Vancomycin functionalized WO3 thin film-based impedance sensor for efficient capture and highly selective detection of Gram-positive bacteria

Singh, Sanjay; Moudgil, Akshay; Mishra, Nishant; Das, Samaresh; Mishra, Prashant

doi:10.1016/j.bios.2019.04.029

Cited by 52 publications

(20 citation statements)

References 63 publications

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“…design by Singh and coworkers, vancomycin (Van), which usually binds to only Gram-positive bacteria, was functionalized on the surface of tungsten oxide (WO 3 ) interdigitated gold electrodes immobilized on a silicon substrate. [46] This device demonstrated similar LOD as with the use of antibodies, hence confirming the benefits of immunosensing in bacterial detection.…”

Section: Impedance-based Biosensorssupporting

confidence: 57%

Biosensors and Point‐of‐Care Devices for Bacterial Detection: Rapid Diagnostics Informing Antibiotic Therapy

Gopal

Kashif

et al. 2021

Adv Healthcare Materials

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With an exponential rise in antimicrobial resistance and stagnant antibiotic development pipeline, there is, more than ever, a crucial need to optimize current infection therapy approaches. One of the most important stages in this process requires rapid and effective identification of pathogenic bacteria responsible for diseases. Current gold standard techniques of bacterial detection include culture methods, polymerase chain reactions, and immunoassays. However, their use is fraught with downsides with high turnaround time and low accuracy being the most prominent. This imposes great limitations on their eventual application as point-of-care devices. Over time, innovative detection techniques have been proposed and developed to curb these drawbacks. In this review, a systematic summary of a range of biosensing platforms is provided with a strong focus on technologies conferring high detection sensitivity and specificity. A thorough analysis is performed and the benefits and drawbacks of each type of biosensor are highlighted, the factors influencing their potential as point-of-care devices are discussed, and the authors' insights for their translation from proof-of-concept systems into commercial medical devices are provided.

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Section: Impedance-based Biosensorssupporting

confidence: 57%

Biosensors and Point‐of‐Care Devices for Bacterial Detection: Rapid Diagnostics Informing Antibiotic Therapy

Gopal

Kashif

et al. 2021

Adv Healthcare Materials

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“…[ 1 ] It is effective against Gram-positive bacteria by disrupting cell wall synthesis and has been approved for clinical use for more than 60 years. [ 2 , 3 ] It is commonly used for methicillin-resistant Staphylococcus aureus (MRSA), ampicillin-resistant enterococci and Gram-positive organisms in patients allergic to penicillin. [ 4 – 6 ] Vancomycin is usually given by intravenous drip.…”

Section: Introductionmentioning

confidence: 99%

Adverse reactions of vancomycin in humans

Yang

et al. 2020

Medicine

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Background: Vancomycin is effective against Gram-positive bacteria and considered as a last resort in the case of ineffective use of other antigens. While due to the occurrence of adverse reactions, the application of vancomycin is strictly limited. We will conduct a meta-analysis to summarize adverse reactions of vancomycin in humans. Methods: To collect comprehensive randomized controlled trials (RCTs), the following electronic databases will be searched: PubMed, Embase, Web of Science, Cochrane Library, the China National Knowledge Infrastructure, Chinese Biomedical Literature Database, and China Science and Technology Journal Database. The range of publication time will be from the inception of the database to August 2020 without language limitation. Two reviewers will independently conduct selection of studies, data extraction and management, and assessment of risk of bias. Any disagreement will be resolved by discussion with the third reviewer. Review Manager 5.3 (The Nordic Cochrane Centre, The Cochrane Collaboration) will be used for meta-analysis. The Cochrane risk of bias tool will be used to assess the risk of bias. Results: This study will synthesize the data from the present eligible high quality RCTs to explore the incidence of adverse reactions such as hypersensitivity reactions, nephrotoxicity, ototoxicity, phlebitis, and agranulocytosis. Conclusion: This meta-analysis will provide systematic evidence for adverse reactions of vancomycin in humans. Study registration number: INPLASY202080094

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“…Moudgil et al developed this hybrid FET, which used immobilized vancomycin, an antibiotic, to bind to peptidoglycans on the cell wall of the bacteria. 181 When the S. aureus bacteria, which are negatively charged due to anionic lipoteichoic acids and lipopolysaccharides in the cell wall, 182 are captured by vancomycin, the current in the channel decreases due to a reduction in charge carriers. The sensor was highly sensitive, with a LOD of 50 CFU/mL and was reported to distinguish between samples of live and dead bacteria.…”

Section: ■ Chromatography Techniquesmentioning

confidence: 99%