Variability of an Early Developmental Cell Population Underlies Stochastic Laterality Defects

Moreno-Ayala, Roberto; Olivares-Chauvet, Pedro; Schäfer, Ronny; Junker, Jan Philipp

doi:10.1016/j.celrep.2020.108606

Cited by 15 publications

(12 citation statements)

References 50 publications

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“…recent study looking at a sub-population of functionally distinct endoderm cells in early zebrafish embryos found similar fluctuations in the total cell number between individual embryos (Moreno-Ayala et al, 2021). This suggests that individual fluctuations in the absolute number of specialized cells within a tissue is a common phenomenon in early (zebrafish) development.…”

Section: Discussionmentioning

confidence: 72%

A quantitative characterization of early neuron generation in the developing zebrafish telencephalon

Gimeno

Dvorianinova

Lembke

et al. 2023

Preprint

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The adult brain is made up of anatomically and functionally distinct regions with specific neuronal compositions. At the root of this neuronal diversity are neural stem and progenitor (NPCs) cells that produce many neurons throughout embryonic development. During development, NPCs switch from initial expanding divisions to neurogenic divisions, which marks the onset of neurogenesis. Here, we aimed to understand when NPCs switch division modes to generate the first neurons in the anterior-most part of the zebrafish brain, the telencephalon. To this end, we used the deep learning-based segmentation method Cellpose and clonal analysis of individual NPCs to assess production of neurons by NPCs in the first 24 hours of zebrafish telencephalon development. Our results provide a quantitative atlas detailing the production of telencephalic neurons and NPC division modes between 14 and 24 hours post-fertilization. We find that within this timeframe, the switch to neurogenesis is gradual, with considerable heterogeneity in individual NPC neurogenic potential and division rates. This quantitative characterization of initial neurogenesis in the zebrafish telencephalon establishes a basis for future studies aimed at illuminating the molecular mechanisms and regulators of early neurogenesis.

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Section: Discussionmentioning

confidence: 72%

A quantitative characterization of early neuron generation in the developing zebrafish telencephalon

Gimeno

Dvorianinova

Lembke

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…However, the variation in total neuron numbers between individual embryos of the same stage may have a biological component. Strikingly, a recent study looking at a subpopulation of functionally distinct endoderm cells in early zebrafish embryos found similar fluctuations in the total cell number between individual embryos (Moreno‐Ayala et al., 2021). This suggests that individual fluctuations in the absolute number of specialized cells within a tissue are a common phenomenon in early (zebrafish) development.…”

Section: Discussionmentioning

confidence: 83%

A quantitative characterization of early neuron generation in the developing zebrafish telencephalon

Casas Gimeno,

Dvorianinova,

Lembke

et al. 2023

Developmental Neurobiology

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The adult brain is made up of anatomically and functionally distinct regions with specific neuronal compositions. At the root of this neuronal diversity are neural stem and progenitor cells (NPCs) that produce many neurons throughout embryonic development. During development, NPCs switch from initial expanding divisions to neurogenic divisions, which marks the onset of neurogenesis. Here, we aimed to understand when NPCs switch division modes to generate the first neurons in the anterior‐most part of the zebrafish brain, the telencephalon. To this end, we used the deep learning‐based segmentation method Cellpose and clonal analysis of individual NPCs to assess the production of neurons by NPCs in the first 24 h of zebrafish telencephalon development. Our results provide a quantitative atlas detailing the production of telencephalic neurons and NPC division modes between 14 and 24 h postfertilization. We find that within this timeframe, the switch to neurogenesis is gradual, with considerable heterogeneity in individual NPC neurogenic potential and division rates. This quantitative characterization of initial neurogenesis in the zebrafish telencephalon establishes a basis for future studies aimed at illuminating the molecular mechanisms and regulators of early neurogenesis.

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“…3A-I). As in zebrafish (Moreno-Ayala et al, 2021), DFC numbers varied greatly within Astyanax SF-D, CF-D, and CF-L/D embryos (Fig 3I). However, the mean number of DFC was significantly larger in CF-L/D embryos relative to CF-D and SF-D embryos (Fig.…”

Section: Dorsal Forerunner Cells and Foxj1a Expression Are Increased ...mentioning

confidence: 85%

Natural reversal of cavefish heart asymmetry is controlled by Sonic Hedgehog effects on the left-right organizer

Ng,

Ma,

Shi

et al. 2023

Preprint

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The direction of left-right (L-R) visceral asymmetry is conserved in vertebrates. Deviations of the asymmetric pattern are rare, and the underlying mechanisms are not understood. Here we use the teleostAstyanax mexicanus, a single species consisting of surface fish with normal left-oriented heart asymmetry and cavefish with high levels of reversed right-oriented heart asymmetry, to explore natural changes in L-R determination. We show that Sonic Hedgehog (Shh) signaling is increased at the posterior midline, Kupffers Vesicle (KV), the teleost L-R Organizer (LRO), is enlarged and contains longer cilia, and the number of dorsal forerunner cells, the KV precursors, is increased in cavefish. Furthermore, Shh overexpression in surface fish induces KV modifications and changes in heart asymmetry resembling the cavefish phenotype. Asymmetric expression of the Nodal antagonistdand5is equalized or reversed in the cavefish KV, and Shh overexpression in surface fish mimics changes in cavefish L-Rdand5asymmetry and shifts Nodal laterality in the lateral plate mesoderm. Our results show that naturally occurring modifications in cavefish heart asymmetry are controlled by the effects of enhanced Shh signaling on LRO function.SummaryNatural reversal of cavefish heart asymmetry is controlled by enhanced Sonic Hedgehog signaling at the posterior midline, which modifies left-right organizer function and subsequent Nodal laterality.

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Variability of an Early Developmental Cell Population Underlies Stochastic Laterality Defects

Cited by 15 publications

References 50 publications

A quantitative characterization of early neuron generation in the developing zebrafish telencephalon

A quantitative characterization of early neuron generation in the developing zebrafish telencephalon

A quantitative characterization of early neuron generation in the developing zebrafish telencephalon

Natural reversal of cavefish heart asymmetry is controlled by Sonic Hedgehog effects on the left-right organizer

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