2021
DOI: 10.3389/fmolb.2021.633315
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Valproic Acid Protects Chondrocytes from LPS-Stimulated Damage via Regulating miR-302d-3p/ITGB4 Axis and Mediating the PI3K-AKT Signaling Pathway

Abstract: Background: Osteoarthritis (OA) is one of the most common degenerative joint diseases characterized by increased apoptosis and autophagy deficiency. The investigation was performed to examine the effect of valproic acid (VPA) and molecular mechanism related to miR-302d-3p/ITGB4 axis in OA.Methods: The OA clinical samples were obtained from the GEO database to analyze differentially expressed genes. An in vitro OA model was mimicked by LPS in CHON-001 cells. Autophagy-related genes were downloaded from the HADb… Show more

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Cited by 18 publications
(14 citation statements)
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“…10 μL CCK-8 reagent was added to each well at indicated time point and incubated in the incubator for 1 hour. The GX71 microplate reader (Olympus Japan) was used to detect the absorbance value (OD value) in each well at 450 nm [ 35 ].…”
Section: Methodsmentioning
confidence: 99%
“…10 μL CCK-8 reagent was added to each well at indicated time point and incubated in the incubator for 1 hour. The GX71 microplate reader (Olympus Japan) was used to detect the absorbance value (OD value) in each well at 450 nm [ 35 ].…”
Section: Methodsmentioning
confidence: 99%
“…Y79 and WERI-Rb1 cells with indicated treatments were seeded into a 96-well plate at a density of 2000 cells/well and cultured in a cell incubator for 0 h, 24 h, 48 h, 72 h. 10 μL CCK8 reaction solution (Solarbio, Beijing, China) was added to each well at indicated time point. The cell culture was incubated in cell incubator for 4 h, and the absorbance at 450 nm was measured by a microplate reader (Bio-Tek Instrument, Winooski) [ 23 ].…”
Section: Methodsmentioning
confidence: 99%
“…SW620 and LOVO cells with B7-H4 overexpression/B7-H4 silencing or the corresponding control cells were trypsinized and seeded in the 96-well-plates (1500 cells/well) and cultured for 0, 24, 48 and 72 h. 10 µl CCK-8 solution (Solarbio, Beijing, China) was added to the cell culture at indicated time point and incubated for 1 h in a humidified cell culture incubator. The light absorption value (OD value) in each condition was captured at 450 nm wavelength on a Synergy H1 microplate reader (Winooski, Vermont, USA) [ 23 ].…”
Section: Methodsmentioning
confidence: 99%