Validation of a MLC method with fluorescence detection for the determination of quinolones in urine samples by direct injection

Rambla-Alegre, Maria; Esteve‐Romero, Josep; Carda‐Broch, Samuel

doi:10.1016/j.jchromb.2009.10.007

Cited by 15 publications

(13 citation statements)

References 24 publications

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“…Thus, to avoid these problems new strategies have been described in the literature. Micellar liquid chromatography (MLC) was developed by RamblaAlegre et al [89] for the determination of five fluoroquinolones in urine samples. This technique is an RP-HPLC procedure with a micellar mobile phase that enables the direct injection of diluted urine samples into the chromatographic system without any further pre-treatment steps.…”

Section: Chromatographic Separationmentioning

confidence: 99%

“…The advantages of direct injection HPLC methods are evident and include simple sample preparation, shorter analysis time, low cost of analysis and good recovery of analytes [99,143]. However, MLC offers poor resolution; this is the reason why Rambla-Alegre et al [89] had to use two different sets of analytical conditions to analyse the fluoroquinolones of two different groups because the simultaneous separation of all five fluoroquinolones was not possible. The column-switching technique requires special and additional equipment for the analysis so is not feasible in all laboratories.…”

Section: Chromatographic Separationmentioning

confidence: 99%

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Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

et al. 2012

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Fluoroquinolones are one of the most promising and intensively studied drugs of contemporary anti-infective chemotherapy. New fluoroquinolone antibacterials with improved pharmacokinetic properties and a broad spectrum of activity have been developed, opening new windows of opportunity for clinical use. To our knowledge, no comprehensive and critical review of the analytical methods for the determination of these agents, which correspond to the third- and fourth-generation quinolones, has yet been published. This work summarizes for the first time most of the liquid chromatographic methods reported in the literature for the separation and quantification of the new fluoroquinolones in biological matrices and pharmaceutical formulations. A systematic and detailed survey of physicochemical properties, sample preparation procedures, and chromatographic and detection conditions is presented herein. In the course of this review several liquid chromatographic methods are discussed: reversed-phase high-performance liquid chromatography (RP-HPLC), ion-exchange high-performance liquid chromatography (IEX-HPLC), hydrophilic interaction liquid chromatography (HILIC), high-performance thin-layer chromatography (HPTLC) and other chiral chromatographic methods. Their advantages, applicability and limitations are also examined.

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Section: Chromatographic Separationmentioning

confidence: 99%

Section: Chromatographic Separationmentioning

confidence: 99%

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

et al. 2012

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“…These results are in good conformity with the data found in the literature. 12 Figure 6B shows urinary elimination curve of OFL following 200 mg oral dose.…”

Section: Pharmacokinetic Study Of Oflmentioning

confidence: 99%

Analysis of ofloxacin and flavoxate HCl either individually or in combination via a green chromatographic approach with a pharmacokinetic study of ofloxacin in biological samples

2015

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New sensitive and rapid micellar liquid chromatographic method was developed for determination of ofloxacin (OFL) and flavoxate hydrochloride (FLV) in different pharmaceutical formulations in addition to a pharmacokinetic study of OFL in human plasma and urine. The analyses were carried out on BDS Hypersil phenyl column (4.6 × 250 mm, 5 µm particle size) using a micellar mobile phase consisting of 0.15 M SDS, 15% n-propanol, 0.3% triethylamine and 0.02 M orthophosphoric acid (pH 2.5) with UV detection at 325 nm. The proposed method was found to be rectilinear over the concentration range of 2.0-40.0 µg mL -1 for the two drugs with lower detection limits of 0.16 and 0.32µg mL -1 for OFL and FLV, respectively. The proposed method was applied for determination of OFL and FLV in their single ingredient and co-formulated dosage forms with good percentage recoveries (99.86-99.98 %) and small standard deviation values (± 0.18 -1.26). The results of the proposed method were statistically compared with those obtained by the comparison methods revealing no significant differences in the performance of the two methods regarding accuracy and precision. To achieve high sensitivity that allows pharmacokinetic study of OFL, fluorescence detection at 290/485 nm was employed. A linear relationship was achieved over the concentration ranges of 0.01-0.10 and 0.02-0.20 µg mL -1 with mean percentage recoveries of 98.63 ± 9.84 and 100.63 ± 3.15 % for OFL in human plasma and urine, respectively.

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“…However, one of the most significant and unique advantages of micellar chromatography is the possibility of direct injection of body fluids to the chromatographic system (Arunyanart and Cline‐Love, ; Perez‐Martinez et al ., ; Rambla‐Alegre et al ., ; Garcia‐Alvarez‐Coque and Carda‐Broch, ; Martin‐Biosca et al ., ; Capella‐Peiro et al ., 2002a, b; Reynolds and Albazi, ; Monferrer‐Pons et al ., ; Chen and Wang, ; Dorsey, ; Cline‐Love et al ., ). Owing to the capability of solubilization of various compounds by the micelles present in the mobile phase, matrices of biological samples are washed out with the front of the eluent, which allows a shorter time of analysis of biological‐origin samples.…”

Section: Introductionmentioning

confidence: 99%

A concise review of applications of micellar liquid chromatography to study biologically active compounds

Stępnik¹

2016

Biomedical Chromatography

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The features of micellar systems are outstanding compared with conventional RP-LC ones. Therefore, the unique properties of micellar chromatography (MLC) are widely recognized. In this short review the applicability of MLC as an in vitro method for the determination of biological activity is discussed. For this purpose many specific examples of MLC applications supported by the theoretical backgrounds of the cited biological activity areas as well as the factors affecting them are presented. This study collects and organizes the most important references of bioactivity determination which were created both recently and in the past, using the MLC method. Although there are many papers on the MLC there is no literature review focused particularly on its applicability in the study of biological activity of various compounds. This work can be treated as a significant review of so far published papers which particularly emphasizes the importance of MLC as in vitro method for determination of bioactivity of different compounds. Copyright © 2016 John Wiley & Sons, Ltd.

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Validation of a MLC method with fluorescence detection for the determination of quinolones in urine samples by direct injection

Cited by 15 publications

References 24 publications

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

Analysis of ofloxacin and flavoxate HCl either individually or in combination via a green chromatographic approach with a pharmacokinetic study of ofloxacin in biological samples

A concise review of applications of micellar liquid chromatography to study biologically active compounds

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