Validated HPLC Determination of the Potential Anti-Helicobacter pylori, Lepidine, in Lepidium sativum Seeds Assessed by Molecular Docking Study

Abstract

Background: Nearly 50 % of the world residents are known to be infected with Helicobacter pylori which is the main cause of peptic ulcer and gastric cancer. Since resistance to the currently used treatments, other alternative approaches such as combination with natural products should be tried. Objectives: In this work, validated HPLC-UV method was developed for quantification of lepidine content in various extracts and crude oil of Lepidium sativum seeds. Furthermore, antibacterial activity of lepidine against H. pylori was compared with standard antibiotics supported by molecular docking study. Methods: Different solvents were used to extract lepidine as well as the contents were determined using HPLC-UV method. Anti-Helicobacter pylori activity was examined using agar diffusion and dilution methods. Molecular docking study was done on H. pylori phosphoribosyltransferase enzyme. Results: The highest content of lepidine (316.91 µg mL-1) was found in n-hexane extract. Lepidine exhibit antibacterial effect (MIC = 6.25 µg mL-1) against H. pylori clinical isolates. The ability of lepidine to interact with amino acids in the phosphoribosyl transferase binding site might rationalize its observed activity. Conclusion: Our results demonstrate for the first time the anti-Helicobacter pylori activity of lepidine, which could therefore be developed as viable nutraceutical agent. Further investigations are required to formulate suitable pharmaceuticals to combat with the H. pylori infections on clinical grounds.

The metabolomic analysis of five Mentha species: cytotoxicity, anti-Helicobacter assessment, and the development of polymeric micelles for enhancing the anti-Helicobacter activity

“…Broth microdilution was performed with 96-well U-shaped bottom microtiter plates following Clinical and Laboratory Standards Institute (CLSI) guidelines (2017). 38,39 …”

“…Broth microdilution was performed with 96-well Ushaped bottom microtiter plates following Clinical and Laboratory Standards Institute (CLSI) guidelines (2017). 38,39 The tested samples were used in an initial concentration of 100 mg mL À1 and then serially diluted two-fold to obtain concentrations in the range of 0.098-50 mg mL À1 . The inoculum of H. pylori was prepared in Columbia broth, with the OD 600 nm adjusted to 0.5.…”

Underivatized Amino Acid Chromatographic Separation: Optimized Conditions for HPLC-UV Simultaneous Quantification of Isoleucine, Leucine, Lysine, Threonine, Histidine, Valine, Methionine, Phenylalanine, Tryptophan, and Tyrosine in Dietary Supplements

“…The obtained results indicated that satisfactory accuracy and precision were obtained as the values of RSD did not exceed 2%, with additional mean recoveries within the range of 98–102%. 38 , 39 The method’s robustness was estimated by investigating the deliberate change of flow rate and acetonitrile percentile by 0.2 units. The mentioned changes did not remarkably affect the results, indicating the satisfactory performance of the method for implementation in different laboratories.…”