VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of Iran

Saadat, S; Solhjoo, Kavous; Norooz-Nejad, Mohammad-Javad; Kazemi, Akbar

doi:10.5001/omj.2014.90

Cited by 49 publications

(29 citation statements)

References 21 publications

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“…In this study, a positive relation was observed between phenotypic and PCR results for the determination of antimicrobial resistance which is in agreement with Gao et al [29] and Saadat et al [50] . However, Zmantar et al [28] and Salih et al [51] reported no correlation between phenotypic and PCR methods.…”

Section: Tetk-1 Tetksupporting

confidence: 81%

Hayvansal Gıdalardan İzole Edilen Staphylococcus aureus’ların Antibiyotik Dirençlilik Gen Profilleri

Hızlısoy¹,

Onmaz²,

Karadal³

et al. 2018

Kafkas Univ Vet Fak Derg

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In this study, the investigation of the antibiotic resistance gene profiles of Staphylococcus aureus isolates from foods of animal origin was aimed. Totally, 95 S. aureus strains, obtained during a period between 2009 and 2012, from culture collection of the Food Hygiene and Technology Laboratory, were examined. The isolates were confirmed by phenotypic tests and PCR. The antibiotic susceptibilities of the isolates were analyzed by disc diffusion method and the minimal inhibition concentrations of the antibiotics were determined by E test. PCR were also utilized for determining the presence of resistance genes including blaZ, ermA, ermC, tetK, tetM, mecA, VanA, VanB, VatA, VatB and aacA-aphD. Resistance to penicillin, tetracycline, vancomycin, erythromycin, cefoxitin, gentamycin and quinupristin-dalfopristin were evident as 81.1%, 28.4%, 18.9%, 17.9%, 9.4%, 9.4% and 3.2% respectively. E test results were compatible with the disc diffusion method. Multidrug resistance was observed from 29.5% of S. aureus isolates. Positive compatibility was observed between conventional methods and PCR for the resistance of the isolates, except for vancomycin. In addition, all of the tested isolates found to include a resistance gene for at least one antibiotic. In conclusion, more efficient interventions must be followed to control the redundant use of antibiotics in veterinary practice. Furthermore, appropriate control measures are needed to be implemented to reduce contamination and the spread of multiresistant S. aureus strains. Keywords: Antibiotic resistance, Animal origin foods, Multidrug resistance, Resistance genes, Staphylococcus aureus Hayvansal Gıdalardan İzole Edilen Staphylococcus aureus'ların Antibiyotik Dirençlilik Gen Profilleri

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Section: Tetk-1 Tetksupporting

confidence: 81%

Hayvansal Gıdalardan İzole Edilen Staphylococcus aureus’ların Antibiyotik Dirençlilik Gen Profilleri

Hızlısoy¹,

Onmaz²,

Karadal³

et al. 2018

Kafkas Univ Vet Fak Derg

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“…The acquisition and spread of vanZ genes in S. aureus could become a critical problem. The vanZ Tei, gene, as a part of the vanA gene cluster, is occasionally transferred from enterococci into S. aureus, leading to highly vancomycin-resistant strains (VRSA) (Chang et al, 2003;Perichon and Courvalin, 2009;Saadat et al, 2014). Although the incidence of such an event remains low, apparently due to the high fitness cost of vanHAXmediated resistance in S. aureus (Foucault et al, 2009), VRSA strains may represent progenitors for the generation of vanZ Teicarrying mobile genetic elements.…”

Section: Discussionmentioning

confidence: 99%

VanZ Reduces the Binding of Lipoglycopeptide Antibiotics to Staphylococcus aureus and Streptococcus pneumoniae Cells

et al. 2020

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vanZ, a member of the VanA glycopeptide resistance gene cluster, confers resistance to lipoglycopeptide antibiotics independent of cell wall precursor modification by the vanHAX genes. Orthologs of vanZ are present in the genomes of many clinically relevant bacteria, including Enterococcus faecium and Streptococcus pneumoniae; however, vanZ genes are absent in Staphylococcus aureus. Here, we show that the expression of enterococcal vanZ paralogs in S. aureus increases the minimal inhibitory concentrations of lipoglycopeptide antibiotics teicoplanin, dalbavancin, oritavancin and new teicoplanin pseudoaglycone derivatives. The reduction in the binding of fluorescently labeled teicoplanin to the cells suggests the mechanism of VanZ-mediated resistance. In addition, using a genomic vanZ gene knockout mutant of S. pneumoniae, we have shown that the ability of VanZ proteins to compromise the activity of lipoglycopeptide antibiotics by reducing their binding is a more general feature of VanZ-superfamily proteins.

