Variability in seeds’ physicochemical characteristics, germination and seedling growth within and between two French Populus nigra L. populations

Lefebvre, Marlène; Villar, Marc; Boizot, Nathalie; Delile, Armelle; Dimouro, Benjamin; Lomenech, Anne-Marie; Teyssier, Caroline

doi:10.24072/pcjournal.86

Cited by 4 publications

(5 citation statements)

References 65 publications

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“…Total lipid was extracted according to Lefebvre et al (2022). Extractions were carried out using freeze‐dried SE (about 20 mg FW) ground with 0.5 mL chloroform:methanol (1:2).…”

Section: Methodsmentioning

confidence: 99%

“…Then, we aimed to develop models to discriminate SE depending on the time they spent in the maturation medium in order to facilitate the comparison of SE based on their developmental status. For this purpose, SE were characterized for their biochemical constituents during maturation using traditional laboratory methods (Lefebvre et al, 2022; Morel, Teyssier, et al, 2014; Teyssier et al, 2011). At the same time, different samples were taken to develop calibration models in fresh SE based on mid‐IR spectra obtained from a single hybrid larch SE.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of hybrid larch somatic embryo maturation by biochemical analyses and by a novel, fast mid‐infrared approach

Savane

Belmokhtar

Delile

et al. 2023

Physiologia Plantarum

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The morphology of somatic embryos (SE) is not a sufficient criterion to determine the level of maturation and the optimal stage to transfer embryos for germination, unlike the biochemical components. This composition characterization in the laboratory is too restrictive to be considered at each maturation cycle, as would be necessary. It is, therefore, essential to consider alternative methods. The objectives of this work were to achieve a complete biochemical characterization of the embryos during their development, to serve as a reference and develop a characterization based on infrared spectrometry and chemometrics. During the precotyledonary stage (0–3 weeks of maturation), water content and glucose and fructose levels were high, which is consistent with SE development. After 4 weeks, the cotyledonary SE had a metabolism oriented towards the storage accumulation of lipids, proteins and starch, whereas raffinose only appeared from 8 weeks. Mid‐infrared calibration models were developed for water, proteins, lipids, carbohydrates, glucose, fructose, inositols, raffinose, stachyose and starch contents with an r2 average of 0.84. A model was also developed to discriminate the weeks of SE maturation. Different classes of age were discriminated with at least 72% of accuracy. Infrared analysis of the SE based on their full biochemical spectral fingerprint revealed a very slight variation in composition between 7 and 9 weeks, information that is very difficult to obtain by conventional analysis methods. These results provide novel insights into the maturation of conifer SE and indicate that mid‐infrared spectrometry could be an easy and effective method for SE characterization.

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“…Total lipid was extracted according to Lefebvre et al (2022). Extractions were carried out using freeze‐dried SE (about 20 mg FW) ground with 0.5 mL chloroform:methanol (1:2).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of hybrid larch somatic embryo maturation by biochemical analyses and by a novel, fast mid‐infrared approach

Savane

Belmokhtar

Delile

et al. 2023

Physiologia Plantarum

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“…In this context, black poplar plays a major role in the development of riparian forests in European strategies [ 2 ]. The sexual regeneration of riparian forests using seedlings is essential for gene flow and in order to enhance disease resistance and adaptation to environmental changes through the natural genetic variation of populations [ 3 ]. However, the survival of poplar seedlings depends on the combination of many factors.…”

Section: Introductionmentioning

confidence: 99%

Black Poplar (Populus nigra L.) Root Extracellular Trap, Structural and Molecular Remodeling in Response to Osmotic Stress

