Vanadate monomers and dimers both inhibit the human prostatic acid phosphatase

Crans, Debbie C.; Simone, Carmen M.; Saha, Asish K.; Glew, Robert H.

doi:10.1016/0006-291x(89)91061-9

Cited by 46 publications

(29 citation statements)

References 9 publications

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“…Vanadate is a broad-spectrum phosphatase inhibitor, although its action is more potent on tyrosine phosphatases than on serine and threonine phosphatases (Crans et al, 1989;Huyer et al, 1997). To confirm whether mangiferin inhibits ATR phosphorylation, HL-60 cells were exposed to mangiferin for the indicated times.…”

Section: Discussionmentioning

confidence: 99%

Mangiferin induces cell cycle arrest at G2/M phase through ATR-Chk1 pathway in HL-60 leukemia cells

Peng¹,

Yao²,

Yang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. This study aimed to determine the effect of mangiferin on the cell cycle in HL-60 leukemia cells and expression of the cell cycle-regulatory genes Wee1, Chk1 and CDC25C and to further investigate the molecular mechanisms of the antileukemic action of mangiferin. The inhibitory effect of mangiferin on HL-60 leukemia cell proliferation was determined by the MTT assay. The impact of mangiferin on the HL-60 cell cycle was evaluated by flow cytometry. After the cells were treated with different concentrations of mangiferin, the expression levels of Wee1, Chk1 and CDC25C mRNA were determined by RT-PCR, and Western blot was used to evaluate the expression levels of cdc25c, cyclin B1, and Akt proteins. The inhibition of HL-60 cell growth by mangiferin was dose-and time-dependent. After treatment for 24 h, cells in G2/M phase increased, and G2/M phase arrest appeared with increased mRNA expression of Wee1, Chk1 and CDC25C. Mangiferin inhibited Chk1 and cdc25c mRNA expression at high concentrations and induced Wee1 mRNA expression in a dose-dependent manner. It significantly inhibited ATR, Chk1, Wee1, Akt, and ERK1/2 phosphorylation but increased cdc2 and cyclin Z.G. Peng et al. 4990©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 4989-5002 (2015) B1 phosphorylation. Furthermore, mangiferin reduced cdc25c, cyclin B1, and Akt protein levels while inducing Wee1 protein expression. It also antagonized the phosphorylation effect of vanadate on ATR, and the phosphorylation effect of EGF on Wee1. These findings indicated that mangiferin inhibits cell cycle progression through the ATR-Chk1 stress response DNA damage pathway, leading to cell cycle arrest at G2/M phase in leukemia cells.

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Section: Discussionmentioning

confidence: 99%

Mangiferin induces cell cycle arrest at G2/M phase through ATR-Chk1 pathway in HL-60 leukemia cells

Peng¹,

Yao²,

Yang³

et al. 2015

Genet. Mol. Res.

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“…In Fig. 16a, the correlation is shown of the inhibition of wheat germ acid phosphatase with V 1 [163]. Since the correlation is linear when plotting the reciprocal rate of substrate hydrolysis as a function of concentration of V 1 , the inhibition is attributed to the monomer.…”

Section: What Are the Effects Of Vanadium Species In Biological Systems?mentioning

confidence: 98%

Fifteen years of dancing with vanadium

Crans

2005

Pure and Applied Chemistry

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“…1) is a more potent inhibitor than the vanadate (V 1 ) was reported for 6-phosphogluconate dehydrogenase [63] almost two decades ago. This investigation was followed up with a study of glucose-6-phosphate dehydrogenase [81] and a number of other enzymes [4,[82][83][84][85][86]. Since then many observations have followed, documenting the specific interactions between various proteins and different oxovanadate anions [4,27,45].…”

Section: Decavanadate Interactions and Effects In Proteinsmentioning

confidence: 99%