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“…[22] After that, several VRSA isolates confirmed by genetic analysis have been reported from different countries such as US, India, Pakistan, and Iran. [23] In Imam Khomeini Hospital in Tehran, Aligholi et al . identified 2 (0.56%) strains of VRSA confirmed by minimum inhibitory concentration of 64 and 512 mcg/ml for vancomycin as well as detection of vanA gene in 1 of the isolates through polymerase chain reaction (PCR).…”

Section: Discussionmentioning

confidence: 99%

“…[17] Agar screening and PCR tests on 220 staphylococcal samples from three laboratories of Shiraz hospitals (Shahid-Faghihi, Namazi, and MRI) between March and December 2012 demonstrated that vanA and vanB resistant genes were positive in 34% and 37% of clinical isolates, respectively. [23] The development of VRSA could be attributed to overuse and selective pressure of vancomycin. In this regards, results of a cross-sectional study in a referral university-affiliated hospital in Shiraz at 2003 revealed that the prescription of vancomycin was only appropriate in 12 out of 200 (6%) patients according to the Hospital Infection Control Practice Advisory Committee guideline.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance pattern of Gram-positive bacteria during three consecutive years at the nephrology ward of a tertiary referral hospital in Shiraz, Southwest Iran

et al. 2016

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Objective:The aim of the present study was to determine the pattern of antimicrobial resistance of Gram-positive bacteria during three consecutive years at the nephrology ward of Namazi Hospital in Shiraz, Southwest of Iran.Methods:During a 3-year period from 2013 to 2015, data of all biological samples of hospitalized patients at the adult nephrology ward of Namazi Hospital were sent to the central laboratory for identification of Gram-positive microorganisms and subsequently, their antimicrobial susceptibility testing by Kirby–Bauer disc diffusion method were analyzed in a retrospective manner.Findings:Coagulase-negative Staphylococci (CONS) (38.5%), Staphylococcus aureus (25.4%), and Enterococcus spp. (23.8%) were the most common isolated Gram-positive bacteria from all biological samples. All Enterococcus spp. isolates within the 3 years were resistant to oxacillin. The rate of vancomycin-resistant enterococci (VRE) increased from 40.63% in 2013 to 72.73% in 2015. Enterococcus spp. resistance rates to aminoglycosides during 3 years were above 85%. The frequencies of oxacillin-resistant S. aureus (ORSA) in 2013, 2014, and 2015 were 95.24%, 80.95%, and 36.36%, respectively. Two out of 11 (6.67%) S. aureus isolates were resistant to vancomycin. More than 90% of CONS were sensitive to vancomycin within the study period. The frequency of gentamicin-resistant CONS ranged from 40% to 57.14%.Conclusion:The rates of ORSA, VRE, and aminoglycoside-resistant CONS as well as Enterococcus spp. in our clinical setting were considerably high and concerning. These may be due to the failure or lack of infection control activities and antimicrobial selection pressure.

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VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of Iran

Abstract: The results showed that the frequency of vancomycin resistance genes (vanA, vanB) is very high in Staphylococcus aureus strains isolated from patients in south of Iran. Thus, urgent interventions are needed to keep the emergence and transmission of these isolates to a minimum.

Cited by 49 publications

References 21 publications

Hayvansal Gıdalardan İzole Edilen Staphylococcus aureus’ların Antibiyotik Dirençlilik Gen Profilleri

Hayvansal Gıdalardan İzole Edilen Staphylococcus aureus’ların Antibiyotik Dirençlilik Gen Profilleri

VanZ Reduces the Binding of Lipoglycopeptide Antibiotics to Staphylococcus aureus and Streptococcus pneumoniae Cells

Antimicrobial resistance pattern of Gram-positive bacteria during three consecutive years at the nephrology ward of a tertiary referral hospital in Shiraz, Southwest Iran

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