Validated spectrofluorimetric method for determination of mirabegron by utilizing its quenching effect on acetoxymercuric fluorescein reagent

Morshedy, Samir; Omran, Gamal A.; Abdullatef, O. A.; Omar, Mahmoud A.; Talaat, Wael

doi:10.1016/j.saa.2020.118509

Cited by 15 publications

(11 citation statements)

References 13 publications

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“…3) [9], this reaction decreases the intensity of the (AMF)fluorescence that measured quantitatively with the tested compounds [10][11][12]. Many compounds successfully determined quantitively using this method such as mesna, acetylcysteine, timonacic corrosive [13], penicillamine [14] and mirabegron [15]. In this study, the reaction of IXB with its sulfide group with AMF and the quenching effect on the fluorescence were measured spectrofluorimetricaly at (ƛ em 530 nm) [9].…”

Section: Ixabepilonementioning

confidence: 95%

A Profoundly Touchy Approved Spectrofluorimetric Approach for Assurance of Ixabepilone as Anticancer Drug by Utilizing Its Quenching Impact on Acetoxymercuric Fluorescein Reagent

Salem

Abdelaziz

Gamal

et al. 2021

IRJPAC

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A dependable, sensitive, basic and cheap spectrofluorimetric approach has been created for test of sulfur-containing drug; ixabepilone in bulk powder, vials and human plasma. The approach depends on the quenching effect of ixabepilone on the fluorescence intensity of acetoxymercuric fluorescene (AMF) reagent at λem of 530 nm and λex of 500 nm. Parameters which will control the reaction such as pH, AMF solution concentration, temperature, time and solvents were examined and optimized. According to the optimized conditions, the proposed approach was practiced over the concentration area of 20-100 ng mL-1 with adequate linearity (r = 0.9998). The developed approach was approved confirming to ICH rules in terms of accuracy, precision, linearity, LOD and LOQ. The proposed approach was practiced to analyze ixabepilone in Ixempra® vials with satisfactory recovery % of 99.89 and RSE% of 1.24. The results achieved were compared to those achieved by an already reported HPLC approach.

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Section: Ixabepilonementioning

confidence: 95%

A Profoundly Touchy Approved Spectrofluorimetric Approach for Assurance of Ixabepilone as Anticancer Drug by Utilizing Its Quenching Impact on Acetoxymercuric Fluorescein Reagent

Salem

Abdelaziz

Gamal

et al. 2021

IRJPAC

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“…Literature review showed different methods for the determination of MIR using UV spectrophotometric [3] , spectrofluorometric [4] , chromatographic methods [5–7] LC‐MS/MS [8,9] .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Determination of Mirabegron at Silica Modified Carbon Paste in Micellar Medium: Sensitive Application with Drug Product and Spiked Plasma

2022

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Sensitive, simple, and accurate differential pulse voltammetric (DPV) method was validated and used to estimate mirabegron (MIR) at silica modified carbon paste sensor (Si‐CPE) in micellar media. The electrochemical nature of MIR revealed irreversible diffusion‐controlled oxidation. Various experimental conditions were optimized, under optimum conditions, good linearity was obtained within range 1.25×10−7−6.25×10−5 M with correlation coefficient 0.9997. The suggested method was efficiently performed for estimating MIR in a pharmaceutical dosage form and spiked human plasma with high precise recoveries (%) ±RSD 98.60±0.88 %, 99.10±0.54 %, respectively. The suggested method could be used as a stability‐indicating assay to assess MIR with its degradation product without any interference.

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“…The research was conducted on the Blend Uniformity (BU) stage, with finished product stage formulation samples, and developed a quantification method for MBN. Several authors have reported several methods for MBN quantification by spectrophotometric estimation (Morshedy et al, 2020; Papanna et al, 2018; Rao et al, 2017; Roopa et al, 2021). Few authors have reported a UV spectrophotometric method for bulk drug analysis (Ravisankar et al, 2016; Suresh et al, 2020) or LC/MS and thin‐layer chromatography methods for clinical analysis (Shah et al, 2019; Van Teijlingen et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Unique liquid chromatography technique for the determination of mirabegron in the presence of polymers; robustness by Design Expert in the light of Quality by Design

Konduru¹,

Kowtharapu

Gundla

2022

Biomedical Chromatography

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The current study is designed to estimate mirabegron in the presence of high molecular weight polymers using a unique liquid chromatography method and sample preparation technique. The proposed method is significant because of the many analytical issues faced during the development studies. Based on the experimental results, we finally achieved the stability-indicating power of the method. The adequately prepared mobile phase was in the ratio of pH 2.0 buffer and acetonitrile (80:20) v/v, and the buffer pH 2.0 was prepared as follows: 8.7 ml of perchloric acid, 2 ml of triethylamine and 3.0 g sodium hydroxide into 1 L of water mixed well. The system suitability parameters were achieved using a Waters X-Bridge C 18 (4.6 Â 150 mm, 3.5 μm) column and mobile phase. The optimized chromatographic conditions included a column temperature of 45 C, a flow rate of 1.0 ml min À1 ; an injection volume of 5 μl, UV 247 nm, and 15 min runtime. The method was validated and transferred to quality control as per International Conference on Harmonization Q2(R1) and the Chinese Pharmacopoeia 2020 edition <9101> and <9100>. The recovery and linearity results were obtained between 99.0 and 101.0%; the value of r 2 was 0.9998. The method robustness study was established by utilizing the Design of Experiments part of the Quality by Design concept. The method's stability-indicating nature was proved by a forced degradation study; all of the conditions for analyte peak purity were passed, and mass balance was achieved. The method was used to determine mirabegron assay, as well as content uniformity, blend uniformity and cleaning samples. It is a user-friendly and cost-effective method.

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Validated spectrofluorimetric method for determination of mirabegron by utilizing its quenching effect on acetoxymercuric fluorescein reagent

Cited by 15 publications

References 13 publications

A Profoundly Touchy Approved Spectrofluorimetric Approach for Assurance of Ixabepilone as Anticancer Drug by Utilizing Its Quenching Impact on Acetoxymercuric Fluorescein Reagent

A Profoundly Touchy Approved Spectrofluorimetric Approach for Assurance of Ixabepilone as Anticancer Drug by Utilizing Its Quenching Impact on Acetoxymercuric Fluorescein Reagent

Electrochemical Determination of Mirabegron at Silica Modified Carbon Paste in Micellar Medium: Sensitive Application with Drug Product and Spiked Plasma

Unique liquid chromatography technique for the determination of mirabegron in the presence of polymers; robustness by Design Expert in the light of Quality by Design

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