Valorization of bioactive compounds from residual saffron biomass (Crocus sativus L.) to obtain high value added dermato-cosmetic products

Abstract

The valorization of indigenous flora waste by extraction of biologically active compounds has potential applications in the medical and cosmetic fields. The polyphenols and flavonoids extracted from this waste are valuable compounds for the manufacture of new cosmetic and/or dermato-cosmetic formulas to protect the skin from oxidative stress. This study obtained plant extracts from saffron waste—petals, tepals, and superior portions of stem—using different solid-liquid extraction techniques. The influence of some physical operating parameters was studied (extraction time, solid/liquid ratio, solvent extraction composition). The extraction method performance was assessed by the value of the extraction yields. The obtained extracts were characterized by the content of polyphenols and flavonoids, and the antioxidant activity determined with the DPPH and ABTS methods and the UV-VIS spectrometry. Some emulsions O/W were prepared and preliminarily characterized (pH, sensory analysis, stability after centrifugation and storage). The obtained results showed that the incorporation of this natural extract did not negatively affect the stability of the studied cosmetic formulations and advanced characterization (microbiological control of contamination, rheology studies and in vitro and in vivo studies) can be continued in order to implement a new product.

Antioxidants from Galium verum as Ingredients for the Design of New Dermatocosmetic Products

“…Extraction was made using conventional and unconventional extraction procedures in the following variants: Heat Reflux Extraction (HTE), room temperature maceration (M), Ultrasound Assisted Extraction (UAE), using a work protocol (extraction time, salt/solvent ratio, and extraction solvent concentrations) established based on our previous studies [ 16 , 17 ] and after some preliminary tests were carried out (data not shown). In all the extraction techniques, 96% ethanol was used to prepare extraction solvent: hydro alcoholic solution with 30%, 50%, 70% v / v concentration.…”

“…To obtain the final emulsion a protocol described by us previously was used [ 17 ] according to which the oily (A) and aqueous (B) phases were heated to 75 °C, after which the oily phase (A) was added over the aqueous phase (B). The mixing of the phases was done using a rotor-stator homogenizer (ESGE Zauberstab M 160 G Gourmet) which operates at 15,000 rpm.…”

Preliminary Approaches to Cosmeceuticals Emulsions Based on N-ProlylPalmitoyl Tripeptide-56 Acetat-Bakuchiol Complex Intended to Combat Skin Oxidative Stress

“…The stability of the prepared emulsions was assessed throughout time as well as potential microbial contamination during storage or usage of dermato-cosmetic preparation [ 37 , 39 , 40 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ].…”

“…The total phenolic content was determined following previously described methods [ 45 , 46 , 47 ]. Briefly, 50 µL of sample were mixed with 100 µL Folin–Ciocalteu reagent and strongly mixed.…”

“…The experiment was carried out using a slightly modified version of a procedure that was previously described [ 45 , 46 , 47 ]. Thus, 50 µL of emulsion extract was added to 150 µL of 2,2-diphenyl-1-picrylhydrazyl (DPPH) 0.004% methanol solution.…”