2023
DOI: 10.1093/jee/toad160
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Vacuum extraction: an effective larval sampling method for spotted-wing drosophila in small fruit crops

Arun Babu,
Rosan Adhikari,
Ashfaq A Sial

Abstract: Detecting and sampling the pest for pest management, either through enumerating their life stages or by quantifying the crop damage, is the cornerstone in deploying integrated pest management. Currently, for spotted-wing drosophila, Drosophila suzukii Matsumura, larval extraction from the fruit samples involves immersing the fruits in hot water, salt, or sugar solution. We are introducing a novel, fast, and effective larval sampling technique where D. suzukii larvae can be extracted from infested fruits by sub… Show more

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Cited by 3 publications
(4 citation statements)
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“…Alternative methods based either on fruit dunk floatation and filtration or vacuum extraction are characterized by larval recoveries lower than 100% as some larvae can remain within the fruit (Babu et al 2023). This results in the underestimation of the intensity of infestation and a lower accuracy than with the sleeve method.…”
Section: Discussionmentioning
confidence: 99%
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“…Alternative methods based either on fruit dunk floatation and filtration or vacuum extraction are characterized by larval recoveries lower than 100% as some larvae can remain within the fruit (Babu et al 2023). This results in the underestimation of the intensity of infestation and a lower accuracy than with the sleeve method.…”
Section: Discussionmentioning
confidence: 99%
“…For alternative methods, total processing time per fruit is typically 120 seconds or higher (Van Timmeren et al 2021), which likely results in a lower throughput than the sleeve method. Finally, other methods require a liquid either for dunk floatation (Van Timmeren et al 2021) or for rinsing fruits to collect the remaining larvae (Babu et al 2023), which cannot be reused.…”
Section: Discussionmentioning
confidence: 99%
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