Vancomycin pre-treatment impairs tissue healing in experimental colitis: Importance of innate lymphoid cells

Zhao, Di; Cai, Chenwen; Zheng, Qing; Jin, Shuang; Song, Dongjuan; Shen, Jun; Ran, Zhihua

doi:10.1016/j.bbrc.2016.12.160

Cited by 9 publications

(12 citation statements)

References 26 publications

(23 reference statements)

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“…Son et al found that oestrogen upregulation abrogates experimental OA via the oestrogen-related receptor family of orphan nuclear receptors [64]. A lack of oestrogens increases subchondral bone remodelling, and such bone remodelling has been suggested to ultimately induce osteoarthritic dysbiosis [65].…”

Section: Effect Of Gut Microbiota Dysbiosis On Oa Network Correlationmentioning

confidence: 99%

Gut microbiome dysbiosis alleviates the progression of osteoarthritis in mice

et al. 2020

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Gut microbiota dysbiosis has been studied under the pathological conditions of osteoarthritis (OA). However, the effect of antibiotic-induced gut flora dysbiosis on OA remains incompletely understood at present. Herein, we used a mouse (8 weeks) OA model of destabilization of the medial meniscus (DMM) and gut microbiome dysbiosis induced by antibiotic treatment with ampicillin and neomycin for 8 weeks. The results show that antibiotic-induced intestinal microbiota dysbiosis reduced the serum level of lipopolysaccharide (LPS) and the inflammatory response, such as suppression of the levels of tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6), which can lead to decreased matrix metalloprotease-13 (MMP-13) expression and improvement of OA after joint injury. In addition, trabecular thickness (Tb.Th) and osteophyte scores were increased significantly in antibiotic-induced male mice compared with female mice. We further used network correlation analysis to verify the effect of gut microbiota dysbiosis on OA. Therefore, this study contributes to our understanding of the gut-joint axis in OA and reveals the relationship between the inflammatory response, sex and gut microbiota, which may provide new strategies to prevent the symptoms and long-term sequelae of OA.

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Section: Effect Of Gut Microbiota Dysbiosis On Oa Network Correlationmentioning

confidence: 99%

Gut microbiome dysbiosis alleviates the progression of osteoarthritis in mice

et al. 2020

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“…The results of these studies support the growing idea that changes to the gut microbiome, whether a cause or a consequence of CRC, are related to the disease. Antibiotics can be useful tools for determining how certain species or communities of bacteria affect gut health by altering the composition of the intestinal microbial community [17][18][19][20][21][22][23][24][25][26]. Oral administration of the antibiotic vancomycin has been used to evaluate the impact of changes that occur in the gut microbiome in chronic disease models because it is not absorbed systemically and its effects are localized to the gut.…”

Section: Introductionmentioning

confidence: 99%

“…Oral administration of the antibiotic vancomycin has been used to evaluate the impact of changes that occur in the gut microbiome in chronic disease models because it is not absorbed systemically and its effects are localized to the gut. Vancomycin treatment in pre-clinical CRC models has led to changes in the microbiome and the severity of CRC [26,27]. However, these studies have had equivocal results.…”

Section: Introductionmentioning

confidence: 99%

The Western Dietary Pattern Combined with Vancomycin-Mediated Changes to the Gut Microbiome Exacerbates Colitis Severity and Colon Tumorigenesis

et al. 2021

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Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development. Others have also shown that vancomycin-mediated changes to the gut microbiome increased colorectal cancer (CRC). Therefore, the objective of this study was to determine the impact of vancomycin on colon tumorigenesis in the context of a standard mouse diet or the TWD. A 2 × 2 factorial design was used, in which C57Bl/6J mice were fed either the standard AIN93G diet or TWD and with vancomycin in the drinking water or not. While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments. Vancomycin treatment significantly decreased alpha diversity and changed the abundance of several taxa at the phylum, family, and genus levels. Conversely, basal diet had relatively minor effects on the gut microbiome composition. These results support our previous research that the TWD promotes colon tumorigenesis and suggest that vancomycin-induced changes to the gut microbiome are associated with higher tumor rates.

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“…Moreover, antibiotics alter the expression of genes related to the cell cycle leading to the reduction of epithelial activity in colonic epithelium suggesting that commensal microbiota drive colonic epithelial proliferation (Reikvam et al, ). In addition, mice treated with VCM alone exhibited delayed recovery from DSS‐induced colitis due to a lack of IL‐22 required for wound healing of colonic epithelial cells (Zhao et al, ). Despite the breadth of this research, the precise mechanisms underlying colitogenic effects of antibiotics are still largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Oral administration of antibiotics results in fecal occult bleeding due to metabolic disorders and defective proliferation of the gut epithelial cell in mice

et al. 2018

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Antibiotics sometimes exert adverse effects on the pathogenesis of colitis due to the dysbiosis resulting from the disruption of gut homeostasis. However, the precise mechanisms underlying colitogenic effects of antibiotic‐induced colitis are largely unknown. Here, we show a novel murine fecal occult bleeding model induced by the combinatorial treatment of ampicillin and vancomycin, which is accompanied by an enlarged cecum, upregulation of pro‐inflammatory cytokines IL‐6 and IL‐12, a reduction in Ki‐67‐positive epithelial cell number and an increase in the apoptotic cell number in the colon. Moreover, gas chromatography–tandem mass analysis showed that various kinds of metabolites, including glutamic acid and butyric acid, were significantly decreased in the cecal contents. In addition, abundance of butyric acid producer Clostridiales was dramatically reduced in the enlarged cecum. Interestingly, supplementation of monosodium glutamate or its precursor glutamine suppressed colonic IL‐6 and IL‐12, protected from cell apoptosis and prevented fecal occult blood indicating that the reduced level of glutamic acid is a possible mechanism of antibiotic‐induced fecal occult bleeding. Our data showed a novel mechanism of antibiotic‐induced fecal occult bleeding providing a new insight into the clinical application of glutamic acid for the treatment of antibiotic‐induced colitis.

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Vancomycin pre-treatment impairs tissue healing in experimental colitis: Importance of innate lymphoid cells

Cited by 9 publications

References 26 publications

Gut microbiome dysbiosis alleviates the progression of osteoarthritis in mice

Gut microbiome dysbiosis alleviates the progression of osteoarthritis in mice

The Western Dietary Pattern Combined with Vancomycin-Mediated Changes to the Gut Microbiome Exacerbates Colitis Severity and Colon Tumorigenesis

Oral administration of antibiotics results in fecal occult bleeding due to metabolic disorders and defective proliferation of the gut epithelial cell in mice

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