Validation of short tandem repeat analysis for the investigation of cases of disputed paternity

Thomson, J. Arthur; Pilotti, V.; Stevens, Peter S.; Ayres, Karen L.; Debenham, Paul G.

doi:10.1016/s0379-0738(98)00199-6

Cited by 60 publications

(45 citation statements)

References 13 publications

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“…In a pilot study, DNA derived from 33 human cancer cell lines was compared by using two STR profile multiplex systems: Second Generation Multiplex (United Kingdom Forensic Science Service) (25) and Powerplex 1 (Promega) (26). The information on cell line identification and assortment obtained was identical in the two systems, with the exception of a single pair of samples that showed identical SGM profiles but an additional This paper was submitted directly (Track II) to the PNAS office.…”

Section: Methodsmentioning

confidence: 99%

Short tandem repeat profiling provides an international reference standard for human cell lines

Masters

Thomson

Daly-Burns

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

436

390

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Cross-contamination between cell lines is a longstanding and frequent cause of scientific misrepresentation. Estimates from national testing services indicate that up to 36% of cell lines are of a different origin or species to that claimed. To test a standard method of cell line authentication, 253 human cell lines from banks and research institutes worldwide were analyzed by short tandem repeat profiling. The short tandem repeat profile is a simple numerical code that is reproducible between laboratories, is inexpensive, and can provide an international reference standard for every cell line. If DNA profiling of cell lines is accepted and demanded internationally, scientific misrepresentation because of cross-contamination can be largely eliminated.

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Section: Methodsmentioning

confidence: 99%

Short tandem repeat profiling provides an international reference standard for human cell lines

Masters

Thomson

Daly-Burns

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

436

390

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“…Microsatellites have the following characteristics: (i) they have a simple and stable inheritance when they are transmitted from one generation to the next; (ii) due to their small size, they are efficiently amplified using PCR techniques and can be easily resolved using polyacrylamide gel or capillary electrophoresis. Accordingly, microsatellites have been used for parentage testing in forensic sciences [8,19,24].…”

mentioning

confidence: 99%

Population Study and Validation of Paternity Testing for Thoroughbred Horses by 15 Microsatellite Loci.

et al. 2001

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ABSTRACT. Microsatellite 15 TKY System was characterized for parentage verification of horse registry. The Microsatellite 15 TKY System was constructed by using 15 microsatellites, TKY279, TKY287, TKY294, TKY297, TKY301, TKY312, TKY321, TKY325, TKY333, TKY337, TKY341, TKY343, TKY344, TKY374, and TKY394, to provide stringent PCR-based microsatellite typing specifically optimized for multicolor fluorescence detection. The Microsatellite 15 TKY System showed good resolutions for 250 unrelated Thoroughbred horses, and the probability of exclusion (PE) at each microsatellite ranged from 0.437 to 0.621, resulting in a total PE value of 99.998% for Thoroughbred horses. These results indicated that the Microsatellite 15 TKY System is useful for paternity testing of Thoroughbred horses. A paternity testing case for a Thoroughbred horse family, in which candidate sires had close relations, was analyzed using the Microsatellite 15 TKY System. In this case, the Microsatellite 15 TKY System excluded paternity of a false sire. We concluded that the Microsatellite 15 TKY System can give sufficient and reliable information for paternity testing. KEY WORDS: microsatellite, Microsatellite 15 TKY System, paternity testing, PCR, Thoroughbred.

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“…Application of microsatellite loci as forensic genetic markers is common due to their abundance in the genome, extreme polymorphism and amplification by the PCR reaction [8,9]. STR markers can be also used to detect a change in length of a microsatellite allele resulting from either insertion or deletion of repeat units during DNA replication and failure of the DNA mismatch repair system to correct these errors referred to as microsatellite instability (MSI) [10,11].…”

Section: Discussionmentioning

confidence: 99%

Loss of heterozygosity (LOH)--implications for human genetic identification.

Pepiński

Sołtyszewski²,

Skawrońska³

et al. 2009

Folia Histochem Cytobiol.

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Abstract:The aim of this study was assessment of possible effects of loss of heterozygosity on human genetic identification of histolopathogical tissue sections. DNA templates were extracted from tumour tissue specimens excised from oncological patients and from reference blood samples. AmpFlSTR Identifiler PCR Amplification Kit and ABI 310 Genetic Analyzer (Applera) were used to obtain genetic profiles. Frequency of LOH was calculated for respective samples. Fisher's exact test was performed for statistical analysis. Forty-two percent of the 101 cancer cases analysed were found to possess alterations of the microsatellites manifesting with allelic loss. The most frequently altered loci were D3S1358 and D18S51. The alteration was detected in 47% of cases with larynx carcinoma, 44% of cases with uveal melanoma, 60% of cases with cervical cancers, one case of liposarcoma G3 and one case od neurofibrosarcoma. No LOH was found in liposarcoma G1, dermatofibrosarcoma and cystosarcoma protuberans in either primary or recurrent tumours. In benign tumours (lipoma and fibroma) LOH was also absent. During genotyping of DNA extracted from histopathological tissue sections caution should be taken when non-match or exclusion based on few discrepancies is concluded.

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Validation of short tandem repeat analysis for the investigation of cases of disputed paternity

Cited by 60 publications

References 13 publications

Short tandem repeat profiling provides an international reference standard for human cell lines

Short tandem repeat profiling provides an international reference standard for human cell lines

Population Study and Validation of Paternity Testing for Thoroughbred Horses by 15 Microsatellite Loci.

Loss of heterozygosity (LOH)--implications for human genetic identification.

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