Validación de una técnica de Dot blot para la detección de Cercospora kikuchii en plantas de soja

Abstract

Cercospora kikuchii is a common pathogen in soybean plants that causes crop spoilage. Its early and precise identification would prevent the misuse of pesticides and allow the initiation of an appropriate treatment. A quick, economical and easy-to-execute technique is the Dot blot, capable of recognizing the presence of a genus-specific protein called CFP (Cercosporin Facilitator Protein). The objective was to validate this technique to guarantee the reliability of the results. For that purpose, 29 infected soybean plants and 31 healthy plants were processed, taking into account a 95% desired confidence level and a permissible error of 5%. The technique provided a diagnostic sensitivity of 93.3% and a diagnostic specificity of 96.7%. The efficiency was 95% and positive and negative predictive values were 96.6% and 93.5%, respectively. These results postulate it as a useful resource for the early detection of C. kikuchii in soybean plants.

Biochemical Characterization of a Novel α/β-Hydrolase/FSH from the White Shrimp Litopenaeus vannamei

“…The polyclonal antibodies should be specific against the antigen for which they were prepared and show selectivity and reproducibility [57,58]. Polyclonal antibodies (pAb), representing a more varied collection of paratopes for the same antigen, provided more probabilities of detection.…”

“…An important validation when working with pAbs is cross-reactivity [57,58]. It was found that the anti-LvLIP antibodies did not show cross-reactivity and no detection was shown in the TBS controls.…”