VALIDATION OF AN ANALYTICAL METHOD FOR THE QUANTIFICATION OF GIBERELLIC ACID IN MAIZE SEEDS (Zea mays L.) BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

Gibberellic acid is a phytohormone that triggers the germination of seeds in a state of dormancy. Through the quantification of this hormone, the physiological condition of seeds of economic importance can be studded. In this work we validated a High-Performance Liquid Chromatography method to quantify gibberellic acid in germinated maize (Zea mays L.) seeds. Chromatographic conditions included the use of a C-18 reversed-phase column, acetonitrile-formic acid (1 : 9 %) as the mobile phase, flow of 0.5 mL·min-1, and detection at 195 nm. We evaluated our method for seven analytical parameters. The method was linear for gibberellic acid concentrations from1.0 mg·kg-1 to 50.0 mg·kg-1. The method’s limits were 0.3 mg·kg-1 and1.0 mg·kg-1 for detection and quantification, respectively. The method was highly precise; we obtained variable but low relative standard deviations (2.62 % - 12.66 %) for the studied gibberellic acid concentrations. We assessed accuracy through recovery percentages, ranging from 52.85 % - 63.68 %, for three gibberellic acid concentrations. We conclude that our analytical method can be used to measure gibberellic acid during the early stages of maize germination. In addition, the method could be used for the analysis of other types of plant matrices.

Effective method for simultaneous determination of abscisic acid, 3-indolacetic acid and gibberellic acid in commercial plant biostimulants by capillary electrophoresis with diode array detection

“…Gibberellic acid GA3 is the most common gibberellin (Kwon & Paek, 2016), and it participates during seed germination, stem elongation and the beginning of flowering (Camara et al, 2018). To our knowledge, several studies focus on the individual and simultaneous determination of phytohormones in different matrices have been established using high performance liquid chromatography (HPLC) (Nakurte et al, 2012;Nhujak et al, 2005;Rivera et al, 2020;Spagnuolo et al, 2022;Wu & Hu, 2009), liquid chromatography with tandem mass spectrometry (LC-MS-MS) (Yang et al, 2022), capillary electrophoresis coupled to mass spectrometry (CE-MS) (Tan et al, 2014), as well as capillary electrophoresis coupled to a diode array detector (CE-DAD) (Chen et al, 2018;Huang et al, 2014;Nhujak et al, 2005;Quijano-Avilés et al, 2018;Yang et al, 2016). Furthermore, most of the procedures require sample preparation steps such as solid-liquid extraction, solid-phase extraction (SPE), supercritical fluid extraction (SFE), liquid-phase microextraction (LPME), ultrasound-assisted extraction (UAE), dynamic sonication-assisted solvent extraction (DSASE), among other techniques.…”