Busont

Durambur

Bernard

et al. 2023

Cells

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The root extracellular trap (RET) consists of root-associated, cap-derived cells (root AC-DCs) and their mucilaginous secretions, and forms a structure around the root tip that protects against biotic and abiotic stresses. However, there is little information concerning the changes undergone by the RET during droughts, especially for tree species. Morphological and immunocytochemical approaches were used to study the RET of black poplar (Populus nigra L.) seedlings grown in vitro under optimal conditions (on agar-gelled medium) or when polyethylene glycol-mediated (PEG6000—infused agar-gelled medium) was used to mimic drought conditions through osmotic stress. Under optimal conditions, the root cap released three populations of individual AC-DC morphotypes, with a very low proportion of spherical morphotypes, and equivalent proportions of intermediate and elongated morphotypes. Immunolabeling experiments using anti-glycan antibodies specific to cell wall polysaccharide and arabinogalactan protein (AGP) epitopes revealed the presence of homogalacturonan (HG), galactan chains of rhamnogalacturonan-I (RG-I), and AGPs in root AC-DC cell walls. The data also showed the presence of xylogalacturonan (XGA), xylan, AGPs, and low levels of arabinans in the mucilage. The findings also showed that under osmotic stress conditions, both the number of AC-DCs (spherical and intermediate morphotypes) and the total quantity of mucilage per root tip increased, whereas the mucilage was devoid of the epitopes associated with the polysaccharides RG-I, XGA, xylan, and AGPs. Osmotic stress also led to reduced root growth and increased root expression of the P5CS2 gene, which is involved in proline biosynthesis and cellular osmolarity maintenance (or preservation) in aerial parts. Together, our findings show that the RET is a dynamic structure that undergoes pronounced structural and molecular remodeling, which might contribute to the survival of the root tip under osmotic conditions.

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“…Despite potential differences in aging mechanisms between P. nigra and A. thaliana, black poplar seeds have been chosen for this research as this species is one of the most endangered tree species in Europe [17]. In Poland, this species is not legally protected as a species, but most populations are part of the Natura 2000 conservation program that prevents devastation of ecologically valuable ecosystems.…”

Section: Introductionmentioning

confidence: 99%

“…Black poplar seeds rapidly lose their viability in storage [22,23], although it is possible to extend the span of their seed viability up to 1-2 years [24,25]. In relation to aging, the physiological and biochemical changes in P. nigra seeds have been the subject of several studies [17,[23][24][25][26][27][28]. Specifically, these studies have demonstrated that storage of black poplar seeds results in increased oxidative stress [27], large changes in protein abundance [28], and a reduced proportion of roots with absorptive function [26].…”

Section: Introductionmentioning

confidence: 99%

DNA Methylation as an Early Indicator of Aging in Stored Seeds of “Exceptional” Species Populus nigra L.

Michalak

Plitta-Michalak

Naskręt-Barciszewska

et al. 2022

Cells

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Ex situ preservation of genetic resources is an essential strategy for the conservation of plant biodiversity. In this regard, seed storage is the most convenient and efficient way of preserving germplasm for future plant breeding efforts. A better understanding of the molecular changes that occur during seed desiccation and aging is necessary to improve conservation protocols, as well as real-time methods for monitoring seed quality. In the present study, we assessed changes in the level of genomic 5-methylcytosine (5mC) in seeds of Populus nigra L. by 2D-TLC. Epigenetic changes were characterized in response to several seed storage regimes. Our results demonstrate that P. nigra seeds represent an intermediate type of post-harvest behavior, falling between recalcitrant and orthodox seeds. This was also true for the epigenetic response of P. nigra seeds to external factors. A crucial question is whether aging in seeds is initiated by a decline in the level of 5mC, or if epigenetic changes induce a process that leads to deterioration. In our study, we demonstrate for the first time that 5mC levels decrease during storage and that the decline can be detected before any changes in seed germination are evident. Once P. nigra seeds reached an 8–10% reduction in the level of 5mC, a substantial decrease in germination occurred. The decline in the level of 5mC appears to be a critical parameter underlying the rapid deterioration of intermediate seeds. Thus, the measurement of 5mC can be a fast, real-time method for assessing asymptomatic aging in stored seeds.

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Variability in seeds’ physicochemical characteristics, germination and seedling growth within and between two French Populus nigra L. populations

Abstract: Variability in seeds' physicochemical characteristics, germination and seedling growth within and between twoFrench Populus nigra L. populations, Peer Community Journal, 2: e10.

Cited by 4 publications

References 65 publications

Characterization of hybrid larch somatic embryo maturation by biochemical analyses and by a novel, fast mid‐infrared approach

Characterization of hybrid larch somatic embryo maturation by biochemical analyses and by a novel, fast mid‐infrared approach

Black Poplar (Populus nigra L.) Root Extracellular Trap, Structural and Molecular Remodeling in Response to Osmotic Stress

DNA Methylation as an Early Indicator of Aging in Stored Seeds of “Exceptional” Species Populus nigra L.

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