Vancomycin sensitivity of Staphylococcus aureus isolates from hospital patients in Karachi, Pakistan

Hakim, Shazia Tabassum; Arshed, Sabrina; Iqbal, M; Javaid, SG

doi:10.3402/ljm.v2i4.4728

Cited by 15 publications

(15 citation statements)

References 18 publications

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“…The present study encountered one VRSA strain (0.8%) with VA MIC 32µg/ml. Many studies encountered VRSA strains at higher frequencies than that reported in our study; Saderi et al 51 reported that 3.5% of SA isolates were VRSA and Hakim et al 52 reported VISA (13%). On the other hand, Dhanalakshmi, et al 24 reported that no VISA or VRSA were found among MRSA strains.…”

Section: Discussioncontrasting

confidence: 48%

Prevalence and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus in an Egyptian University Hospital

Taha¹,

Badr²,

El-Morsy³

et al. 2019

J Pure Appl Microbiol

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The relative high burden of morbidity and mortality caused by Staphylococcus aureus (SA) in healthcare and community settings is a major concern worldwide. It can cause invasive infections, sepsis and deaths. Despite progress in methicillin-resistant S. aureus (MRSA) prevention in healthcare settings, there is a critical need for assessment of the problem in both healthcare and community settings. This study was conducted for examining the prevalence, risk factors and antimicrobial susceptibility of MRSA in Mansoura University Hospitals (MUHs), Egypt. Samples were collected from patients in MUHs with clinically suspected nosocomial infections. MRSA isolates were identified by the standard bacteriological methods, biochemical reactions and disc diffusion method as recommended by the Clinical & Laboratory Standards Institute (CLSI), then confirmed by MecA gene PCR. A total of 2006 isolates was obtained. SA (32%) was the most frequently isolated pathogen. MRSA (130 isolates) represented 20% of SA and 6.48 % of all isolates. The mecA PCR identified SA as MRSA in 99.2% of cases. MRSA was isolated with another organism (mostly Gram-negative bacilli) from 40.8% of cases while 59.2% of MRSA was isolated alone. The most important reported risk factors for MRSA infections were prolonged hospital stays, recent antibiotic therapy, ICU admission, indwelling devices and presence of surgical sutures. MRSA was resistant to many antibiotics but sensitive to vancomycin in 99.2% of cases. Minimizing exposure to the risk factors with rapid diagnosis of MRSA infections are essential for early initiation of appropriate antibiotic treatment and limitation of the non-optimal use of glycopeptides and deaths.

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Section: Discussioncontrasting

confidence: 48%

Prevalence and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus in an Egyptian University Hospital

Taha¹,

Badr²,

El-Morsy³

et al. 2019

J Pure Appl Microbiol

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“…The MIC profile revealed 7 (8.97 %) MRSA isolates as VISA. A previous study conducted at Pakistan also reported 13 % frequency of VISA using MIC by E test (Hakim et al 2007 ). In the current study, we observed 15.84 % inducible clindamycin resistance in MRSA isolates which is in line with other studies (Yilmaz et al 2007 ; Maninder et al 2013 ).…”

Section: Discussionmentioning

confidence: 87%

High frequency of methicillin-resistant Staphylococcus aureus in Peshawar Region of Pakistan

et al. 2016

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Staphylococcus aureus is an increasing problem in clinical practice because of reduced susceptibility to available antibiotics. The objective of the study was to determine the frequency of Methicillin-resistant S.aureus (MRSA) in Peshawar, Pakistan. Clinical isolates of S. aureus were subjected to determination of antibiotic resistance, MICs and inducible clindamycin resistance (ICR). Out of total 280 S. aureus isolates, the frequency of MRSA was 36.1 % (n = 101). MRSA infection was found higher among the age group 50–59 years (60.71 %, OR 3.09), followed by 20–29 years (47.5 %, OR 1.74). Frequency of MRSA in female and male was 39.8 and 34 % respectively. MRSA was more frequent in blood specimens (48.7 %, OR 2.14). The frequency of community and hospital acquired MRSA was 42 and 34.8 % respectively. MRSA showed high resistance (100 %) to penicillin and cefoxitin followed by erythromycin (99 %). While MRSA exhibited 100 % susceptibility to vancomycin and linezolid. We have also found 7 vancomycin intermediate sensitive S. aureus (VISA) isolates. ICR was observed in 15.84 % (n = 16) of MRSA isolates. It is concluded that MRSA is potential threat to public health in Peshawar. Vancomycin and linezolid could be prescribed as a drug of choice in treating MRSA associated infections. In addition, ICR should be routinely checked to avoid clindamycin treatment failure.

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“…MIC 50 MIC 90 0.19 to 3 µg/ml 0.75 µg/ml 1.5 µg/ml strains were detected in a study carried out in King Edward Medical College Lahore in 2004. In the year 2007, 13% VISA strains were detected from 2 civil hospitals of Karachi but no VRSA strain was detected (Mehmood et al, 2007;Bukhari et al, 2004;Hakim et al, 2007). Regional studies show following VISA incidence, 3.3% in Srinagar Kashmir 2003, 6% in India 2007and 7.5% in Iran 2008(Assadullah et al, 2003Mehdinejad et al, 2008;Horieh et al, 2005).…”

Section: Rangementioning

confidence: 99%

Current status of vancomycin susceptibility against methicillin resistant Staphylococcus aureus (MRSA) strains: A study at two tertiary care hospitals of Pakistan

Kaleem¹,

Usman²,

Sattar³

et al. 2012

Afr. J. Microbiol. Res.

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Vancomycin has been considered mainstay treatment of infections caused by methicillin-resistant Staphylococcus aureus. The reports of the emergence of vancomycin intermediate and vancomycin resistant S. aureus from various parts of the world have been of great clinical concern. This study was performed to monitor the status of glycopeptide susceptibility against methicillin resistant S. aureus in our set up. All non-duplicate methicillin resistant Staphylococcus aureus (MRSA) isolates recovered during the period of study from various wards of Military Hospital Rawalpindi and PAEC General Hospital Islamabad, were subjected to the detection of minimum inhibitory concentrations of vancomycin using E-strips. Results were analyzed to evaluate the possible presence of vancomycin intermediate and resistant strains in the set up. A total of 276 methicillin-resistant S. aureus isolates were studied. The range of vancomycin minimum inhibitory concentrations (MIC) was 0.19 to 3 ug/mL. MIC 50 came out to be 0.75 ug/mL whereas the MIC 90 was 1.5 ug/ mL. 128 out of 276 (46%) isolates had vancomycin MIC equal to or greater than 1 ug/mL. Majority of the isolates (69%) were from pus samples. No vancomycin resistant or intermediate strain of MRSA was isolated during the study but there were a significant number of isolates having ≥ 1 µg/ml MIC of vancomycin.

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Vancomycin sensitivity of Staphylococcus aureus isolates from hospital patients in Karachi, Pakistan

Cited by 15 publications

References 18 publications

Prevalence and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus in an Egyptian University Hospital

Prevalence and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus in an Egyptian University Hospital

High frequency of methicillin-resistant Staphylococcus aureus in Peshawar Region of Pakistan

Current status of vancomycin susceptibility against methicillin resistant Staphylococcus aureus (MRSA) strains: A study at two tertiary care hospitals of Pakistan

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