Validation of a multi-residue liquid chromatography–tandem mass spectrometry confirmatory method for 10 anticoccidials in eggs according to Commission Decision 2002/657/EC

Multiclass screening method based on solvent extraction and liquid chromatography–tandem mass spectrometry for the determination of antimicrobials and mycotoxins in egg

“…The procedures commonly employed for antimicrobial multiclass extraction from eggs are pressurized liquid extraction (PLE) [26], and liquid liquid extraction (LLE), either alone [19,21,23] (performed mainly with ACN to induce protein precipitation) or followed by solid phase extraction (SPE) clean-up [17,18,20,24], sometimes with a n-hexane defatting step between LLE and SPE [16,25]. A very interesting study by Frenich et al [22] compared the performances of four extraction techniques, i.e.…”

“…However, in recent years, there is a growing tendency to develop antibiotic multiclass LC-MS methods [12,[16][17][18][19][20][21][22][23][24][25][26]. These studies report the simultaneous determination of the following antimicrobials, respectively: ionophores and macrolides (MCs) [16]; TCs and FQs [18]; SAs, TCs, FQs, ␤-lactams (penicillins and cephalosporins) [17]; coccidiostats and ionophores [19,21,25]; benzimidazoles, MCs, penicillins, FQs, SAs, TCs, nitroimidazoles, coccidiostat, ionophores and amphenicols [20]; TCs, MCs, FQs, SAs and anthelmintics [22]; ionophores, MCs and lincosamides [23]; 105 drug residues including benzimidazoles, nitromidazoles, FQs, SAs, TCs [24]; 41 compounds belonging to SAs, diaminopyridine derivates, FQs, TCs, MCs, penicillins and lincosamides [26].…”

Determination of Coccidiostats in Milk Products by LC–MS and Its Application to a Fermentation Experiment

“…More comprehensive methods such as the ones for eggs [20][21][22][23], feeding stuffs [24,25], muscle [26], and liver [27] are based on liquid chromatography with mass spectrometric detection. This technique provides both the sensitivity and the selectivity required for reliable analysis of complex matrices at the low levels specified by the ML values.…”

Liquid Chromatography Tandem Mass Spectrometry Analysis of Synthetic Coccidiostats in Eggs

“…To this aim, several extraction solvents, extraction time, solvent volume, pH of extraction, clean-up cartridges and solvent for the elution were investigated for the highest recovery of the analytes from the eggs, and the results were evaluated to study the efficacy of the method. At the end, after the optimization of the mass spectrometric parameters and chromatographic conditions, the proposed method was validated according to Commission Decision 2002/657/CE [33,34].…”

“…Starting from the literature about the topic and according to other authors [33], considering the ion suppression in the electrosray ionization to be one of the main problems (when analyzing drugs in complex matrices like eggs), we optimized a liquid-liquid extraction and solid phase extraction (SPE) purification of analytes before the analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in multiple reaction monitoring MRM mode. To this aim, several extraction solvents, extraction time, solvent volume, pH of extraction, clean-up cartridges and solvent for the elution were investigated for the highest recovery of the analytes from the eggs, and the results were evaluated to study the efficacy of the method.…”