Validation of Clausius–Mossotti Function in Wideband Single-Cell Dielectrophoresis

Du, Xiaotian; Ma, Xiao; Li, Hang; Li, Lei; Cheng, Xuanhong; Hwang, J.C.M.

doi:10.1109/jerm.2019.2894100

Cited by 13 publications

(5 citation statements)

References 18 publications

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“…The crossover frequency fx02 depends on the dielectric parameters of the intracellular content [19]. Hence, cells can be individually electro-manipulated by the DEP force Moreover, it is possible to change the value of the dielectric parameters in order to study the evolution of the real part of the CM factor and the parameter dependency of the two crossover frequencies as has been carried out in [12][13][14]. A first approximation of the crossover frequency f x01 can be expressed as [7]:…”

Section: Effect Of the Cellular Dielectric Properties On The Clausius-mossotti Factormentioning

confidence: 99%

Microfluidic Lab-on-a-Chip Based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

et al. 2021

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Glioblastoma (GBM) is one of the most aggressive solid tumors, particularly due to the presence of cancer stem cells (CSCs). Nowadays, the characterization of this cell type with an efficient, fast and low-cost method remains an issue. Hence, we have developed a microfluidic lab-on-a-chip based on dielectrophoresis (DEP) single cell electro-manipulation to measure the two crossover frequencies: fx01 in the low-frequency range (below 500 kHz) and fx02 in the ultra-high-frequency range (UHF, above 50 MHz). First, in vitro conditions were investigated. An U87-MG cell line was cultured in different conditions in order to induce an undifferentiated phenotype. Then, ex vivo GBM cells from patients’ primary cell culture were passed through the developed microfluidic system and characterized in order to reflect clinical conditions. This article demonstrates that the usual exploitation of low-frequency range DEP does not allow the discrimination of the undifferentiated GBM cells from the differentiated one. However, the presented study highlights the use of UHF-DEP as a relevant discriminant parameter. The proposed microfluidic lab-on-a-chip is able to follow the kinetics of U87-MG phenotype transformation in a CSC enrichment medium and the cancer stem cells phenotype acquirement.

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Section: Effect Of the Cellular Dielectric Properties On The Clausius-mossotti Factormentioning

confidence: 99%

Microfluidic Lab-on-a-Chip Based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

et al. 2021

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“…Moreover, it is possible to change the value of the dielectric parameters in order to study the evolution of the real part of the CM factor and the parameter-dependency of the two crossover frequencies as it has been done in [11][12][13]. A first approximation of the crossover frequency fx01 can be expressed as [6]:…”

Section: Effect Of the Cellular Dielectric Properties On The Clausius-mossotti Factormentioning

confidence: 99%

Microfluidic Lab-On-a-Chip based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

Lambert¹,

Manczak

Barthout³

et al. 2021

Preprint

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Glioblastoma (GBM) is one of the most aggressive solid tumors, particularly due to the presence of cancer stem cells (CSCs). Today the characterization of this type of cells with an efficient, fast and low-cost method remains an issue. Hence, we have developed a microfluidic lab-on-a-chip based on dielectrophoresis (DEP) single cell electro-manipulation to measure the two crossover frequencies: fx01 in low frequency range (below 500 kHz) and fx02 in Ultra High Frequency range (UHF, above 50 MHz). First, in vitro conditions were investigated. U87-MG cell lines were cultured in different conditions in order to induce an undifferentiated phenotype. Then, ex vivo GBM cells from patients’ primary cell culture, were passed through the developed microfluidic system and characterized in order to reflect clinical conditions. This article demonstrates that the usual exploitation of low frequency range DEP does not allow the discrimination of the undifferentiated from the differentiated phenotypes of GBM cells. However, the presented study highlights the use of UHF-DEP as a relevant discriminant parameter. The proposed microfluidic lab-on-a-chip is able to follow the kinetic of U87-MG phenotype transformation in a CSC enrichment medium and their cancer stem cells phenotype acquirement.

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“…The power and frequency are carefully chosen for each function based on previous experiments. For example, the cell is released by not only lowering the frequency to switch from positive DEP to negative DEP, but also by doubling the power to compensate for the lower value of the Clausius‒Mossotti factor at the lower frequency [ 12 ]. The sensing power level is orders-of-magnitude lower than that required for reversible electroporation, let alone heating or otherwise affecting the vitality of the cell [ 17 ].…”

Section: Experimental Preparationmentioning

confidence: 99%

“…The CPW, with such a defected ground, can double as the calibration standard when covered by different liquids. This is because the protrusion is so little that, although it can perturb the local field sufficiently to trap a cell at its tip by dielectrophoresis (DEP) [ 12 ], it does not disturb significantly the overall characteristics of the CPW. Note that the protrusion of 6 μm is smaller than one-thousandth of the wavelength between 0.5 and 5 GHz.…”

Section: Introductionmentioning

confidence: 99%

Broadband Electrical Sensing of a Live Biological Cell with In Situ Single-Connection Calibration

et al. 2020

Sensors

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Single-connection in situ calibration using biocompatible solutions is demonstrated in single-cell sensing from 0.5 to 9 GHz. The sensing is based on quickly trapping and releasing a live cell by dielectrophoresis on a coplanar transmission line with a little protrusion in one of its ground electrodes. The same transmission line is used as the calibration standard when covered by various solutions of known permittivities. The results show that the calibration technique may be precise enough to differentiate cells of different nucleus sizes, despite the measured difference being less than 0.01 dB in the deembedded scattering parameters. With better accuracy and throughput, the calibration technique may allow broadband electrical sensing of live cells in a high-throughput cytometer.

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Validation of Clausius–Mossotti Function in Wideband Single-Cell Dielectrophoresis

Cited by 13 publications

References 18 publications

Microfluidic Lab-on-a-Chip Based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

Microfluidic Lab-on-a-Chip Based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

Microfluidic Lab-On-a-Chip based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

Broadband Electrical Sensing of a Live Biological Cell with In Situ Single-Connection Calibration

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