Valepotriate content in different in vitro cultures of Valerianaceae and characterization of Valeriana officinalis L. callus during a growth period

Abstract

Different in vitro cultures of Valerianaceae were analysed for valepotriate content [(iso)valtrate, acevaltrate, didrovaltrate] in a study on properties of production in vitro (plant species, growth conditions, differentiation level, valepotriate content of the medium after growth). The in vitro cultures were: callus cultures of Valeriana officinalis L., Valerianella locusta L. and Centranthus ruber L.DC.; a suspension culture of Valeriana officinalis L. and a root organ culture of Centranthus ruber L.DC. All of the cultures produced valepotriates in vitro in different amounts. None of the media that had served for growth contained any valepotriates. In order to characterize the in vitro growth more precisely different parameters were analysed at different time intervals during a growth period in one of the cultures (callus culture of Valeriana officinalis L.). These different parameters were: fresh and dry weight, lipid and nitrogen content and (iso)valtrate content. This study during a growth period was performed on two media differing in plant hormone content.

Production of essential oils and flavours in plant cell and tissue cultures. A review

“…The valepotriate content of different Valerianaceae tissue cultures was studied [21,22,122,159,160,161]. The yield of valepotriates and the relative amounts of the components varied from species to species.…”

“…Since no valepotriates were detected in the culture media, it was concluded that these compounds were accumulated intracellularly [159]. The influence of artificially-induced higher ploidy levels on the valepotriate production by suspension cultures of Valeriana wallichii DC.…”

“…Violon and co-workers [159] reported that addition of 2,4-D to the culture medium stimulated the growth and primary metabolism of callus cultures of Valeriana officinalis, but diminished the production of valepotriates. Contrary to these results Chavadej [40] suggested that the synthesis of these secondary metabolites by suspension cultures of V. wallichii could be stimulated by adding an increased concentration of 2,4-D and coconut milk to the growth medium.…”

“…Also Violon and co-workers [159] reported that light was not an indispensable factor for valepotriate production in callus cultures of V. offieinalis.…”

Valerian (Valeriana officinalisL.)

“…The composition of the essential oil of valerian depends on the chemovar, and the most common compounds include bornyl acetate, valerenal, valeranone, and cryptofauronol. 19 Minor constituents of the essential oil include α -pinene, β -pinene, camphene, β -caryophyllene, terpinolene, limonene, and carvyl acetate.…”