Validation of an analytical method for the quantitative determination of selenium in bacterial biomass by ultraviolet–visible spectrophotometry

Mörschbächer, Ana Paula; Dullius, Anja; Dullius, Carlos Henrique; Bandt, Cassiano Ricardo; Kuhn, Daniel; Brietzke, Débora Tairini; Kuffel, Fernando José Malmann; Etgeton, Henrique Pretto; Altmayer, Taciélen; Gonçalves, Tamara Engelmann; Oreste, Eliézer Quadro; Ribeiro, Anderson Schwingel; Souza, Cláucia Fernanda Volken de; Hoehne, Lucélia

doi:10.1016/j.foodchem.2018.02.057

Cited by 30 publications

(12 citation statements)

References 29 publications

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“…Microbial growth of each LAB strain in the presence of Se was monitored by determining the optical density (OD) of the culture medium considering the moment of inoculation as time point zero and using sterile MRS broth as the control. Growth curves were obtained by measuring the OD every 2 h in a UV-Vis molecular absorption spectrophotometer at the 600-nm wavelength (OD 600nm ) until the cells reached the stationary phase (Pieniz et al, 2011;Deng et al, 2015;Mörschbächer et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

“…Se Quantification. Selenium concentrations in bacterial biomasses were determined using UV-Vis molecular absorption spectroscopy (Mörschbächer et al, 2018). For the calibration curve, aliquots corresponding to 0.025 to 0.250 mg/L of Se were taken from a stock solution and were transferred to a series of volumetric 10-mL flasks, to which 1 mL of 2% (mass/vol.)…”

Section: Methodsmentioning

confidence: 99%

“…However, most of these methods require sample preparation steps to enhance their analytical sensitivity, specific training, and high-cost equipment and are susceptible to analyte losses due to the number of steps involved (Agrawal et al, 2009;Kanchi et al, 2017). Conversely, UV and visible (UV-Vis) molecular absorption spectroscopy is simple, easy to operate, and selective for Se quantification in complex samples, as reported in the literature (Narayana et al, 2003;Agrawal et al, 2009;Mörschbächer et al, 2018). Thus, developing a method based on this analytical method is important for Se quantification.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of selenium bioaccumulation in lactic acid bacteria

Mörschbächer

Dullius

et al. 2018

Journal of Dairy Science

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Selenium is an essential micronutrient for living beings, as it helps to maintain the normal physiological functions of the organism. The numerous discoveries involving the importance of this element to the health of human beings have fostered interest in research to develop enriched and functional foods. The present study evaluated the potential for bacterial strains of Enterococcus faecalis (CH121 and CH124), Lactobacillus parabuchneri (ML4), Lactobacillus paracasei (ML13, ML33, CH135, and CH139), and Lactobacillus plantarum (CH131) to bioaccumulate Se in their biomass by adding different concentrations of sodium selenite (30 to 200 mg/L) to the culture medium. Quantification of Se with UV and visible molecular absorption spectroscopy showed that the investigated bacteria were able to bioaccumulate this micromineral into their biomass. Two of the L. paracasei strains (ML13 and CH135) bioaccumulated the highest Se concentrations (38.1 ± 1.7 mg/g and 40.7 ± 1.1 mg/g, respectively) after culture in the presence of 150 mg/L of Se. This bioaccumulation potential has applications in the development of dairy products and may be an alternative Se source in the diets of humans and other animals.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of selenium bioaccumulation in lactic acid bacteria

Mörschbächer

Dullius

et al. 2018

Journal of Dairy Science

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“…Moreover these methods have many individual limitations like use of sophisticated instruments, time consuming operation, requires multi-step procedures for pre-concentration of analyte and low selectivity with higher interference problems. In addition to the above-mentioned methods, studies on molecular spectroscopy-based articles show some drawbacks such as having low molecular absorption coefficients in the ultraviolet region, lack of any report about cationic interferences studies, and in some cases the reported detection limits are not competitive with the results of atomic spectroscopic techniques [20][21][22][23][24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Determination of Selenium (IV) Using 4,5-diamino-o-xylene as a New Chromogenic Reagent

Tavancheh

Beiraghi

2020

Adv. J. Chem. A

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A simple, rapid, and sensitive spectrophotometric method for the determination of selenium (IV) based on the formation of a 1:2 complex with 4,5-diamino-o-xylene (DAX) as a new sensitive and selective chromogenic reagent is developed. The DAX reacts with selenium (IV) in the presence of citric acid to form a complex in the pH range of 1.0≃2.2. The experimental conditions for determining selenium including the influences of pH, reagent concentration and time were evaluated and optimized. Under the optimum experimental conditions, the molar absorptivity of the complex was 1.27×10 4 L/mol-1 cm-1 at 340 nm. Beer's law is obeyed in the range of 1≃12 µg/mL-1. The limits of detection and quantization were 0.948 µg mL-1 and 3.16 µg/mL-1 respectively. The interfering effect of some cations and anions was also studied. This method can be applied to the direct and easy determination of selenium species as organic, inorganic, Se(VI) and Se(IV) in a variety of real samples. 4, 5-Diamino-o-xylene (DAX) Spectrophotometry Selenium (IV) Selenium (VI)

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“…The on-line detection process mainly includes sampling system, automatic sequential injection system and detection system, among them, the detection system is a key factor, which requires the detection instrument to have the characteristics of miniaturization, integration, and fast detection speed, so as to be suitable for online detection of multi-component substances in the industrial field. In recent years, there have been some methods for on-line determination of metal ions, such as polarography [17], potentiometric titration [18], laserinduced breakdown spectroscopy [19], ultraviolet-visible absorption spectroscopy [20], and inductively coupled plasma mass spectrometry [21]. Among them, ultraviolet-visible absorption spectroscopy is widely used in the field of analysis and detection for its advantages such as versatility, speed, simplicity, accuracy and low cost [22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Multi-Objective Optimized Overlapping Peak Separation Algorithm for Simultaneous Detection of Copper and Cobalt by Ultraviolet-Visible Spectroscopy

Zhou

Zhu

2020

IEEE Access

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In the zinc hydrometallurgy, the excessive impurity ions of copper and cobalt are very harmful to the electrolysis process, which affects product quality and wastes resources. In order to solve the problems of low sensitivity, severe spectral overlap and narrow effective band, an overlapping peak separation algorithm based on multi-objective optimization was proposed for simultaneous detection of copper and cobalt in high concentration zinc solution by ultraviolet-visible spectroscopy. First, according to the characteristics of the spectral signal, the derivative spectroscopy by continuous wavelet transform was used to separate the spectral overlapping peaks of copper and cobalt and shield the zinc interference. Then, taking decomposition scale as decision variable, and the information occupancy and peak separation as the optimization targets, a multi-objective optimization model was established and solved using the particle swarm multi-objective optimization method. Finally, derivative spectroscopy combined with zero-crossing method was used to establish calibration curves for simultaneous detection of copper and cobalt. The experimental results show that the proposed method is suitable for simultaneous detection of trace amounts of copper and cobalt in high concentration zinc solution.

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Validation of an analytical method for the quantitative determination of selenium in bacterial biomass by ultraviolet–visible spectrophotometry

Cited by 30 publications

References 29 publications

Assessment of selenium bioaccumulation in lactic acid bacteria

Assessment of selenium bioaccumulation in lactic acid bacteria

Spectrophotometric Determination of Selenium (IV) Using 4,5-diamino-o-xylene as a New Chromogenic Reagent

Multi-Objective Optimized Overlapping Peak Separation Algorithm for Simultaneous Detection of Copper and Cobalt by Ultraviolet-Visible Spectroscopy

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