Validation study of assay method for DE-310, a novel polymer-bound camptothecin derivative, and the free drug in mouse plasma by liquid chromatography with fluorimetric detection

Quantification of CPT13 in rat plasma using LC–MS/MS for a pharmacokinetic study

“…In recent years, several methods, such as high-performance liquid chromatography (HPLC) with fluorescence detection [9][10][11][12] and mass spectrometry [13][14][15] have been reported for the extraction and detection of CPT and its analogues in biological fluid. Here we describe a sensitive LC-MS/MS to investigate the pharmacokinetic profile of CPT13 in rat plasma using CPT as an internal standard (IS).…”