Validation of analytical methods for chlordecone and its metabolites in the urine and feces of ewes

Development and Application of Molecularly Imprinted Polymers for the Selective Extraction of Chlordecone from Bovine Serum

“…Capillary and vaporizer temperatures were set at 395 and 277 • C, respectively, and tube lens offsets of 90 and 80 V were used for CLD and CLD-OH, respectively. As in previous studies, CLD was detected under the deprotonated hydrated form and, thus, the transition 506.7 → 426.7 (21 eV) was used to quantify it [11,13]. Regarding CLD-OH, as already reported by He et al [34], the compound was not fragmented (even using high collision energy and the pseudo MRM transition 490.5 → 490.5 was used for quantification purpose).…”

“…Today, GC is still used but coupled with mass spectrometry for its higher specificity, especially at low concentrations [10]. However, more recent studies use liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) preceded, or not, by an isotopic dilution for quantifying CLD in food, tissues, or biological fluids leading to an LOQ of only a few µg kg −1 [11][12][13].…”

“…Then, an additional cleanup of the organic layer is performed with NaOH treatment and acidic lysis with sulfuric acid [10] or with a solid-phase extraction (SPE) step on Florisil ® cartridges [15]. More recently, a method dedicated to the analyses of CLD in animal liver [11,12], feces, and urine of ewes [11,12], based on QuEChERS method, was validated. Concerning the extraction procedure for blood samples (serum [13] or plasma [16]), it is based on a slightly simpler protocol originally dedicated to the extraction of polychlorobiphenyls (PCB) [15].…”

Construction agile d’un programme de recherche-action, interdisciplinaire de longue durée sur polluant organique chlordécone persistant et toxique, pour répondre aux enjeux santé-environnement

“…Il a donc fallu revisiter ces données et concevoir des expérimentations avec des moyens analytiques renouvelés sans bénéficier du radiomarquage. Trois thèses (Lastel, 2015 ;Saint-Hilaire, 2018 ;Fourcot, 2020) se sont succédées pour étudier les processus ADME (Absorption Distribution Métabolisme Excrétion) chez les ruminants d'élevage mobilisant en conditions contrôlées principalement les petits ruminants (chèvre et brebis) avec d'importants développements analytiques (Saint Hilaire et al, 2018α, b). Suite à ces travaux, il apparat que les ruminants sont bien capables d'éliminer la CLD, soit en la transformant en alcool (le chlordécol) soit en l'éliminant directement via la bile.…”