Validation and application of an UPLC–MS/MS method for the quantification of synthetic cannabinoids in urine samples and analysis of seized materials from the Portuguese market

Simões, Susana Sadler; Silva, Inês; Ajenjo, Antonio Castañera; Dias, Mário

doi:10.1016/j.forsciint.2014.07.022

Cited by 41 publications

(31 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, determination of the intake of synthetic cannabinoids largely depends on metabolite detection. So far, several approaches for detection of synthetic cannabinoid metabolites in urine have been published, and recently presented nontargeted approaches enabled reduction of the time and effort required for detecting newly synthesized drugs [18,20,21]. High-resolution quadrupole time-of-flight mass spectrometry (QTOF-MS) screening methods have been developed as qualitative analytical techniques, facilitating easy update for new derivatives [22,23].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of 37 synthetic cannabinoid metabolites in human urine by liquid chromatography-tandem mass spectrometry

et al. 2015

View full text Add to dashboard Cite

Despite efforts by legal authorities to control the abuse of synthetic cannabinoids, new derivatives have continually emerged on the market to circumvent regulations, and its abuse has become a threat to public health. Thus, development of analytical methods for confirming drug intake in biological fluids is essential to ensure effective drug control and to address further drug intoxication cases. Herein, a sensitive and reliable liquid chromatography-tandem mass spectrometry method was established and validated for the simultaneous determination of 37 synthetic cannabinoid metabolites, such as N-hydroxypentyl and carboxy metabolites, using 100 ll of urine. Urine specimens were treated by enzymatic hydrolysis and solid-phase extraction. Limits of detection for the evaluated drugs ranged from 0.1 to 1 ng/ml, and the linear range spanned from 0.25 or 1 to 100 ng/ml. Precision and accuracy bias were 1.4-12.1 % and -7.2-7.2 %, respectively. Matrix effects biases were in the range of 0.4 to 10.1 %, and extraction recoveries were 65-99 %. In addition, all analytes were stable under storage conditions of 4°C and -20°C for 14 days, and after three freeze-thaw cycles. The developed method was successfully applied to actual urine specimens obtained from synthetic cannabinoid users. The present method enabled simultaneous quantification of 37 synthetic cannabinoid metabolites, including their regioisomers, in urine in the field of clinical and forensic toxicology.

show abstract

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of 37 synthetic cannabinoid metabolites in human urine by liquid chromatography-tandem mass spectrometry

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The retention time was 21.54 min. A UPLC-MS/MS method with gradient elution was developed in another study [19]. The time was 1 h for enzymatic hydrolysis.…”

Section: Confirmation Of the Cedia (+) Results Of The Real Samples Wimentioning

confidence: 99%

“…There are methods developed with sample preparation techniques like liquid-liquid extraction [17], solid-phase extraction [5,18,19] used for THC-COOH determination in the literature, with low detection and quantification limits. Most of them have relatively long sample preparation or chromatographic analysis times.…”

Section: Introductionmentioning

confidence: 99%

Fast confirmation for marijuana metabolite: THC-COOH, ultra-fast LC—MS/MS run time, and application to routine samples

Zazoğlu

Anılanmert

Aydın

et al. 2017

Acta Chromatographica

View full text Add to dashboard Cite

Summary.A fast, reliable, inexpensive, and practical method with a low determination limit and high recovery has been developed for the determination of the marijuana metabolite in routine analysis. THC-COOH in urine was validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Before an easy single-step extraction with Toxi-Tubes, basic hydrolysis was performed at 60 °C for 30 min. LC-MS/MS analysis takes 2.5 min for each sample, and the retention time of the analyte is 1.75 min. Specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, repeatability, and intermediate precision (inter-day) system suitability parameters were determined in the validation study. The recovery of the extraction method was 88.67 (±5.91). LOD and LOQ values were 1.41 and 5.00 ng mL −1 , respectively. The method showed linear response between the values 5.00 and 500.00 ng mL −1 . The repeatability was 9.64% (relative standard deviation, RSD%), and the intermediate precisions (RSD R %) were 10.73%, 13.74%, and 8.11% at 10.00, 100.00, and 200.00 ng mL −1 concentration levels, respectively. No statistically significant difference was found in ANOVA analysis, between three consecutive days in intermediate precision study, for 90% confidence level. HorRat values were between 0.34 and 0.61. The method was applied to CEDIA positive samples, obtained from the Trabzon Group Presidency of Turkish Council of Forensic Medicine, successfully.

show abstract

“…Extraction of SCs using SPE has been used in blood and serum [91], urine [92][93][94][95][96] and oral fluid [45,97,98]. Different types of SPE columns can be used depending on the cost, availability and the nature of the analytes of interest.…”

Section: Solid Phase Extractionmentioning

confidence: 99%

Bioanalytical methods for the determination of synthetic cannabinoids and metabolites in biological specimens

Aldlgan

Torrance

2016

TrAC Trends in Analytical Chemistry

View full text Add to dashboard Cite

Recently the use of synthetic cannabinoids (SCs) has increased around the world. As a result, the importance for accurate analysis of SCs in human biological matrices is evident and continues to be especially challenging due to their chemical structures being constantly modified. Many methods have been published recently for the analysis of SCs in human biological samples. This review provides an overview of the analytical methods used for the analysis of SCs and their metabolites in biological specimens with a special focus on chromatographic analysis and sample preparation.Liquid chromatography assay is the most commonly used for confirmation purposes of SCs and their metabolites in biological matrices. In blood and oral fluid, analysis of SCs must be very sensitive. In urine, SCs have extensive metabolism pathways; therefore the main target compounds are their hydroxyl and carboxyl metabolites, which is important to recognise when establishing clinical and forensic toxicology analytical methods.

show abstract

Validation and application of an UPLC–MS/MS method for the quantification of synthetic cannabinoids in urine samples and analysis of seized materials from the Portuguese market

Cited by 41 publications

References 49 publications

Simultaneous quantification of 37 synthetic cannabinoid metabolites in human urine by liquid chromatography-tandem mass spectrometry

Simultaneous quantification of 37 synthetic cannabinoid metabolites in human urine by liquid chromatography-tandem mass spectrometry

Fast confirmation for marijuana metabolite: THC-COOH, ultra-fast LC—MS/MS run time, and application to routine samples

Bioanalytical methods for the determination of synthetic cannabinoids and metabolites in biological specimens

Contact Info

Product

Resources

About