Validation of a novel high-sensitivity radioimmunoassay procedure for measurement of total thyroxine concentration in psittacine birds and snakes

Greenacre, Cheryl B.; Young, Diane W.; Behrend, Ellen N.; Wilson, G. Heather

doi:10.2460/ajvr.2001.62.1750

Cited by 27 publications

(12 citation statements)

References 8 publications

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“…Reactive nitrogen species (RNS) are formed in a wide array of diseases, including Alzheimer’s disease, atherosclerosis, and stroke [1]. In these settings, there is robust production of both nitric oxide (NO) and superoxide (O 2 − ), which combine in a near diffusion-rate limited reaction to form peroxynitrite (ONOO − ) [2].…”

Section: Introductionmentioning

confidence: 99%

Protein nitrotryptophan: Formation, significance and identification

Nuriel

Hansler

Gross

2011

Journal of Proteomics

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Section: Introductionmentioning

confidence: 99%

Protein nitrotryptophan: Formation, significance and identification

Nuriel

Hansler

Gross

2011

Journal of Proteomics

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“…3). Generalizações sobre as diferenças nos níveis de hormônios tireoidianos entre espécies não são possíveis, visto que vários fatores influenciam o funcionamento da tireoide, como a disponibilidade de alimentos e de iodo na dieta, a composição de alimentos, a sazonalidade, a temperatura, a idade, a maturidade sexual, a data da coleta de sangue e o estresse (Greenacre et al, 2001;Schmidt e Reavill, 2008). Portanto, os valores do intervalo de referência devem ser utilizados com cuidado (Orosz et al, 2016), devendo-se comparar somente valores gerados pelo mesmo método de análise e, se possível, do mesmo laboratório e entre aves da mesma espécie.…”

Section: Resultsunclassified

Avaliação dos níveis séricos de hormônios tireoidianos em araras (Ara spp.) pelo método de quimioluminescência

Vieira

Cubas

Moraes

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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RESUMO Este trabalho investigou a eficiência do método de quimioluminescência para obtenção das concentrações séricas de hormônios tireoidianos em 23 exemplares de araras [Ara ararauna (n=12) e Ara chloropterus (n=11)], de ambos os sexos [machos (n=11) e fêmeas (n=12)], mantidas em cativeiro. Os valores de T4 total, T4 livre, T3 livre e TSH variaram, respectivamente, de 0,3 a 2,5µg/dL (média=0,94µg/dL), 0,11 a 0,46ɳg/dL (média=0,28ɳg/dL), 1,46 a 3,59pg/mL (média= 2,42pg/mL) e 0,01 a 0,12µUI/mL (média=0,04µUI/mL). Houve interação entre espécie e sexo (P<0,05) sobre as concentrações de T4 total: os machos possuem maiores valores do que as fêmeas de Ara ararauna, enquanto as fêmeas de Ara chloropterus dispõem de níveis mais altos do que os machos desta espécie. Efeito de sexo não foi observado (P>0,05) para os valores de T4 livre, T3 livre e TSH, mas o efeito de espécie foi constatado (P<0,05) para as concentrações de T3 livre. Neste caso, as concentrações de T3 livre são menores em Ara ararauna do que em Ara chloropterus. Os intervalos de referência obtidos sugerem que é possível mensurar as concentrações séricas de hormônios tireoidianos em araras pelo método de quimioluminescência, devendo-se considerar variações relevantes entre diferentes espécies e sexos.

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“…There is considerable relation between thyroid gland function and seasonal variation and energy investment during reproduction and energy store mobilization in breeding snakes [3]. Beyond seasonal and thyroid gland influences, body weight in snakes is also influenced by reproductive aspects of the individual, because high energy demand is associated with reproductive activity of animal [5,21]. Many snakes use a capital breeding strategy rather than one of income breeding [35].…”

Section: Discussionmentioning

confidence: 99%

“…In reptiles thyroid activity appears to influence processes such as shedding [1], growth, development [2] reproduction [3], metabolic rate [4], nutrient assimilation and activity. Many factors also influence thyroid values including age, sex, diurnal changes, seasonal changes, day length, shedding, illness, stress and breeding [5]. In general reptiles have plasma concentrations of thyroid hormones that are far less than mammals [6].…”

Section: Introductionmentioning

confidence: 99%

Serum Level of Thyroid Stimulating Hormone, Triiodothyronine, Thyroxin and Weight Rate in Caucasian Pit Viper (Viperidae: Gloydius Halys Caucasicus) of Iran during Different Seasons

Fani

Vaezi

Todehdehghan

2017

ARRB

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In this research, concentration of thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxin (T4) and their relationship with body weight in Caucasian viper have been studied. The female Caucasian pitviper (n=26) were collected from Lar in Tehran province, the animal's body weight and length, and serum TSH, T3 and T4 levels were measured by electrochemiluminescence method during autumn, winter and summer in year 2014. The results showed T3 hormone concentration in autumn was 0.74± 0.06 nmol/Lt that was higher than its level in winter, 0.46± 0.8 Fani et al.; ARRB, 14(1): 1-9, 2017; Article no.ARRB.33828 2 nmol/Lt (P≤0.05) and no significant different with its concentration in summer 0.76 ± 0.05 nmol/Lt. The concentration of T4 hormone in autumn, 0.84±0.28 nmol/Lt was higher than winter 0.76±0.033 nmol/Lt (P≤0.19) and also summer 0.64 ±0.018 nmol/Lt (P≤0.001). TSH concentration was 0.005 nmol/Lt in autumn; reach to 0.013±0.008 nmol/Lt in winter and again 0.005 nmol/Lt in summer that shows relative increase in winter (P≤0.05). Mean body length and weight of female Caucasian vipers were 47.50±0.69 cm and 50.80±2.53 gram in autumn and 34.33±2.33 cm, 43.33±2.40 gram in winter and 51.64±1.26 cm and 64.78±3.47 gram in summer, respectively. This is indicated that Caucasian vipers' body weight is decreased in winter could be because of body fat consumption, inaccessibility to food and beginning of reduction of nutritional activity at the end of autumn compare to summer that may result to lower body weight in winter. Concentration of T3 and TSH hormone is decreased in summer and increased in winter. It seems by decreasing temperature in winter serum concentration of TSH is increased and viper`s body weight reduced (Fat consumption) to provide required energy and heat for Iranian Caucasian viper survival. So, seasonal and temperature variation has significant effect on Iran Caucasian viper`s thyroid hormones that is important for animal maintenance. Although further study is needed. Original Research Article

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Validation of a novel high-sensitivity radioimmunoassay procedure for measurement of total thyroxine concentration in psittacine birds and snakes

Abstract: This new RIA method provides a commercially available, accurate, and sensitive method for measurement of the relatively low serum T4 concentrations of birds and snakes. Initial ranges for the species evaluated were established.

Cited by 27 publications

References 8 publications

Protein nitrotryptophan: Formation, significance and identification

Protein nitrotryptophan: Formation, significance and identification

Avaliação dos níveis séricos de hormônios tireoidianos em araras (Ara spp.) pelo método de quimioluminescência

Serum Level of Thyroid Stimulating Hormone, Triiodothyronine, Thyroxin and Weight Rate in Caucasian Pit Viper (Viperidae: Gloydius Halys Caucasicus) of Iran during Different Seasons

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