Validation of a LC method for the determination of 5-aminosalicylic acid and its metabolite in plasma and urine

Bystrowska, Beata; Nowak, Jolanta; Brandys, J

doi:10.1016/s0731-7085(99)00292-7

Cited by 47 publications

(34 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Methodsmentioning

confidence: 99%

“…5-AcASA was synthesized by the reaction of 5-ASA with acetic anhydride under basic conditions, as described by other researchers. [11][12][13][14] N-Acetyl-Conjugation by Cultured Hepatocytes Hepatocytes were isolated by the collagenase perfusion method from male Wistar rats weighing 180-220 g provided with standard rat chow and water ad libitum.15) The isolated cells were diluted to make a concentration of 0.5ϫ10 6 cells/ml, in Williams medium containing dexamethasone (1 mM), insulin (0.1 mM), tri-iodothyronine (1 mg/ml), d-aminolevulinic acid hydrochloride (0.2 mM) and 10% fetal calf serum. The suspended cells were seeded on 35 mm plastic culture dishes at a density of 1ϫ10 6 cells/dish, then placed in an incubator in an atmosphere of 5% CO 2 95% air at 37°C.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Inhibitory Effect of Flavonoids on N-Acetylation of 5-Aminosalicylic Acid in Cultured Rat Hepatocytes

Mizoyama

Takaki

Sugihara

et al. 2004

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Flavonoids, which have hydroxyl substitutions in their structure of 2-phenyl-benzopyrone-4-one, are plant-derived components with low toxicity.1,2) Because flavonoids are excellent substrates for sulfatase and glucuronidase, flavonoids administered are predominantly metabolized by sulfo-and glucurono-conjugative routes in intestine and liver cells prior to excretion in vertebrates. [3][4][5] We have recently found that flavonoids have inhibitory effects on the phase II metabolic pathway of acetaminophen in cultured rat hepatocytes.6) This study has expanded our previous findings on the inhibitory effect of flavonoids on N-acetyl-conjugation of 5-aminosalicylic acid (5-ASA), which represents the therapeutically active moiety of the sulfasalazine molecule in the treatment of inflammatory bowel disease. [7][8][9][10] We report in this study for the first time that certain flavonoids also possess potency in inhibiting the formation of N-acetyl-5-ASA (5-AcASA) from 5-ASA, another phase II conjugation reaction, in rat cultured hepatocytes. The potency of such flavonoids in inhibiting the N-acetylation of 5-ASA in the cell-free enzymatic preparation was very similar to that in cultured hepatocytes. Our observations suggest the potential clinical application of flavonoids as the promoting agents in 5-ASA therapy for patients with inflammatory bowel diseases. MATERIALS AND METHODS MaterialsMaterials and chemical reagents were purchased from the following companies: flavonoids from Funakoshi Co. (Tokyo, Japan); 5-ASA, acetyl coenzyme A and other chemicals used for cell culture from Wako Pure Chemical Co. (Osaka, Japan); and the Develosil RPAQUEOUS C-30-UG-3 column (4.6 I.D.ϫ150 mm) from Nomura Chemical Co. (Aichi, Japan). 5-AcASA was synthesized by the reaction of 5-ASA with acetic anhydride under basic conditions, as described by other researchers. [11][12][13][14] N-Acetyl-Conjugation by Cultured Hepatocytes Hepatocytes were isolated by the collagenase perfusion method from male Wistar rats weighing 180-220 g provided with standard rat chow and water ad libitum.15) The isolated cells were diluted to make a concentration of 0.5ϫ10 6 cells/ml, in Williams medium containing dexamethasone (1 mM), insulin (0.1 mM), tri-iodothyronine (1 mg/ml), d-aminolevulinic acid hydrochloride (0.2 mM) and 10% fetal calf serum. The suspended cells were seeded on 35 mm plastic culture dishes at a density of 1ϫ10 6 cells/dish, then placed in an incubator in an atmosphere of 5% CO 2 95% air at 37°C. The monolayers of hepatocytes were cultured for 24 h in Eagle's MEM containing dexamethasone, insulin, tri-iodothyronine, daminolevulinic acid hydrochloride and 10% calf serum. Flavonoids and 5-ASA were dissolved in dimethyl sulfoxide and added to the cultures at definite concentrations, with the final concentration of dimethyl sulfoxide being about 1%. 5-ASA in a stock solution at 50 mM was added to the cultures at a final concentration of 200 mM at 10 min after the addition of flavonoids. After being incubated for 2 h at 37°C, 0.2 ml of the medi...

