Vancomycin and High Level Aminoglycoside Resistance in<i>Enterococcus</i>spp. in a Tertiary Health Care Centre: A Therapeutic Concern

Mittal, Sakshi; Singla, Pooja; Deep, Antariksh; Bala, Kiran; Sikka, Rama; Garg, Meenu; Chaudhary, Uma

doi:10.1155/2016/8262561

Cited by 22 publications

(13 citation statements)

References 18 publications

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“…In general, the prevalence of VRE is as low as as 4% in Europe and as high as 33% in North America. In India, the prevalence of VRE ranges from 0 to 30 % [7,8]. As a result, Enterococci have emerged as one of the leading therapeutic challenges when identified as a cause of serious; life threatening infections.…”

Section: Introductionmentioning

confidence: 99%

Incidence and Antimicrobial Susceptibility of Enterococcal Infections in Tertiary Care Hospital

Angadi¹,

Jadhav²,

Mirza³

et al. 2018

BIOP

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Section: Introductionmentioning

confidence: 99%

Incidence and Antimicrobial Susceptibility of Enterococcal Infections in Tertiary Care Hospital

Angadi¹,

Jadhav²,

Mirza³

et al. 2018

BIOP

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“…In this study, a high rate of resistance to fosfomycin was observed, while this antibiotic is as an alternative antibiotic against multidrug resistant organisms, including vancomycinresistant enterococcus (VRE) and extendedspectrum β-lactamase (ESBL) (47) which could be due to its excessive and inappropriate use. In addition, according to many reported studies (48)(49)(50), E. faecalis and E. faecium isolates exhibit high resistance to aminoglycosides including gentamycin and quinupristin/dalfopristin. Our findings showed that 21% of E. faecalis and 35% of E. faecium isolates are resistant to gentamycin while a high number of isolates were resistant to quinupristin/dalfopristin (81% and 79%, respectively).…”

Section: Discussionmentioning

confidence: 99%

Assessment of Susceptibility to Five Common Antibiotics and Their Resistance Pattern in Clinical Enterococcus Isolates

Zavaryani

Mirnejad

Piranfar³

et al. 2020

Iran J Pathol

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KEYWORDS ABSTRACTEnterococcus faecalis, Enterococcus faecium, Multiple drug resistance, Correlation Scan to discover online Background & Objective: Enterococcus Species are the common cause of nosocomial infections, which are highly resistant to different antibiotics. Therefore, determination of their antibiotic susceptibility patterns and simultaneous resistance to antibiotics is important for better treatment strategies. Methods: 400 clinical Enterococcus isolates were collected from different hospitals in Tehran, Iran. Standard phenotypic-biochemical tests and PCR were used to identify the Enterococcus species. The antimicrobial susceptibility patterns and simultaneous resistance to selected antibiotics were determined by disk diffusion method according to the CLSI guidelines. All data analysis was performed using Python packages Scipy and Stats models. Results: According to the biochemical and PCR analyses, among 400 Enterococcus species, 72% of samples were Enterococcus faecalis, 10.75% Enterococcus faecium, and 17.25% other Enterococcus species.The results determined antimicrobial resistances of these strains against gentamicin, vancomycin, fosfomycin trometamol, teicoplanin, and quinupristin/dalfopristin. Results confirmed a significant correlation between resistance to vancomycin and resistance to teicoplanin. This correlation remains significant when including only E. faecium or E. faecalis species. We also found a negative correlation between resistance to teicoplanin and quinupristin/dalfopristin. Additionally, Quinupristin/dalfopristin was the least effective antibiotic while vancomycin and teicoplanin were the most effective ones.Conclusion: Based on the results and association between simultaneous resistance to some antibiotics such as vancomycin and teicoplanin, in the case of antibiotic resistance, the choice of a second antibiotic can be very important which can lead to good or bad effects. Main Subjects:Microbiology

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“…The isolates which show intermediate zone (7-9 mm) and resistance zone (<6 mm) to 120 µg disc were further tested for MIC by E-strip method. 7,8 MIC determination were done by using gentamicin Ezy MICTM (HiMedia) strip containing gradient concentration from 0.064 to 1024 mcg/ml. MIC >500 mcg/ml is considered as HLAR.…”

Section: Detection Of High-level Aminoglycoside Resistance (Hlar)mentioning

confidence: 99%

Detection of various virulence factors in high level aminoglycoside resistance and vancomycin resistant enterococci isolates of uropathogenic Enterococci

et al. 2019

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Background: Enterococci are common commensal organism of enteric tract and act as opportunistic pathogen and may cause infection in community as well as in hospitalised individuals. In present study association of several types of virulence factors like haemolysin, gelatinase and biofilm formation have been studied among HLAR and Vancomycin resistant Enterococci (VRE) isolates of enterococci among UTI patients.Methods: The samples were collected from all hospitalized and OPD patients of MBS Hospital, JK Lone Hospital and NMC Hospital. Government Medical College, Kota, Rajasthan, India. A total of 360 isolates of enterococcus were collected during the period of 2 years from April 2016 to April 2018 in microbiology laboratory, Department of Microbiology, Government Medical College, Kota, Rajasthan, India. All virulence factors were detected by phenotypic methods and MIC values were detected for high level gentamicin and vancomycin.Results: Among all enterococcal isolates most common factor was biofilm production 191 (53.05%) followed by haemolysin 131 (36.38%) and gelatinase production 72 (20%). Total resistant (MIC> 500 µg/ml) isolates for gentamicin was 194 (89.4%). In agar dilution 14 (11.2%) isolates were found sensitive, 61 (48.8%) isolates were found intermediate and 50 (40%) isolates were found to be resistant for vancomycin. HLAR and VRE was maximum associated with haemolysin + bio-film followed by gelatinase+biofilm, haemolysin+gelatinase+bio- film and least with haemolysin + gelatinase.Conclusions: In present study enterococcus show significant production of biofilm and other virulence factors. With production of biofilm they become more resistant to routinely used concentration of antibiotics posing threat for treatment failure. A continuous monitoring is needed particularly for resistance to aminoglycoside and vancomycin to stop their institutional spread. Judicial use of antibiotics should be encouraged both in community as well as in institutions.

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Vancomycin and High Level Aminoglycoside Resistance inEnterococcusspp. in a Tertiary Health Care Centre: A Therapeutic Concern

Cited by 22 publications

References 18 publications

Incidence and Antimicrobial Susceptibility of Enterococcal Infections in Tertiary Care Hospital

Incidence and Antimicrobial Susceptibility of Enterococcal Infections in Tertiary Care Hospital

Assessment of Susceptibility to Five Common Antibiotics and Their Resistance Pattern in Clinical Enterococcus Isolates

Detection of various virulence factors in high level aminoglycoside resistance and vancomycin resistant enterococci isolates of uropathogenic Enterococci

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