Validation of a Real-Time Quantitative Polymerase Chain Reaction Method for the Quantification of 3 Survivin Transcripts and Evaluation in Breast Cancer Tissues

Pavlidou, Anastasia; Kroupis, Christos; Goutas, Nikolaοs; Dimas, Kostas

doi:10.1016/j.clbc.2013.10.012

Cited by 9 publications

(14 citation statements)

References 40 publications

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“…These findings suggest that the regulation of apoptosis is different in these two histological types [10] . In a previous study of ours (Pavlidou et al [4] ) we have reported that survivin-2b, survivin-ΔEx3 and the ratio of survivin-2b/wild-type survivin showed in vivo a positive correlation with the grade of the tumor in breast cancer samples (P < 0.05). The two isoforms presented an increased expression in advanced histopathologic grade, a important for designing future strategies of therapy by using specific survivin transcripts as targets.…”

Section: Survivin-3bmentioning

confidence: 52%

“…Wt and surΔΕx3 were correlated posively with apoptosis Pavlidou et al [4] wt, sur2b, surΔΕx3 Real time qPCR Tumor grade, estrogen receptors Sur2b/wt had significant association with estrogen receptors Athanassiadou et al [24] wt Immunocytochemistry, immunohistochemistry finding that was expected for survivin-ΔEx3 but not for survivin-2b due to its pro-apoptotic activity. The fact that the two isoforms are elevated in high grade tumors makes them possible markers of tumor aggressiveness.…”

Section: Examined Variants Methods Association With Patient Charactermentioning

confidence: 99%

“…There are numerous factors associated with the occurrence of breast cancer, such as: genetic susceptibility arising from mutations in high/moderate penetrance genes (such as BRCA1/2, PALB2, CHEK2, BRIP1, RAD50, NSB1 etc.) [2][3] , hormone-associated reproductive factors such as increased menstrual cycles arising from either earlier age at menarche or later age at menopause or decreased parity, older age at first birth and use of hormone therapy; consumption of alcohol and type of diet; obesity; exposure to radiation; atypical hyperplasia of the mammary gland [4] . Inhibition of apoptosis causes tumorigenesis through cell survival resulting in accumulation of genetic mutations that lead normal tissues to transformation.…”

Section: Topic Highlightmentioning

confidence: 99%

“…b: Sequence originated from intron; 3'UTR: Untranslated region, the red lines represent the baculovirus inhibitors of apoptosis repeat domain (modified from ref. [4] and [20]). …”

Section: Survivin-3bmentioning

confidence: 99%

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Association of survivin splice variants with prognosis and treatment of breast cancer

Pavlidou

Kroupis

Dimas

2014

WJCO

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The purpose of this study was the overview of current knowledge regarding the use of survivin and its isoforms in prognosis and treatment of breast cancer. An advanced search of Medline was performed using the following search strategy: "(survivin isoforms) OR (survivin transcript variants) AND (breast cancer) AND (neoplasm OR tumor OR cancer OR carcinoma)". Relevant studies were retrieved and processed thoroughly in order to analyze the related data. Besides wild-type survivin full-length transcript, another six splice variants have been identified. Overexpression of survivin and its isoforms leads to shorter overall and disease-free survival; the transcript variants are correlated with apoptosis and could assist prognosis prediction. It has been proved through numerous studies that inhibiting survivin isoforms might become a promising target of drug therapy of carcinomas. Use of small molecule YM155 could offer new therapy for triple negative breast cancer patients, while, chemotherapy with 5-fluorouracil + epirubicin + cyclophosphamide and Tax-Epi could be guided by survivin splice variants measurements. Survivin transcript variants could become prognostic biomarkers and could provide information about clinical management of patients suffering from breast cancer.© 2014 Baishideng Publishing Group Inc. All rights reserved.Key words: Survivin gene; Isoforms; Therapy; Prognosis; Breast cancer Core tip: Besides wild type survivin full length transcript, another six splice variants have been identified. Overexpression of survivin and its isoforms leads to shorter overall and disease-free survival; the transcript variants are positively correlated with apoptosis and could assist prognosis prediction. It has been proved through numerous studies that inhibiting survivin isoforms might become a promising target of drug therapy of carcinomas. Use of small molecule YM155 could offer new therapy for triple negative breast cancer patients while, chemotherapy with 5-fluorouracil + epirubicin + cyclophosphamide and Tax-Epi could be guided by survivin splice variants measurements. Survivin transcript variants could become prognostic biomarkers and could provide information about clinical management of patients suffering from breast cancer.Pavlidou A, Kroupis C, Dimas K. Association of survivin splice variants with prognosis and treatment of breast cancer. World J

