Validation of a 16-Locus Fluorescent Multiplex System

Krenke, Benjamin E.; Tereba, Allan; Anderson, Steven E.; Buel, Eric; Culhane, Sherry; Finis, Carla J.; Tomsey, Christine S.; Zachetti, Jeffrey M.; Masibay, Arni S.; Rabbach, Dawn R.; Amiott, Elizabeth A.; Sprecher, Cynthia J.

doi:10.1520/jfs15445j

Cited by 263 publications

(155 citation statements)

References 9 publications

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“…The cycling conditions were 1 cycle of 2 min at 95°C, 45 cycles of 30 s at 94°C, 1 min at 58°C, 1 min at 72°C and a final extension of 50 min at 60°C. Simplex reactions targeting D2S441 and D3S1358 (online resource 4) (Urquhart et al 1995;Krenke et al 2002) were also run under the above conditions.…”

Section: Y-chromosomal and Autosomal Str Analysesmentioning

confidence: 99%

DNA profiling of Hungarian King Béla III and other skeletal remains originating from the Royal Basilica of Székesfehérvár

Olasz

Seidenberg²,

Hummel³

et al. 2018

Archaeol Anthropol Sci

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A few decades after the collapse of the Avar Khaganate (c. 822 AD), Hungarian invaders conquered the Carpathian Basin (c. 862-895 AD). The first Hungarian ruling dynasty, the Árpáds played an important role in European history during the Middle Ages. King Béla III (1172-1196) was one of the most significant rulers of the dynasty. He also consolidated Hungarian dominance over the Northern Balkans. The provostry church of the Virgin Mary (commonly known as the Royal Basilica of Székesfehérvár) played a prominent role as a coronation church and burial place of medieval Hungarian kings. The basilica's building and graves had been destroyed over the centuries. The only royal graves that remained intact were those of King Béla III and his first spouse, Anna of Antioch. These graves were discovered in 1848. We defined the autosomal STR (short tandem repeat) fingerprints of the royal couple and eight additional individuals (two females and six males) found in the Royal Basilica. These results revealed no evidence of first-degree relationship between any of the investigated individuals. Y-chromosomal STR profiles were also established for all the male skeletons. Based upon the Y-chromosomal data, one male skeleton showed an obvious patrilineal relationship to King Béla III. A database search uncovered an existing Y-chromosomal haplotype, which had a single-repeat difference compared to that of King Béla. It was discovered in a person living in an area close to Hungary. This current male line is probably related paternally to the Árpád Dynasty. The control region of the mitochondrial DNA was determined in the royal couple and in the remains of the inferred relative. The mitochondrial results excluded sibling relationship between the King and the patrilineal relative. In summary, we successfully defined a Y-chromosomal profile of King Béla III, which can serve as a reference for the identification of further remains and disputed living descendants of the Árpád Dynasty. Among the examined skeletons, we discovered an Árpád member, whose exact affiliation, however, has not yet been established.

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Section: Y-chromosomal and Autosomal Str Analysesmentioning

confidence: 99%

DNA profiling of Hungarian King Béla III and other skeletal remains originating from the Royal Basilica of Székesfehérvár

Olasz

Seidenberg²,

Hummel³

et al. 2018

Archaeol Anthropol Sci

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show abstract

“…In the field of forensic DNA analysis including identification, stain analysis and kinship testing, the investigation of short tandem repeat (STR) markers of nuclear DNA has proven to be a valuable and sensitive method [13,15,18].…”

Section: Introductionmentioning

confidence: 99%

Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip

et al. 2006

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Low volume (LV) amplification (1 μL) of nuclear DNA (nucDNA) on a chemically structured chip is an appropriate and highly sensitive method to simultaneously amplify amelogenin and 15 forensically relevant short tandem repeats (STR). In this study, a combined method using on-chip LV amplification of mitochondrial DNA (mtDNA) and subsequent on-chip LV cycle sequencing was established to obtain a method, which is sensitive and robust enough to allow reliable analysis of DNA amounts representing the single cell level. All the necessary steps of the procedure-except for the purification of the sequencing products-were accomplished within the same final 2-μL reaction volume.

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“…None of the other species investigated exhibited amplification signal except for chimpanzee, suggesting that the developed triplex assay is specific for human DNA and some higher primates. Cross reactivity of the human DNA quantification assays as well as STR profiling systems to higher primates is reported by many investigators [6][7][8][9][10][11].…”

Section: Assay Description and Developmentmentioning

confidence: 99%

Quantifiler® Duo DNA Quantification Kit: A Guiding Tool for Short Tandem Repeat Genotyping of Forensic Samples

Barbisin¹,

Fang²,

Furtado³

et al. 2011

J Forensic Res

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Validation of a 16-Locus Fluorescent Multiplex System

Cited by 263 publications

References 9 publications

DNA profiling of Hungarian King Béla III and other skeletal remains originating from the Royal Basilica of Székesfehérvár

DNA profiling of Hungarian King Béla III and other skeletal remains originating from the Royal Basilica of Székesfehérvár

Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip

Quantifiler® Duo DNA Quantification Kit: A Guiding Tool for Short Tandem Repeat Genotyping of Forensic Samples

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