Variability in Galactomannan detection by platelia Aspergillus EIA™ according to the Aspergillus species

Xavier, Melissa Orzechowski; Araujo, Juliana S. V.; Aquino, Valério Rodrigues; Severo, Cecília Bittencourt; Guazzelli, Luciana Silva; Severo, Luiz Carlos; Pasqualotto, Alessandro C.

doi:10.1590/s0036-46652013000300001

Cited by 11 publications

(7 citation statements)

References 9 publications

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“…Since Aspergillus hyphae release far more GM than Aspergillus conidia, strain-related differences in germination could explain these findings 11,12 . Differences in GM release have already been described between and within Aspergillus species, including in the same strains of A. fumigatus used in our study 13,14 .…”

Section: Gm Indexsupporting

confidence: 63%

Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?

Cabana

Mendes

Klafke

et al. 2018

Rev. Soc. Bras. Med. Trop.

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Section: Gm Indexsupporting

confidence: 63%

Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?

Cabana

Mendes

Klafke

et al. 2018

Rev. Soc. Bras. Med. Trop.

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“…However, restriction to a specific species decreases the risk of PCR positivity from environmental non- fumigatus DNA, especially if high C q are to be considered ( Alanio and Bretagne, 2014 ), although contamination with A. fumigatus DNA cannot be completely excluded ( Harrison et al, 2010 ; Millon et al, 2010 ). Moreover, since all the Aspergillus species do not equally produced GM in the same quantity ( Xavier et al, 2013 ), quantitative comparison between GM and circulating DNA would have been biased if a non- A. fumigatus specific PCR had been used.…”

Section: Discussionmentioning

confidence: 99%

Circulating Aspergillus fumigatus DNA Is Quantitatively Correlated to Galactomannan in Serum

et al. 2017

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The performance of antigen galactomannan (GM) for diagnosing invasive aspergillosis (IA) is hampered by the occurrence of false-positive results. Quantitative PCR has been proposed to improve the diagnosis of IA. Therefore, we analyzed the value of performing a PCR test to the GM-positive serum sample. Using a quantitative PCR assay specific for Aspergillus fumigatus 28S ribosomal DNA, we retrospectively tested 422 GM-positive (Platelia Bio-Rad kit) serum samples collected over 1 year from 147 patients. The cases were classified based on EORTC criteria as “proven,” “probable,” and “no–IA” before availability of the PCR results. After exclusion of 65 samples for non-reproducibility of GM positivity (n = 62) or PCR inhibition (n = 3), 75 (21.0%) of the remaining 357 samples were PCR-positive. GM and fungal DNA showed a significantly positive correlation (p < 0.0001, R2 = 0.27, slope = 0.98 ± 0.19). At least one PCR-positive result was observed in 63.3% (31/49) of IA patients and in 13.2% (13/98) of non-IA patients (p < 0.0001). The PCR positivity was also associated with the presence of other microbiological criteria among the 44 patients with IA and complete mycological workup (p = 0.014), as well as a higher mortality rate at six months among the 135 patients with hematological conditions (p = 0.0198). Overall, we found a quantitative correlation between serum GM and circulating DNA with an increased likelihood of IA when both were positive. A PCR-positive result also supported a higher fungal load when GM was already positive. We advocate adding a PCR test for every confirmed GM-positive serum sample.

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“…To the best of our knowledge, none of the previous studies reported a twofold mean of the GM index in IA patients with A. fumigatus in comparison to IA patients with A. flavus. Xavier et al [53] in an in vitro study found that the quantity of scattered GM varies according to the species of Aspergillus. They demonstrated that among different species of Aspergillus, Aspergillus terreus has the maximum GM index, closely followed by A. fumigatus in comparison to Aspergillus niger and A. flavus [53].…”

Section: Discussionmentioning

confidence: 98%

“…Xavier et al [53] in an in vitro study found that the quantity of scattered GM varies according to the species of Aspergillus. They demonstrated that among different species of Aspergillus, Aspergillus terreus has the maximum GM index, closely followed by A. fumigatus in comparison to Aspergillus niger and A. flavus [53]. Another study showed a higher secretion of GM by A. niger and A. flavus in comparison to A. fumigatus [54].…”

Section: Discussionmentioning

confidence: 98%

Effect of involved Aspergillus species on galactomannan in bronchoalveolar lavage of patients with invasive aspergillosis

Armaki

Hedayati

Moqarabzadeh

et al. 2017

Journal of Medical Microbiology

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Purpose. The detection of galactomannan (GM) in bronchoalveolar lavage (BAL) fluid is an important surrogate marker for the early diagnosis and therapeutic monitoring of invasive aspergillosis (IA), regardless of the involved species of Aspergillus. Here, we utilized the Platelia Aspergillus GM enzyme immunoassay (Bio-Rad) to evaluate the GM index in BAL fluid samples from patients with proven, probable or putative IA due to Aspergillusflavus versus Aspergillusfumigatus.Methodology. In a prospective study between 2009 and 2015, 116 BAL samples were collected from suspected IA patients referred to two university hospitals in Tehran, Iran.Key findings. According to European Organization for Research and Treatment of Cancer and Mycoses Study Group and Blot criteria, 35 patients were classified as IA patients, of which 33 cases tested positive for GM above 0.5 and, among these patients, 22 had a GM index !1. Twenty-eight were culture positive for A. flavus and seven for A. fumigatus. The GM index for A. flavus cases was between 0.5-6.5 and those of A. fumigatus ranged from 1 to 6.5. The sensitivity and specificity of a GM index !0.5 in cases with A. flavus were 86 and 88 % and for A. fumigatus patients were 100 and 73 %, respectively.Conclusion. Overall, the mean GM index in patients with A. fumigatus (3.1) was significantly higher than those of A. flavus (1.6; P-value=0.031) and the sensitivity of GM lower for A. flavus when compared to A. fumigatus. This finding has implications for diagnosis in hospitals and countries with a high proportion of A. flavus infections.

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Variability in Galactomannan detection by platelia Aspergillus EIA™ according to the Aspergillus species

Cited by 11 publications

References 9 publications

Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?

Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?

Circulating Aspergillus fumigatus DNA Is Quantitatively Correlated to Galactomannan in Serum

Effect of involved Aspergillus species on galactomannan in bronchoalveolar lavage of patients with invasive aspergillosis

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