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Inhibitory Effect of Flavonoids on N-Acetylation of 5-Aminosalicylic Acid in Cultured Rat Hepatocytes

Mizoyama

Takaki

Sugihara

et al. 2004

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…1 A number of analytical methods has been developed for the analysis of mesalamine in pharmaceutical dosage forms and biological matrices. These methods include voltammetry, 2-4 spectrophotometry, 5,6 spectrofluorometry, 7,8 coulometery, 9 and high-performance liquid chromatography (HPLC) combined with UV, [10][11][12] fluorescence, 13,14 mass spectrometry (MS), 15, 16 and electrochemical (EC) 17 detections. Mesalamine is usually present at trace levels in a complex biological matrix, and the potentially interfering endogenous substances (which are usually present at higher concentrations than the drug) need to be removed before the analysis.…”

Section: Introductionmentioning

confidence: 99%

Determination of mesalamine by spectrofluorometry in human serum after solid-phase extraction with Ni-Al layered double hydroxide as a nanosorbent

Abdolmohammad‐Zadeh¹,

Kohansal²

2012

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Hidróxido duplo lamelar de níquel-alumínio nanoestruturado foi sintetizado e o potencial do material obtido, foi avaliado como sorvente para extração em fase sólida (SPE), na separação e pré-concentração de quantidades traço de mesalazina (ácido 5-aminossalicílico) usando o método de coluna. O analito retido em Ni-Al LDH foi removido por solução de NaOH e a concentração de 5-ASA eluída foi então determinada espectrometricamente em λ em = 480 nm com excitação em λ ex = 340 nm. Vários parâmetros experimentais afetando a eficiência da extração de mesalazina em Ni-Al (NO 3 -) LDH, tais como pH, quantidade de sorvente, taxa de fluxo da quantidade de amostra, condições de eluição e volume de amostra, foram investigados. Nas condições experimentais ideais, o limite de detecção e o fator de enriquecimento foram 0,04 e 40 µg L −1 , respectivamente. A curva de calibração do sistema de pré-concentração foi linear no intervalo de 0,1-45,0 µg L −1 com um coeficiente de correlação 0,998. O desvio padrão relativo resultante da análise de seis repetições de soluções de 100 mL contendo 1,0 µg L -1 de mesalazina foi 2,05%. O método otimizado foi aplicado com sucesso na determinação de mesalazina em amostras de soro de sangue. Nanostructured nickel-aluminum layered double hydroxide (Ni-Al LDH) was synthesized and the potential of the obtained material, as solid-phase extraction (SPE) sorbent, for separation and pre-concentration of trace amount of mesalamine (5-aminosalicylic acid) was assessed using column method. The retained analyte on Ni-Al LDH was eluted with NaOH solution and the concentration of the elueted 5-ASA was then spectrofluorometrically determined at λ em = 480 nm with excitation at λ ex = 340 nm. Various experimental parameters affecting the extraction efficiency of mesalamine on Ni-Al (NO 3 -) LDH, such as pH, amount of sorbent, sample loading flow rate, elution conditions and sample volume, were investigated. In the optimum experimental conditions, the limit of detection and enrichment factor were 0.04 and 40 µg L −1 , respectively. The calibration graph for the pre-concentration system was linear in the range of 0.1-45.0 µg L −1 with a correlation coefficient of 0.998. The relative standard deviation (RSD) resulting from the analysis of six replicates of 100 mL solutions containing 1.0 µg L -1 mesalamine was 2.05%. The optimized method was successfully applied to the determination of mesalamine in blood serum samples.

show abstract

“…8 Various other techniques such as UVspectrophotometry, 9 potentiometric titration, 10 micellar electrokinetic chromatography, 11 differential pulse voltammetry, 12 HPLC and LC/ MS/MS have been reported for assaying of MSL in pharmaceutical dosage forms and biological fluids. [13][14][15][16][17][18][19][20][21][22][23][24][25][26] Visible spectrophotometry, because of simplicity and cost effectiveness, sensitivity and selectivity and fair accuracy and precision, has remained competitive in the era of chromatographic techniques for pharmaceutical analysis. Few visible spectrophotometric methods are found in the literature for the assay of MSL.…”

Section: Introductionmentioning

confidence: 99%

Simple and sensitive spectrophotometric methods for the analysis of mesalamine in bulk and tablet dosage forms

Chandra¹,

Bhogela²,

Shaik³

et al. 2011

Quím. Nova

View full text Add to dashboard Cite

Recebido em 26/10/10; aceito em 30/1/11; publicado na web em 1/4/11 Three simple, sensitive, economical and reproducible spectrophotometric methods (A, B and C) are described for determination of mesalamine in pure drug as well as in tablet dosage forms. Method A is based on the reduction of tungstate and/or molybdate in Folin Ciocalteu's reagent; method B describes the reaction between the diazotized drug and α-naphthol and method C is based on the reaction of the drug with vanillin, in acidic medium. Under optimum conditions, mesalamine could be quantified in the concentration ranges, 1-30, 1-15 and 2-30 µg mL -1 by method A, B and C, respectively. All the methods have been applied to the determination of mesalamine in tablet dosage forms. Results of analysis are validated statistically.

show abstract

Validation of a LC method for the determination of 5-aminosalicylic acid and its metabolite in plasma and urine

Cited by 47 publications

References 5 publications

Inhibitory Effect of Flavonoids on N-Acetylation of 5-Aminosalicylic Acid in Cultured Rat Hepatocytes

Inhibitory Effect of Flavonoids on N-Acetylation of 5-Aminosalicylic Acid in Cultured Rat Hepatocytes

Determination of mesalamine by spectrofluorometry in human serum after solid-phase extraction with Ni-Al layered double hydroxide as a nanosorbent

Simple and sensitive spectrophotometric methods for the analysis of mesalamine in bulk and tablet dosage forms

Contact Info

Product

Resources

About