Validation of HPTLC Method for Estimation of Diosgenin in Callus and Rhizome of Dioscorea deltoidea

Amir, Mohd; Mujeeb, Mohd; Sabih, Abdul-Hamid; Shakeel, Ahmad; Ahmad, Anwar; Siddiqui, WA

doi:10.1055/s-0030-1251797

Cited by 4 publications

(5 citation statements)

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“…The rationale for initiating this chromatographic study was the lack of literature on such work on most of the Dioscorea species mentioned herein [23–25]. The current work also includes determination of the hexanes/ethyl acetate and the methanol extractive values and their respective percentages of the samples of Dioscorea studied based on gravimetric analysis.…”

Section: Introductionmentioning

confidence: 99%

Application of anatomy and HPTLC in characterizing species of Dioscorea (Dioscoreaceae)

et al. 2014

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The edible tubers from different species of Dioscorea are a major source of food and nutrition for millions of people. Some of the species are medicinally important but others are toxic. The genus consists of about 630 species of almost wholly dioecious plants, many of them poorly characterized. The taxonomy of Dioscorea is confusing and identification of the species is generally problematic. There are no adequate anatomical studies available for most of the species. This study is aimed to fill this gap and provides a detailed investigation of the anatomy and micromorphology of the rhizomes and tubers of five different species of Dioscorea, namely D. balcanica, D. bulbifera, D. polystachya, D. rotundata and D. villosa. The primary features that can help in distinguishing the species include the nature of periderm, presence or absence of pericyclic sclereids, lignification in the phloem, types of calcium oxalate crystals and features of starch grains. The descriptions are supported with images of bright-field and scanning electron microscopy for better understanding of these species. The diagnostic key of anatomical features included in this paper can help distinguish the investigated species unambiguously. Additionally, HPTLC analyses of authentic and commercial samples of the five species are described.

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Section: Introductionmentioning

confidence: 99%

Application of anatomy and HPTLC in characterizing species of Dioscorea (Dioscoreaceae)

et al. 2014

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“…This is the first report on enhanced diosgenin production in H. isora cell and hairy root cultures. Owing to its tremendous usage in pharmaceutical industry and market values, diosgenin is one of the most sought after secondary metabolites of plant origin and therefore various methods are being worked out and reported for its enhanced production in plant tissues including plant cell and suspension cultures [ 25 , 29 ], via implying abiotic and biotic stresses or elicitors [ 30 ] and microbial infections [ 1 ]. Narula et al [ 30 ] reported stimulated diosgenin production under CuSO 4 -induced metal stress in Dioscorea bulbifera cultures.…”

Section: Resultsmentioning

confidence: 99%

Hairy Root Induction in Helicteres isora L. and Production of Diosgenin in Hairy Roots

Kumar¹,

Desai²,

Shriram³

2014

Nat. Prod. Bioprospect.

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Mature seeds of Helicteres isora L. were collected from seven geographical locations of Maharashtra and Goa (India) and evaluated for diosgenin (a bioactive steroidal sapogenin of prime importance) extraction and quantification. Chemotypic variations were evidenced with diosgenin quantity ranging from 33 μg g−1 seeds (Osmanabad forests) to 138 μg g−1 (Khopoli region). Nodal and leaf explants from in vitro-raised seedlings were used for callus and Agrobacterium-mediated transformation, respectively. Compact, hard, whitish-green callus (2.65 g explant−1) was obtained on MS + 13.32 μM BAP + 2.32 μM Kin after 30 days of inoculation. Various parameters including types of explant and Agrobacterium strain, culture density, duration of infection and various medium compositions were optimized for hairy root production. A. rhizogenes strain ATCC-15834 successfully induced hairy roots from leaf explants (1 cm2) with 42 % efficiency. Transgenic status of the roots was confirmed by PCR using rolB and VirD specific primers. Hairy roots showed an ability to synthesize diosgenin. Diosgenin yield was increased ~8 times in hairy roots and ~5 times in callus than the seeds of wild plants. Enhanced diosgenin content was associated with proline accumulation in hairy roots. This is the first report on induction of hairy roots in H. isora.

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“…Oleh karena itu, ada kebutuhan mendesak untuk mengembangkan metode sederhana untuk estimasi senyawa ini menggunakan instrumen canggih seperti kromatografi cair kinerja tinggi (HPLC). Beberapa metode analisis telah dikembangkan untuk analisis diosgenin hingga saat ini, antara lain: spektrofotometri HPLC, HPTLC, dan TLC (Chapagain and Wiesman, 2005;Amir et al, 2010, Abdelkarim et al, 2015. Baik metode spektrofotometri maupun metode HPTLC relatif kompleks karena sedikitnya kromofor yang terdapat pada struktur diosgenin, sehingga harus dilakukan derivatisasi.…”

Section: Pendahuluanunclassified

PENETAPAN KADAR DIOSGENIN DALAM EKSTRAK AIR Costus speciosus SECARA HPLC

et al. 2017

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Validation of HPTLC Method for Estimation of Diosgenin in Callus and Rhizome of Dioscorea deltoidea

Cited by 4 publications

References 0 publications

Application of anatomy and HPTLC in characterizing species of Dioscorea (Dioscoreaceae)

Application of anatomy and HPTLC in characterizing species of Dioscorea (Dioscoreaceae)

Hairy Root Induction in Helicteres isora L. and Production of Diosgenin in Hairy Roots

PENETAPAN KADAR DIOSGENIN DALAM EKSTRAK AIR Costus speciosus SECARA HPLC

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