Valores de referência de parâmetros bioquímicos no sangue de duas linhagens de camundongos

Almeida, Aline Silva; Faleiros, Ana Carolina Guimarães; Teixeira, David Nascimento Silva; Cota, Ulisses Antônio; Chica, Javier Emílio Lazo

doi:10.1590/s1676-24442008000600006

Cited by 21 publications

(13 citation statements)

References 6 publications

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“…The triglyceride levels showed no significant differences between the different groups studied, although the test groups were slightly higher than the casein and also above the reference value for BALB/c and C57BL6 mice, from 21.11 to 29.68 mg.dL -1 as evaluated by Almeida et al (2008) but close to that found by Santos et al (2010) (47.6 mg.dL -1 ). There were statistically significant differences in glucose levels in both groups (Test 1 and Test 2); however, they were lower than that found by Santos et al (2010) (85 mg.dL -1 ).…”

Section: Resultssupporting

confidence: 76%

Annatto seed residue (Bixa orellana L.): nutritional quality

Valério

Ramos

Neto

et al. 2015

Food Sci. Technol (Campinas)

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Considering that annatto seeds are rich in protein, the present work aimed to evaluate the biological quality of this nutrient in the meal residue originating from annatto seed processing. We determined the general composition, mineral levels, amino acid composition and chemical scores, antinutritional factors, and protein quality using biological assays. The following values were obtained: 11.50% protein, 6.74% moisture, 5.22% ash, 2.22% lipids, 42.19% total carbohydrates and 28.45% fiber. The residue proved to be a food rich in fiber and also a protein source. Antinutritional factors were not detected. The most abundant amino acids were lysine, phenylalanine + tyrosine, leucine and isoleucine. Valine was the most limiting amino acid (chemical score 0.22). The protein quality of the seed residue and the isolated protein showed no significant differences. The biological value was lower than that of the control protein but higher than that found in other vegetables. Among the biochemical analyses, only creatinine level was decreased in the two test groups compared to the control group. Enzyme tests did not indicate liver toxicity. The results showed favorable aspects for the use of annatto seed residue in the human diet, meriting further research.Keywords: biological value; protein quality; annatto.Practical Application: The evaluation of biological quality of the annatto seeds' protein showed favorable aspects for the use in the human diet.

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Section: Resultssupporting

confidence: 76%

Annatto seed residue (Bixa orellana L.): nutritional quality

Valério

Ramos

Neto

et al. 2015

Food Sci. Technol (Campinas)

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“…Moreover, among the biochemical parameters studied, only an increase in the urea concentration was detected, but within the normal range for mice (41,97 mg/dl to 60,02 mg/dl), which does not indicate renal toxicity[55]. In fact, compounds like flavonoids, caffeic acid, and their esters present in propolis appear to prevent against membrane fragility in organs such liver and kidney, decreasing the level of urea and the leakage of liver enzymes into the circulation [54,56,57].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity against oral pathogens and immunomodulatory effects and toxicity of geopropolis produced by the stingless bee Melipona fasciculata Smith

Libério

Pereira

Dutra

et al. 2011

BMC Complement Altern Med

104

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BackgroundNative bees of the tribe Meliponini produce a distinct kind of propolis called geopropolis. Although many pharmacological activities of propolis have already been demonstrated, little is known about geopropolis, particularly regarding its antimicrobial activity against oral pathogens. The present study aimed at investigating the antimicrobial activity of M. fasciculata geopropolis against oral pathogens, its effects on S. mutans biofilms, and the chemical contents of the extracts. A gel prepared with a geopropolis extract was also analyzed for its activity on S. mutans and its immunotoxicological potential.MethodsAntimicrobial activities of three hydroalcoholic extracts (HAEs) of geopropolis, and hexane and chloroform fractions of one extract, were evaluated using the agar diffusion method and the broth dilution technique. Ethanol (70%, v/v) and chlorhexidine (0.12%, w/w) were used as negative and positive controls, respectively. Total phenol and flavonoid concentrations were assayed by spectrophotometry. Immunotoxicity was evaluated in mice by topical application in the oral cavity followed by quantification of biochemical and immunological parameters, and macro-microscopic analysis of animal organs.ResultsTwo extracts, HAE-2 and HAE-3, showed inhibition zones ranging from 9 to 13 mm in diameter for S. mutans and C. albicans, but presented no activity against L. acidophilus. The MBCs for HAE-2 and HAE-3 against S. mutans were 6.25 mg/mL and 12.5 mg/mL, respectively. HAE-2 was fractionated, and its chloroform fraction had an MBC of 14.57 mg/mL. HAE-2 also exhibited bactericidal effects on S. mutans biofilms after 3 h of treatment. Significant differences (p < 0.05) in total phenol and flavonoid concentrations were observed among the samples. Signs toxic effects were not observed after application of the geopropolis-based gel, but an increase in the production of IL-4 and IL-10, anti-inflammatory cytokines, was detected.ConclusionsIn summary, geopropolis produced by M. fasciculata can exert antimicrobial action against S. mutans and C. albicans, with significant inhibitory activity against S. mutans biofilms. The extract with the highest flavonoid concentration, HAE-2, presented the highest antimicrobial activity. In addition, a geopropolis-based gel is not toxic in an animal model and displays anti-inflammatory effect.

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“…For this, reference values must be established, with it being necessity to know the individual biochemical parameters of animals used. These references serve as the parameters for evaluating changes in different organs 3 .…”

Section: Introductionmentioning

confidence: 99%

Hematology of Swiss mice (Mus musculus) of both genders and different ages

Restell

Porfírio

Souza³

et al. 2014

Acta Cir. Bras.

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PURPOSE:To describe the hematologic values of male and female, young and adult, Swiss mice (Mus musculus). METHODS:Mus musculus (n=14) were randomly selected and separated by gender. The male and female, young and adult animals were sedation to obtain a blood sample, by intracardiac route at 30, 45, 60, 75, 90, 105 and 120 days after birth. RESULTS:The Swiss mouse hemogram values obtained, in relation to total eosinophils, basophils, and number of platelets, there was no statistical differences according to the genders or the age of the animals. Regarding the erythrocyte, hemoglobin and hematocrit values obtained, these were higher in females. The RDW-CD and MPV values were higher in the females than in the males. CONCLUSIONS:Lymphocytes are the predominant cells in the peripheral blood. The collection of 800 µL of blood by intracardiac route, every 15 days, did not affect the health of the animals. Analyses of the blood samples contribute to the experimental models provided by the Central Animal Facility of UFMS and used by professors.

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Valores de referência de parâmetros bioquímicos no sangue de duas linhagens de camundongos

Abstract: Primeira submissão em 09/10/08 Última submissão em 16/12/08 Aceito para publicação em 20/12/08 Publicado em 20/12/08 artigo origiNal original article

Cited by 21 publications

References 6 publications

Annatto seed residue (Bixa orellana L.): nutritional quality

Annatto seed residue (Bixa orellana L.): nutritional quality

Antimicrobial activity against oral pathogens and immunomodulatory effects and toxicity of geopropolis produced by the stingless bee Melipona fasciculata Smith

Hematology of Swiss mice (Mus musculus) of both genders and different ages

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