Validation of ISO method 11290 Part 1 — Detection of Listeria monocytogenes in foods

Validation of a PCR-Based Method for Detection of Food-Borne Thermotolerant Campylobacters in a Multicenter Collaborative Trial

“…The PCR results were analyzed statistically according to the recommendations of Scotter et al (23) by the method of Langton et al (14). The interlaboratory diagnostic sensitivity was defined as the percentage of positive samples giving a correct positive signal (8).…”

Pervasiveness of Listeria monocytogenes in Milk and Dairy Products

“…After 1 h standing period 1 ml of the enrichment buffer was pipetted out into a sterile Petri-plate in duplicates and the recovered L. monocytogenes were cultured on, PALCAM-agar (HIMEDIA) medium as per the NF EN ISO 11290-2 procedure [5]. The plates were incubated at 37ºC for 48 h. Grey colored Colonies with black hallo zone were counted and isolated in Tripticase Soy-Agar with Yeast extract (TSAY) (HIMEDIA) and identified through Gram-staining, Cell-motility, Oxidase, Catalase, Indole, Methyl-red, Voges-Proskauer tests, Ureaseproduction, Simon citrate agar, and various sugar-fermentation tests (mannitol, rahmnose, and xylose).…”

An ELISA-on-a-Chip Biosensor System for Early Screening of Listeria monocytogenes in Contaminated Food Products

“…To this end, the fish intestines were collected and employed as real sample matrices. According to the standard protocol for sample preparation, 6 the intestines (10 g) were grinded in 90 mL Listeria enrichment broth (LEB; Merck, Darmstadt, Germany) containing 0.5% yeast extracts.…”

“…Three methods are applicable to microorganism detection: colony culture, which is the conventional method, [6][7][8] genetic approaches that are based on the polymerase chain reaction, [9][10][11] and immunological assays [12][13][14][15] such as enzyme-linked immunosorbent assay (ELISA). Although the conventional method is the most reliable method for detecting the existence of mica These authors equally contributed to this research.…”