disrupts plasma membrane and calcium homeostasis in Candida albicans via the Rim101 pathway

The vacuolar fusion regulated by HOPS complex promotes hyphal initiation and penetration in Candida albicans

Better Together: Interorganellar Communication in the Regulation of Proteostasis

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