Validated Spectrophotometric Methods for Determination of Weakly UV absorbed Perindopril Arginine in Bulk and Combined Dosage Form

El‐Bagary, Ramzia I.; Mowaka, Shereen; Elkady, Ehab F.; Attallah, Maria A.

doi:10.1080/22297928.2016.1250673

Cited by 7 publications

(3 citation statements)

References 19 publications

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“…8 is substituted in Eq.5 to determine the peak amplitude of Y in the mixture at λ1 Py in the mixture = a+b(conc. of y) .…………….9 Where Pmix1 absorbance of mixture of [X (PRG) + Y (E2)] at λ 1 Pmix2: absorbance of mixture of [X (PRG) + Y (E2)] at λ 2 Where Py in the mixture: absorbance of y (E2) in the mixture, y: concentration of y (E2) Subtracting calculate concentration of Y in Eq.8 by Pmix1 Pmix1-(Py in the mix) = P𝑥 in the mixture ……..10 Where Pmix1: absorbance of mixture of [X (PRG) + Y (E2)] at λ 1, Py in the mixture: absorbance of y (E2) in the mixture and P𝑥 in the mixture: absorbance of X (PRG) in the mixture By subsuming Eq.10 in Eq.7 the concentration of drug X (PRG) can be determined P𝑥 in the mixture = a+b𝑥 ……………11 Where P𝑥: absorbance of X (PRG) in the mixture, a: absorptivity, b: slop, X: concentration of X (PRG) in the mixture 25 .…”

Section: Modified Amplitude Subtraction Methods (Mas)mentioning

confidence: 99%

Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods

2023

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Four rapid, accurate and very simple derivative spectrophotometric techniques were developed for the quantitative determination of binary mixtures of estradiol (E2) and progesterone (PRG) formulated as a capsule. Method I is the first derivative zero-crossing technique, derivative amplitudes were detected at the zero-crossing wavelength of 239.27 and 292.51 nm for the quantification of estradiol and 249.19 nm for Progesterone. Method II is ratio subtraction, progesterone was determined at λmax 240 nm after subtraction of interference exerted by estradiol. Method III is modified amplitude subtraction, which was established using derivative spectroscopy and mathematical manipulations. Method IIII is the absorbance ratio technique, absorbance of both medicines was measured at two wavelengths λ1= 260, -absorptive point and λ2=240max of progesterone. The Q equations were used to calculate the final concentrations. The calibration curve is linear from 5.0–140 and 2.0–32.0 µg/ml for estradiol and progesterone respectively. The proposed techniques' selectivity was tested using synthetic combinations created in the lab and assessed using the standard addition method. Using one-way ANOVA, the outputs of the proposed ways were compared, and the result showed no significant differences between the proposed techniques.

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Section: Modified Amplitude Subtraction Methods (Mas)mentioning

confidence: 99%

Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods

2023

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“…Perindopril (PER) is an antihypertensive nonsulfhydryl angiotensin‐converting enzyme (ACE) inhibitor that is used to treat hypertension and stable coronary artery disease with the nomenclature of (2 S ,3 aS ,7 aS )‐1‐[(2 S )‐2‐[[(2 S )‐1‐ethoxy‐1‐oxopentan‐2‐yl]amino]propanoyl]‐2,3,3 a ,4,5,6,7,7 a ‐octahydroindole‐2‐carboxylic acid (Figure 1b). For determining PER alone or in combination, various analytical procedures have been published including spectrophotometry [3, 4, 7, 8, 43–45], spectrofluorimetry [46–49], and chromatographic approaches including TLC [30], LC–MS/MS [31] and HPLC [4, 34–36, 39, 50, 51].…”

Section: Introductionmentioning

confidence: 99%

“…For determining PER alone or in combination, various analytical procedures have been published including spectrophotometry [3,4,7,8,[43][44][45], spectrofluorimetry [46][47][48][49], and chromatographic approaches including TLC [30], LC-MS/MS [31] and HPLC [4, 34-36, 39, 50, 51].…”

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confidence: 99%

Utilization of erythrosine B in spectrofluorimetric quenching analysis of amlodipine and perindopril in pharmaceutical and biological matrices

et al. 2023

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A spectrofluorimetric approach that is sensitive, simple, validated, and cost‐effective has been proposed for the estimation of amlodipine (AML) and perindopril (PER) in their bulk powders, pharmaceutical formulations, and spiked human plasma. The recommended approach utilized the quantitative quenching effect of the two cited drugs on the fluorescence intensity of erythrosine B, as a result of complex binary reactions among each drug with erythrosine B at pH 3.5 (Teorell and Stenhagen buffer). The quenching of erythrosine B fluorescence was recorded at 554 nm after excitation at 527 nm. The calibration curve was detected in the range 0.25–3.0 μg ml−1, with a correlation coefficient of 0.9996 for AML, and 0.1–1.5 μg ml−1, with a correlation coefficient of 0.9996 for PER. The established spectrofluorimetric approach was validated for the estimation of the cited drugs with high sensitivity regarding International Council on Harmonization guidelines. Therefore, the established approach could be utilized for quality control of the cited drugs in their pharmaceutical formulations.

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Spectrofluorimetric assay of amlodipine and perindopril in their raw materials, pharmaceutical formulations and spiked human plasma through the formation of complexes with Eosin Y

et al. 2023

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For quantitation of amlodipine (AML) and perindopril (PER) in their authentic, pharmaceutical formulations and spiked human plasma, a simple, sensitive, validated and inexpensive spectrofluorimetric method has been developed. The proposed method is developed to be based on quantitative quenching effect of two antihypertensive drugs on Eosin Y's native fluorescence which was achieved by developing binary complexes between each of the cited drugs in an acidic environment using acetate buffer (pH 4.4) with Eosin Y. Fluorescence quenching was recorded at 544 nm after excitation at 425 nm. For AML and PER, calibration curves were obtained over the range of 0.3–3.0 µg/mL and 0.2–2.0 µg/mL, respectively, with correlation coefficients of 0.9993 and 0.9995, respectively. The developed method was validated according to ICH guidelines. The proposed spectrofluorimetric method is regarded new and sensitive. As a result, the proposed method might be used to estimate the quality of the cited drugs in their pharmaceutical formulations and biological fluid.

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Validated Spectrophotometric Methods for Determination of Weakly UV absorbed Perindopril Arginine in Bulk and Combined Dosage Form

Cited by 7 publications

References 19 publications

Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods

Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods

Utilization of erythrosine B in spectrofluorimetric quenching analysis of amlodipine and perindopril in pharmaceutical and biological matrices

Spectrofluorimetric assay of amlodipine and perindopril in their raw materials, pharmaceutical formulations and spiked human plasma through the formation of complexes with Eosin Y

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