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Section: Survivin-3bmentioning

confidence: 52%

Section: Examined Variants Methods Association With Patient Charactermentioning

confidence: 99%

Section: Topic Highlightmentioning

confidence: 99%

“…b: Sequence originated from intron; 3'UTR: Untranslated region, the red lines represent the baculovirus inhibitors of apoptosis repeat domain (modified from ref. [4] and [20]). …”

Section: Survivin-3bmentioning

confidence: 99%

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Association of survivin splice variants with prognosis and treatment of breast cancer

Pavlidou

Kroupis

Dimas

2014

WJCO

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“…The cDNA samples were then stored at −20 °C. In order to test the quality and purity of RNA samples, the resulting cDNA was amplified in a control PCR method of the actin reference gene as previously described [ 33 ]. cDNA samples that are free of containing genomic DNA produce a unique fragment of 587 base pairs (bp) (and not the additional fragment of 1122 bp if genomic DNA exists).…”

Section: Methodsmentioning

confidence: 99%

Development of novel real-time PCR methodology for quantification of COL11A1 mRNA variants and evaluation in breast cancer tissue specimens

et al. 2015

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BackgroundCollagen XI is a key structural component of the extracellular matrix and consists of three alpha chains. One of these chains, the α1 (XI), is encoded by the COL11A1 gene and is transcribed to four different variants at least (A, B, C and E) that differ in the propensity to N-terminal domain proteolysis and potentially in the way the extracellular matrix is arranged. This could affect the ability of tumor cells to invade the remodeled stroma and metastasize. No study in the literature has so far investigated the expression of these four variants in breast cancer nor does a method for their accurate quantitative detection exist.MethodsWe developed a conventional PCR for the general detection of the general COL11A1 transcript and real-time qPCR methodologies with dual hybridization probes in the LightCycler platform for the quantitative determination of the variants. Data from 90 breast cancer tissues with known histopathological features were collected.ResultsThe general COL11A1 transcript was detected in all samples. The developed methodologies for each variant were rapid as well as reproducible, sensitive and specific. Variant A was detected in 30 samples (33 %) and variant E in 62 samples (69 %). Variants B and C were not detected at all. A statistically significant correlation was observed between the presence of variant E and lymph nodes involvement (p = 0.037) and metastasis (p = 0.041).ConclusionsWith the newly developed tools, the possibility of inclusion of COL11A1 variants as prognostic biomarkers in emerging multiparameter technologies examining tissue RNA expression should be further explored.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-015-1725-8) contains supplementary material, which is available to authorized users.

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Development and validation of molecular methodologies to assess PALB2 expression in sporadic breast cancer

Poumpouridou

Acha-Sagredo

Goutas

et al. 2016

Clinical Biochemistry

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Validation of a Real-Time Quantitative Polymerase Chain Reaction Method for the Quantification of 3 Survivin Transcripts and Evaluation in Breast Cancer Tissues

Cited by 9 publications

References 40 publications

Association of survivin splice variants with prognosis and treatment of breast cancer

Association of survivin splice variants with prognosis and treatment of breast cancer

Development of novel real-time PCR methodology for quantification of COL11A1 mRNA variants and evaluation in breast cancer tissue specimens

Development and validation of molecular methodologies to assess PALB2 expression in sporadic breast cancer

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