Validity of using capillary blood for the measurement of plasma ferritin

Pawashe, Aruna B.; Raman, L; Nair, M. G.; Sarma, Janardhana

doi:10.1016/0009-8981(87)90041-6

Cited by 16 publications

(11 citation statements)

References 4 publications

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“…data]. The assay conditions were similar to those adopted for the ELISA of human serum ferritin [17], The monkey serum ferritin ELISA had sensitivity of the order of 2-100 pg/1 with a coefficient of varia tion within the assay less than 5% and a day-to-day variability less than 10%.…”

Section: Methodsmentioning

confidence: 88%

Induction of Dietary Iron Deficiency in Rhesus Monkeys: Sequential Changes in Serum Ferritin and Other Biochemical Indicators of Iron Status

et al. 1994

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The usefulness of serum ferritin as a measure of subclinical stages of iron deficiency has been tested in monkeys by inducing a mild iron deficiency dietarily over a period of 12 months. Various biochemical indicators of iron status were measured periodically along with analysis of liver iron and iron staining of bone marrow samples obtained by biopsy, at the end of the experiment. A mild form of iron deficiency was confirmed by bone marrow staining for iron. Of all the biochemical indicators tested, significant decreases were seen in hemoglobin and hematocrit at the 11th and 10th months, respectively. These changes were consistent with the changes later found in bone marrow grading for iron. Serum ferritin concentration and liver iron concentration did not show any significant difference between the controls and iron-deficient monkeys. Thus, these results do not support the existence of a latent stage of iron deficiency. In the mild form of iron deficiency, the functional compartment, represented by bone marrow iron and hemoglobin, is sensitive to depletion even when there were no changes in storage compartment represented by liver iron and serum ferritin.

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Section: Methodsmentioning

confidence: 88%

Induction of Dietary Iron Deficiency in Rhesus Monkeys: Sequential Changes in Serum Ferritin and Other Biochemical Indicators of Iron Status

et al. 1994

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“…SF and sTfR concentrations were determined by sandwich ELISA systems developed by us. The method adopted for SF estimation was according to the method described by Pawashe et al (1987). The assay was calibrated with human spleen ferritin (WHO, NIBSC, UK).…”

Section: Methodsmentioning

confidence: 99%

Serum transferrin receptor in children with respiratory infections

et al. 2003

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Objective: To study the effect of infection on iron status in children suffering from acute, mild or severe respiratory infections and to determine the nature of anemia in infection using serum transferrin receptor (sTfR) levels. Design: Forty-three children aged between 3 and 5 y with no evidence of infection and receiving iron supplements in the preceding 100 days served as controls. Twenty-one children with mild upper respiratory infection and 94 children hospitalized for acute pneumonia constituted the experimental group. Hemoglobin (Hb), sTfR and serum ferritin were estimated in all the children at the time of diagnosis and again on the 15th and 30th days after the infection in those who were available for followup. Results: Mean (95% CI) sTfR was 6.08 (5.1 -7.1) mg=l in healthy non-anemic children. Upper respiratory infection had no impact on Hb or sTfR but it significantly elevated serum ferritin levels. Eighty-three percent of the children with pneumonia had Hb less than 110 g=l at the time of diagnosis and had elevated mean sTfR, 18.0 (15.7 -20.3) mg=l. There was a decline in mean sTfR by the 15th day of infection to 14.3 (11.3 -17.4) mg=l with further rise to 22.9 (13.0 -31.9) mg=l by 30 days. Serum ferritin was significantly elevated at the time of diagnosis (85.9; 71.1 -100.8 mg=l) as well as at 15 days (89.1; 68 -110.1 mg=l) with a decline by 30 days. Conclusions: Severe lower respiratory infection exaggerates iron-deficient erythropoiesis by blocking release of iron from the storage pools. sTfR may not be a sensitive and specific tool of assessing true iron status of children exposed to severe infections.

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“…Blood samples were collected randomly from 7, 12, and 17 preschoolers when the number of participants in the AWC was >10, >15, and >20, respectively. We assessed iron status of the participants by estimating Hb, serum ferritin (SF), soluble transferrin receptor (sTfR), and hepcidin in blood samples. Ferritin was adjusted for C‐reactive protein (CRP) >5 mg/L by multiplying with 0.67 to adjust for inflammation‐related high iron stores .…”

Section: Methodsmentioning

confidence: 99%

A food synergy approach in a national program to improve the micronutrient status of preschoolers: a randomized control trial protocol

Choudhury

Nair

Balakrishna

et al. 2018

Annals of the New York Academy of Sciences

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Iron deficiency anemia (IDA) is a significant public health issue in India affecting nearly all vulnerable segments of the population. Causes of IDA include low consumption of iron-rich foods combined with poor iron bioavailability of nonheme iron sources. To date, interventions aimed at correcting IDA focus on increasing iron intake through iron supplementation or fortification strategies. In contrast, dietary diversification is a long-term sustainable approach to improve bioavailable iron intake. In this context, the inclusion of vitamin C-rich fruits in the regular diet has proven to improve iron absorption, but the effect on iron status is inconclusive. Considering the ongoing national program for preschoolers in India, we designed a cluster randomized controlled trial (RCT) to test the hypothesis that inclusion of vitamin C-rich fruit in a regular meal would improve iron absorption and lead to better child iron and micronutrient status, cognitive development, gut health, and growth while reducing morbidity. This paper illustrates a context-specific framework and activities to design and functionalize an open-label, three-arm cluster RCT to test a specific hypothesis. The results of this designed trial should generate evidence to inform policy on the effect of a food-based intervention on iron status.

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Validity of using capillary blood for the measurement of plasma ferritin

Cited by 16 publications

References 4 publications

Induction of Dietary Iron Deficiency in Rhesus Monkeys: Sequential Changes in Serum Ferritin and Other Biochemical Indicators of Iron Status

Induction of Dietary Iron Deficiency in Rhesus Monkeys: Sequential Changes in Serum Ferritin and Other Biochemical Indicators of Iron Status

Serum transferrin receptor in children with respiratory infections

A food synergy approach in a national program to improve the micronutrient status of preschoolers: a randomized control trial protocol

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